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51.
Background
We have previously investigated the microlocalisation of M1 and M2 macrophages in NSCLC. This study investigated the non-macrophage (NM) expression of proteins associated with M1 and M2 macrophages in NSCLC.Methods
Using immunohistochemistry, CD68+ macrophages and proteins associated with either a cytotoxic M1 phenotype (HLA-DR, iNOS, and MRP 8/14), or a non-cytotoxic M2 phenotype (CD163 and VEGF) were identified. NM expression of the markers was analysed in the islets and stroma of surgically resected tumours from 20 patients with extended survival (ES) (median 92.7 months) and 20 patients with poor survival (PS) (median 7.7 months).Results
The NM expression of NM-HLA-DR (p<0.001), NM-iNOS (p = 0.02) and NM-MRP 8/14 (p = 0.02) was increased in ES compared to PS patients in the tumour islets. The tumour islet expression of NM-VEGF, was decreased in ES compared to PS patients (p<0.001). There was more NM-CD163 expression (p = 0.04) but less NM-iNOS (p = 0.002) and MRP 8/14 (p = 0.01) expression in the stroma of ES patients compared with PS patients. The 5-year survival for patients with above and below median NM expression of the markers in the islets was 74.9% versus 4.7% (NM-HLA-DR p<0.001), 65.0% versus 14.6% (NM-iNOS p = 0.003), and 54.3% versus 22.2% (NM-MRP 8/14 p = 0.04), as opposed to 34.1% versus 44.4% (NM-CD163 p = 0.41) and 19.4% versus 59.0% (NM-VEGF p = 0.001).Conclusions
Cell proteins associated with M1 and M2 macrophages are also expressed by other cell types in the tumour islets and stroma of patients with NSCLC. Their tissue and cellular microlocalisation is associated with important differences in clinical outcome. 相似文献52.
Smith-Lemli-Opitz syndrome is an autosomal recessively inherited disorder. A severe defect in cholesterol biosynthesis has been identified leading to abnormally low plasma cholesterol levels and elevated levels of the cholesterol precursor 7-dehydrocholesterol, the result of deficiency of 7-dehydrocholesterol reductase. We describe one such child with Smith-Lemli-Opitz syndrome. This child had clinical features similar to Smith-Lemli-Opitz syndrome like facial dysmorphism and cardiac and renal anomalies with failure to thrive. 相似文献
53.
Sei Kwang Hahn Rachit Ohri Cecilia M. Giachelli 《Biotechnology and Bioprocess Engineering》2005,10(3):218-224
Surface modification of glutaraldehyde fixed bovine pericardium (GFBP) was successfully carried out with hyaluronic acid (HA)
derivatives. At first, HA was chemically modified with adipic dihydrazide (ADH) to introduce hydrazide functional group into
the carboxyl group of HA backbone. Then, GFBP was surface modified by grafting HA-ADH to the free aldehyde groups on the tissue
and the subsequent HA-ADH hydrogel coating. HA-ADH hydrogels could be prepared through selective crosslinking at low pH between
hydrazide groups of HA-ADH and crosslinkers containing succinimmidyl moieties with minimized protein denaturation. When HA-ADH
hydrogels were prepared at low pH of 4.8 in the presence of erythropoietin (EPO) as a model protein, EPO release was continued
up to 85% of total amount of loaded EPO for 4 days. To the contrary, only 30% of EPO was released from HA-ADH hydrogels prepared
at pH=7.4, which might be due to the denaturation of EPO during the crosslinking reaction. Because the carboxyl groups on
the glucuronic acid residues are recognition sites for HA degradation by hyaluronidase, the HA-ADH hydrogels degraded more
slowly than HA hydrogels prepared by the crosslinking reaction of divinyl sulfone with hydroxyl groups of HA. Following a
two-week subcutaneous implantation in osteopontin-null mice, clinically significant levels of calcification were observed
for the positive controls without any surface modification. However, the calcification of surface modified GFBP with HA-ADH
and HA-ADH hydrogels was drastically reduced by more than 85% of the positive controls. The anti-calcification effect of HA
surface modification was also confirmed by microscopic analysis of explan ted tissue after staining with Alizarin Red S for
calcium, which followed the trend as observed with calcium quantification. 相似文献
54.
Moghadam M. Ayati H. Shobeiri SS. Rajabian M. Rahbarian R. Sankian M. 《Applied Biochemistry and Microbiology》2022,58(4):443-448
Applied Biochemistry and Microbiology - Vit v 1 as a lipid-transfer protein is a major allergen of grapes (Vitis vinifera) that elicits food allergy in many patients in Iran. Todays, recombinant... 相似文献
55.
The present study was undertaken to understand the genetic differentiation and relationships in various components of C. album complex, C. giganteum and some related species using inter simple sequence repeats (ISSR) profiles and internal transcribed spacer (ITS) sequences. The relationships based on UPGMA dendrograms have shown the heterogenous nature of C. album complex. The 2x taxa while showing close relation among themselves are sharply segregated from 4x and 6x taxa belonging to C. album and C. giganteum. Among the three cytotypes from North Indian plains the 4x shows greater similarity to 6x than to 2x which is corroborated by the karyotypic studies. Furthermore, the 6x C. album and C. giganteum accessions of American and European origin are clearly segregated from those of Indian origin which may show their separate origin. Other related species show relationships according to their taxonomic position. The present study based on ISSR profiles and ITS sequences has therefore been very useful in explaining the relationships between various components of C. album complex and related species. However, more work needs to be done using different CpDNA loci to define correct species boundary of the taxa under C. album complex from Himalayas and North Indian Plains. 相似文献
56.
