排序方式: 共有17条查询结果,搜索用时 15 毫秒
11.
S. V. Kolobanova I. Yu. Filippova E. N. Lysogorskaya I. V. Getun A. V. Bacheva E. S. Oksenoit V. M. Stepanov 《Russian Journal of Bioorganic Chemistry》2000,26(6):369-374
The subtilisin-sodium dodecyl sulfate complex was shown to catalyze the coupling of peptide segments on a solid phase in organic
medium. By a two-stage enzymic condensation of peptide fragments on aminosilochrom (A) containing Met-Ala-Gly as a spacer, Dnp(or Boc)-Ala-Ala-Leu-Ala-Ala-Glu(OMe)-Met-Ala-Gly-A and Z-Ala-Ala-Glu(OMe)-Ala-Ala-Leu-Met-Ala-Gly-A were obtained. It was shown that the condensation products can be split off from the support using Met residue cleavage by
BrCN.
Deceased. 相似文献
12.
Irina V. Getun Irina Yu. Filippova Elena N. Lysogorskaya Elena S. Oksenoit 《Journal of Molecular Catalysis .B, Enzymatic》2001,15(4-6):105-110
The role of acyl donor structure on the course of peptide bond formation catalyzed by SDS-subtilisin in ethanol was investigated. In the reaction Z---Ala---Ala---Leu---OR+H---Phe---pNA→Z---Ala---Ala---Leu---Phe---pNA, nearly quantitative product yields were observed after 2 h, regardless of whether an activated (R=CH3, p-C6H5Cl) or non-activated (R=H) acyl donor was used. It was found that the enzyme can accept as acyl donors N-protected tri-peptides containing basic or acidic amino acid residues in the P1-position. Tetra-peptides of general formula Z---Ala---Ala---P1---P1′---pNA, where P1=Glu, Asp, Lys, Arg or His and P1′=Phe, Arg or Glu have been obtained in good yield. 相似文献
13.
I. Yu. Filippova E. N. Lysogorskaya G. I. Lavrenova E. S. Oksenoit L. I. Suvorov V. V. Starovoitova 《Russian Journal of Bioorganic Chemistry》2000,26(3):169-173
A series of fluorogenic tetra-, penta-, and hexapeptide substrates of the general structure Abz-X-Phe-Phe-Y-Ded or (-pNa in
place of -Ded), where X=Ala, Ala-Ala, or Val-Ala and Y=−, Ala, or Ala-Ala, were proposed. Kinetic parameters of hydrolysis
of these substrates by pepsin, cathepsin D, human gastricsin, pig pepsin, calf chymosin, and aspergillopepsin A were determined.
The compounds synthesized proved to be effective substrates for aspartyl proteases of diverse origins. 相似文献
14.
Properties of post-proline cleaving enzymes from <Emphasis Type="Italic">Tenebrio molitor</Emphasis>
I. A. Goptar I. A. Koulemzina I. Yu. Filippova E. N. Lysogorskaya E. S. Oksenoit D. P. Zhuzhikov Ya. E. Dunaevsky M. A. Belozersky E. N. Elpidina 《Russian Journal of Bioorganic Chemistry》2008,34(3):280-285
Two post-proline cleaving peptidases PPCP1 and PPCP2 with molecular masses of 101 and 63 kDa, respectively, hydrolyzing Z-AlaAlaPro-pNA were isolated for the first time from the larval midgut of the yellow mealworm Tenebrio molitor and characterized. PPCP1 was active only in acidic media, with a maximum at pH 5.6, whereas PPCP2, both in acidic and alkaline media with a maximum at pH 7.9. Using inhibitory analysis, both PPCP1 and PPCP2 were shown to belong to serine peptidases. The data obtained indicate that a Cys residue is located close to the PPCP2 substrate binding site. Z-Pro-prolinal, a specific inhibitor of prolyl oligopeptidases, completely inhibited PPCP2 and partially PPCP1. The substrate specificities of the isolated enzymes were studied. Z-Ala-Ala-Pro-pNA was the best substrate for PPCP1, and Z-Ala-Pro-pNA, for PPCP2. The combination of the properties allows characterization of PPCP2 as a proplyl oligopeptidase. 相似文献
15.
A.?V.?Belyaeva A.?V.?Bacheva E.?S.?Oksenoit E.?N.?Lysogorskaya V.?I.?Lozinskii I.?Yu.?FilippovaEmail author 《Russian Journal of Bioorganic Chemistry》2005,31(6):529-534
Subtilisin 72 serine protease (EC 3.4.21.14) immobilized on a poly(vinyl alcohol) cryogel was used as a catalyst in the syntheses of N-protected peptide p-nitroanilides of the general formulas Z(or Boc)-Xaa-Phe-pNA (Xaa = Leu or Ala), Z-Ala-Xaa-Yaa-pNA (Xaa = Leu or Ala; Yaa = Leu or Phe), and Z-Ala-Ala-Xaa-Yaa-pNA (Xaa = Leu, Arg, or Gly; Yaa = Phe, Leu, Gly, Asp, or Glu). The syntheses were carried out in DMF-acetonitrile mixtures. A number of protected di-, tri-, and tetrapeptides were prepared in yields up to 99%. The syntheses were found to retain stereoselectivity under the conditions studied. The activation of carboxyl group of the acylating component was shown to have a positive effect upon the coupling rate. 相似文献
16.
Bacheva AV Baibak OV Belyaeva AV Oksenoit ES Velichko TI Lysogorskaya EN Gladilin AK Lozinsky VI Filippova IY 《Biochemistry. Biokhimii?a》2003,68(11):1261-1266
The activity and stability of native subtilisin 72, its complex with poly(acrylic acid), and subtilisin covalently attached to poly(vinyl alcohol) cryogel were studied in aqueous and organic media by hydrolysis of specific chromogenic peptide substrates. Kinetic parameters of the hydrolysis of Glp-Ala-Ala-Leu-pNA by native subtilisin and its complex with poly(acrylic acid) were determined. Based on the comparative study of stability of native and modified subtilisins in media of various compositions, it was established that covalent immobilization of subtilisin on poly(vinyl alcohol) cryogel is the most effective approach to improve enzyme stability in water as well as in mixtures with low water content. 相似文献
17.
Starovoitova VV Filippova IY Lysogorskaya EN Oksenoit ES Lavrenova GI 《Biochemistry. Biokhimii?a》2000,65(6):713-717
The use of fluorogenic substrates with intramolecular fluorescence quenching as substrates for chymosin was studied. It was shown that chymosin hydrolyzes the Phe-Phe peptide bond. The effect of pH on the hydrolysis of substrates by chymosin was investigated. The catalytic characteristics of the hydrolysis of the fluorogenic substrates were obtained at the pH optima. The influence of dimethylformamide on chymosin activity was studied. 相似文献