全文获取类型
收费全文 | 1478篇 |
免费 | 129篇 |
专业分类
1607篇 |
出版年
2023年 | 5篇 |
2022年 | 25篇 |
2021年 | 41篇 |
2020年 | 17篇 |
2019年 | 25篇 |
2018年 | 33篇 |
2017年 | 28篇 |
2016年 | 47篇 |
2015年 | 58篇 |
2014年 | 62篇 |
2013年 | 90篇 |
2012年 | 143篇 |
2011年 | 115篇 |
2010年 | 86篇 |
2009年 | 76篇 |
2008年 | 90篇 |
2007年 | 111篇 |
2006年 | 83篇 |
2005年 | 94篇 |
2004年 | 94篇 |
2003年 | 63篇 |
2002年 | 68篇 |
2001年 | 8篇 |
2000年 | 8篇 |
1999年 | 11篇 |
1998年 | 16篇 |
1997年 | 14篇 |
1996年 | 9篇 |
1995年 | 12篇 |
1994年 | 6篇 |
1993年 | 4篇 |
1992年 | 5篇 |
1991年 | 5篇 |
1990年 | 3篇 |
1987年 | 5篇 |
1986年 | 2篇 |
1984年 | 4篇 |
1983年 | 5篇 |
1982年 | 6篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1976年 | 2篇 |
1974年 | 2篇 |
1961年 | 2篇 |
1960年 | 1篇 |
1959年 | 1篇 |
1958年 | 3篇 |
1957年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有1607条查询结果,搜索用时 15 毫秒
291.
292.
Milstein Oleg Vered Yaffa Shragina Lea Gressel Jonathan Flowers Harold M. Hüttermann Aloys 《Archives of microbiology》1983,136(2):147-151
Archives of Microbiology - Aspergillus japonicus metabolizes a wide variety of aromatic compounds, some of them resulting from lignin degradation. The efficient conversion of such compounds is... 相似文献
293.
294.
We describe electron microscopy (EM), scanning transmission electron microscopy (STEM), and solid-state nuclear magnetic resonance (NMR) measurements on amyloid fibrils formed by the 42-residue beta-amyloid peptide associated with Alzheimer's disease (Abeta(1)(-)(42)) and by residues 10-35 of the full-length peptide (Abeta(10)(-)(35)). These measurements place constraints on the supramolecular structure of the amyloid fibrils, especially the type of beta-sheets present in the characteristic amyloid cross-beta structural motif and the assembly of these beta-sheets into a fibril. EM images of negatively stained Abeta(10)(-)(35) fibrils and measurements of fibril mass per length (MPL) by STEM show a strong dependence of fibril morphology and MPL on pH. Abeta(10)(-)(35) fibrils formed at pH 3.7 are single "protofilaments" with MPL equal to twice the value expected for a single cross-beta layer. Abeta(10)(-)(35) fibrils formed at pH 7.4 are apparently pairs of protofilaments or higher order bundles. EM and STEM data for Abeta(1)(-)(42) fibrils indicate that protofilaments with MPL equal to twice the value expected for a single cross-beta layer are also formed by Abeta(1)(-)(42) and that these protofilaments exist singly and in pairs at pH 7.4. Solid-state NMR measurements of intermolecular distances in Abeta(10)(-)(35) fibrils, using multiple-quantum (13)C NMR, (13)C-(13)C dipolar recoupling, and (15)N-(13)C dipolar recoupling techniques, support the in-register parallel beta-sheet organization previously established by Lynn, Meredith, Botto, and co-workers [Benzinger et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95, 13407-13412; Benzinger et al. (2000) Biochemistry 39, 3491-3499] and show that this beta-sheet organization is present at pH 3.7 as well as pH 7.4 despite the differences in fibril morphology and MPL. Solid-state NMR measurements of intermolecular distances in Abeta(1)(-)(42) fibrils, which represent the first NMR data on Abeta(1)(-)(42) fibrils, also indicate an in-register parallel beta-sheet organization. These results, along with previously reported data on Abeta(1)(-)(40) fibrils, suggest that the supramolecular structures of Abeta(10)(-)(35), Abeta(1)(-)(40), and Abeta(1)(-)(42) fibrils are quite similar. A schematic structural model of these fibrils, consistent with known experimental EM, STEM, and solid-state NMR data, is presented. 相似文献
295.
296.
The fatty acids of human depot fat 总被引:1,自引:0,他引:1
297.
Prasert Laobusnanant Seung Hwan Lee Alfredo J. Anceno Gopal C. Ghosh Dong Jin Kim Bipin K. Pathak Oleg V. Shipin 《Bioprocess and biosystems engineering》2009,32(6):809-818
Biological N-removal treatment of piggery wastewater in the upflow anaerobic–anoxic–aerobic floating filter (UA3FF) bioreactor based on the concept of nitritation–denitritation was studied along with the changes in internal recycle ratio
and dissolved oxygen concentration (DO). Consecutive changes in the recirculation ratio between the anoxic and aerobic reactors
has resulted in abundance and composition shifts of N-cycling bacteria as well as other bacterial groups, reflecting different
survival strategies across (bio/physico)chemical milieu. The DO concentration was optimized to achieve nitritation in the
aerobic reactor and denitritation in the anoxic reactor. Optimal nitritation–denitritation (270 and 130 g NO2
−–N produced or reduced/m3 filter media/day) was obtained at DO of 1.0–1.5 mg/l, inter-reactor recirculation ratio of 1:1–2:1, HRT of 24 h, pH of 7.6 ± 0.3,
and temperature of 28 ± 4 °C. Since only well known nitrifying and denitrifying taxa were found, nitritation–denitritation
was likely carried out by these bacteria rather than the yet unidentified novel taxa. Archaeal nitrifiers recently discovered
to be important in the global N-cycle were not detected. 相似文献
298.