Chen Y Chalouni C Tan C Clark R Venook R Ohri R Raab H Firestein R Mallet W Polakis P 《The Journal of biological chemistry》2012,287(29):24082-24091
Melanocytes uniquely express specialized genes required for pigment formation, some of which are maintained following their transformation to melanoma. Here we exploit this property to selectively target melanoma with an antibody drug conjugate (ADC) specific to PMEL17, the product of the SILV pigment-forming gene. We describe new PMEL17 antibodies that detect the endogenous protein. These antibodies help define the secretory fate of PMEL17 and demonstrate its utility as an ADC target. Although newly synthesized PMEL17 is ultimately routed to the melanosome, we find substantial amounts accessible to our antibodies at the cell surface that undergo internalization and routing to a LAMP1-enriched, lysosome-related organelle. Accordingly, an ADC reactive with PMEL17 exhibits target-dependent tumor cell killing in vitro and in vivo. 相似文献
57.
The 4C DNA values of 112 species, belonging to 37 families have a range from 0.83 pg (Bixa orellana) to 15.54 pg (Thryallis angustifolia), showing a 18.72-fold variation. The genome size varies from 0.21 pg (Bixa orellana) to 3.32 (Thespesia populnea), with a 15.8-fold difference. The Bombacaceae has the minimum range (1.08-fold) of variation, while the maximum (5.0-fold) is shown by the Fabaceae. The Boraginaceae, Lauraceae, Malpighiaceae, and Malvaceae generally have higher 4C DNA values of > 10 pg, while the Bixaceae, Caricaceae, Oxalidaceae, and Santalaceae have lower values of < 2.0 pg. These data add further to our knowledge on variation in DNA amount in tropical hardwoods. 相似文献
58.
Jasmonic acid-induced tolerance to root-knot nematodes in tomato plants through altered photosynthetic and antioxidative defense mechanisms 总被引:2,自引:0,他引:2
Shagun?Bali Parminder?Kaur Anket?Sharma Puja?Ohri Renu?BhardwajEmail author M.?N.?Alyemeni Leonard?Wijaya Parvaiz?AhmadEmail author 《Protoplasma》2018,255(2):471-484
Plant parasitic nematodes cause severe damage to cultivated crops globally. Management of nematode population is a major concern as chemicals used as nematicides have negative impact on the environment. Natural plant products can be safely used for the control of nematodes. Among various plant metabolites, plant hormones play an essential role in developmental and physiological processes and also assist the plants to encounter stressful conditions. Keeping this in mind, the present study was designed to evaluate the effect of jasmonic acid (JA) on the growth, pigments, polyphenols, antioxidants, osmolytes, and organic acids under nematode infection in tomato seedlings. It was observed that nematode inoculation reduced the growth of seedlings. Treatment with JA improved root growth (32.79%), total chlorophylls (71.51%), xanthophylls (94.63%), anthocyanins (37.5%), and flavonoids content (21.11%) when compared to inoculated seedlings alone. The JA application enhanced the total antioxidant capacity (lipid- and water-soluble antioxidants) by 38.23 and 34.37%, respectively, in comparison to infected seedlings. Confocal studies revealed that there was higher accumulation of glutathione in hormone-treated seedlings under nematode infection. Treatment with JA increased total polyphenols content (74.56%) in comparison to nematode-infested seedlings. JA-treated seedlings also enhanced osmolyte and organic acid contents under nematode stress. Overall, treatment with JA improved growth, enhanced pigment levels, modulated antioxidant content, and enhanced osmolyte and organic acid content in nematode-infected seedlings. 相似文献
59.
Procathepsin D secreted by HaCaT keratinocyte cells - A novel regulator of keratinocyte growth 总被引:1,自引:1,他引:0
Vashishta A Saraswat Ohri S Vetvickova J Fusek M Ulrichova J Vetvicka V 《European journal of cell biology》2007,86(6):303-313
Procathepsin D (pCD), the precursor form of lysosomal aspartic protease, is overexpressed and secreted by various carcinomas. The fact that secreted pCD plays an essential role in progression of cancer has been established. In this study, we describe substantial secretion of pCD by the human keratinocyte cell line HaCaT, under serum-free conditions. Moreover, exogenous addition of purified pCD enhanced the proliferation of HaCaT cells. The proliferative effect of pCD was inhibited by a monoclonal antibody against the activation peptide (AP) of pCD. Treatment of HaCaT cells with pCD or AP led to the secretion of a set of cytokines that might promote the growth of cells in a paracrine manner. The role of secreted pCD and its mechanism of action were studied in a scratch wound model and the presence of pCD and AP enhanced regeneration, while this effect was reversed by the addition of anti-AP antibody. Expression and secretion of pCD was upregulated in HaCaT cells exposed to various stress conditions. Taken together, our results strongly suggest that the secretion of pCD is not only linked to cancer cells but also plays a role in normal physiological conditions like wound healing and tissue remodeling. 相似文献
60.
Vasquez-Martinez Y Ohri RV Kenyon V Holman TR Sepúlveda-Boza S 《Bioorganic & medicinal chemistry》2007,15(23):7408-7425
Human lipoxygenase (hLO) isozymes have been implicated in a number of disease states and have attracted much attention with respect to their inhibition. One class of inhibitors, the flavonoids, have been shown to be potent lipoxygenase inhibitors but their study has been restricted to those compounds found in nature, which have limited structural variability. We have therefore carried out a comprehensive study to determine the structural requirements for flavonoid potency and selectivity against platelet 12-hLO, reticulocyte 15-hLO-1, and prostate epithelial 15-hLO-2. We conclude from this study that catechols are essential for high potency, that isoflavones and isoflavonones tend to select against 12-hLO, that isoflavons tend to select against 15-hLO-1, but few flavonoids target 15-hLO-2. 相似文献