Hacne Medkour Inestin Amona Jean Akiana Younes Laidoudi Bernard Davoust Idir Bitam Ismail Lafri Anthony Levasseur Georges Diatta Cheikh Sokhna R. Adriana Hernandez-Aguilar Amanda Barciela Slim Gorsane Henri Banga-Mboko Didier Raoult Florence Fenollar Oleg Mediannikov 《The Yale journal of biology and medicine》2021,94(2):227
The close phylogenetic relationship between humans and other primates creates exceptionally high potential for pathogen exchange. The surveillance of pathogens in primates plays an important role in anticipating possible outbreaks. In this study, we conducted a molecular investigation of pathogenic bacteria in feces from African nonhuman primates (NHPs). We also investigated the pathogens shared by the human population and gorillas living in the same territory in the Republic of Congo. In total, 93% of NHPs (n=176) and 95% (n=38) of humans were found to carry at least one bacterium. Non-pallidum Treponema spp. (including T. succinifaciens, T. berlinense, and several potential new species) were recovered from stools of 70% of great apes, 88% of monkeys, and 79% of humans. Non-tuberculosis Mycobacterium spp. were also common in almost all NHP species as well as in humans. In addition, Acinetobacter spp., members of the primate gut microbiota, were mainly prevalent in human and gorilla. Pathogenic Leptospira spp. were highly present in humans (82%) and gorillas (66%) stool samples in Congo, but were absent in the other NHPs, therefore suggesting a possible gorillas-humans exchange. Particular attention will be necessary for enteropathogenic bacteria detected in humans such as Helicobacter pylori, Salmonella spp. (including S. typhi/paratyphi), Staphyloccocus aureus, and Tropheryma whipplei, some of which were also present in gorillas in the same territory (S. aureus and T. whipplei). This study enhances our knowledge of pathogenic bacteria that threaten African NHPs and humans by using a non-invasive sampling technique. Contact between humans and NHPs results in an exchange of pathogens. Ongoing surveillance, prevention, and treatment strategies alone will limit the spread of these infectious agents. 相似文献
299.
300.
Agnieszka A. Kusiak Monika A. Jakubowska Kinga B. Stopa Xiaoying Zhang Wei Huang Julia V. Gerasimenko Oleg V. Gerasimenko Robert Sutton Ole H. Petersen Pawel E. Ferdek 《Cell death & disease》2022,13(8)
Alcohol abuse, an increasing problem in developed societies, is one of the leading causes of acute and chronic pancreatitis. Alcoholic pancreatitis is often associated with fibrosis mediated by activated pancreatic stellate cells (PSCs). Alcohol toxicity predominantly depends on its non-oxidative metabolites, fatty acid ethyl esters, generated from ethanol and fatty acids. Although the role of non-oxidative alcohol metabolites and dysregulated Ca2+ signalling in enzyme-storing pancreatic acinar cells is well established as the core mechanism of pancreatitis, signals in PSCs that trigger fibrogenesis are less clear. Here, we investigate real-time Ca2+ signalling, changes in mitochondrial potential and cell death induced by ethanol metabolites in quiescent vs TGF-β-activated PSCs, compare the expression of Ca2+ channels and pumps between the two phenotypes and the consequences these differences have on the pathogenesis of alcoholic pancreatitis. The extent of PSC activation in the pancreatitis of different aetiologies has been investigated in three animal models. Unlike biliary pancreatitis, alcohol-induced pancreatitis results in the activation of PSCs throughout the entire tissue. Ethanol and palmitoleic acid (POA) or palmitoleic acid ethyl ester (POAEE) act directly on quiescent PSCs, inducing cytosolic Ca2+ overload, disrupting mitochondrial functions, and inducing cell death. However, activated PSCs acquire remarkable resistance against ethanol metabolites via enhanced Ca2+-handling capacity, predominantly due to the downregulation of the TRPA1 channel. Inhibition or knockdown of TRPA1 reduces EtOH/POA-induced cytosolic Ca2+ overload and protects quiescent PSCs from cell death, similarly to the activated phenotype. Our results lead us to review current dogmas on alcoholic pancreatitis. While acinar cells and quiescent PSCs are prone to cell death caused by ethanol metabolites, activated PSCs can withstand noxious signals and, despite ongoing inflammation, deposit extracellular matrix components. Modulation of Ca2+ signals in PSCs by TRPA1 agonists/antagonists could become a strategy to shift the balance of tissue PSCs towards quiescent cells, thus limiting pancreatic fibrosis.Subject terms: Cell death, Ion channel signalling, Gastrointestinal diseases, Preclinical research, Physiology 相似文献