全文获取类型
收费全文 | 327篇 |
免费 | 18篇 |
出版年
2021年 | 1篇 |
2020年 | 1篇 |
2019年 | 1篇 |
2018年 | 5篇 |
2017年 | 5篇 |
2016年 | 9篇 |
2015年 | 6篇 |
2014年 | 10篇 |
2013年 | 20篇 |
2012年 | 21篇 |
2011年 | 19篇 |
2010年 | 8篇 |
2009年 | 5篇 |
2008年 | 10篇 |
2007年 | 20篇 |
2006年 | 17篇 |
2005年 | 15篇 |
2004年 | 14篇 |
2003年 | 23篇 |
2002年 | 21篇 |
2001年 | 5篇 |
2000年 | 3篇 |
1999年 | 8篇 |
1998年 | 6篇 |
1997年 | 13篇 |
1996年 | 6篇 |
1995年 | 9篇 |
1994年 | 4篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1986年 | 3篇 |
1985年 | 2篇 |
1984年 | 6篇 |
1983年 | 4篇 |
1982年 | 7篇 |
1981年 | 8篇 |
1980年 | 4篇 |
1979年 | 1篇 |
1978年 | 3篇 |
1976年 | 4篇 |
1975年 | 2篇 |
1974年 | 3篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1967年 | 4篇 |
1965年 | 1篇 |
排序方式: 共有345条查询结果,搜索用时 15 毫秒
61.
Anzai N Miyazaki H Noshiro R Khamdang S Chairoungdua A Shin HJ Enomoto A Sakamoto S Hirata T Tomita K Kanai Y Endou H 《The Journal of biological chemistry》2004,279(44):45942-45950
The urate-anion exchanger URAT1 is a member of the organic anion transporter (OAT) family that regulates blood urate level in humans and is targeted by uricosuric and antiuricosuric agents. URAT1 is expressed only in the kidney, where it is thought to participate in tubular urate reabsorption. We found that the multivalent PDZ (PSD-95, Drosophila discs-large protein, Zonula occludens protein 1) domain-containing protein, PDZK1 interacts with URAT1 in a yeast two-hybrid screen. Such an interaction requires the PDZ motif of URAT1 in its extreme intracellular C-terminal region and the first, second, and fourth PDZ domains of PDZK1 as identified by yeast two-hybrid assay, in vitro binding assay and surface plasmon resonance analysis (K(D) = 1.97-514 nM). Coimmunoprecipitation studies revealed that the wild-type URAT1, but not its mutant lacking the PDZ-motif, directly interacts with PDZK1. Colocalization of URAT1 and PDZK1 was observed at the apical membrane of renal proximal tubular cells. The association of URAT1 with PDZK1 enhanced urate transport activities in HEK293 cells (1.4-fold), and the deletion of the URAT1 C-terminal PDZ motif abolished this effect. The augmentation of the transport activity was accompanied by a significant increase in the V(max) of urate transport via URAT1 and was associated with the increased surface expression level of URAT1 protein from HEK293 cells stably expressing URAT1 transfected with PDZK1. Taken together, the present study indicates the novel role of PDZK1 in regulating the functional activity of URAT1-mediated urate transport in the apical membrane of renal proximal tubules. 相似文献
62.
Nagase Takahiro; Seki Naohiko; Tanaka Ayako; Ishikawa Ken-ichi; Nomura Nobuo 《DNA research》1995,2(4):167-174
In this series of projects regarding the accumulation of sequenceinformation of unidentified human genes, we newly deduced thesequences of 40 full-length cDNA clones of human cell line KG-1,and predicted the coding sequences of the corresponding genes,named KIAA0121 to 0160. The results of a computer search ofpublic databases indicated that the sequences of 13 genes wereunrelated to any reported genes, while the remaining 27 genescarried sequences which showed some similarities to known genes.Obvious unique sequences noted were as follows. A stretch oftriplet repeats was contained in each of three genes: Thesewere GAG(Glu) in KIAA0122 and KIAA0147, and TCC(Ser) in KIAA0150.A stretch of 10 amino acidresidues was repeated 21 times inKIAA0139, and a homologous sequence of 7678 nucleotideswas found repeated 6 times in the untranslated region of KIAA0125.northern hybridization analysis demonstrated that 13 genes wereexpressed in a cell- or tissue-specific manner. Although a vastnumber of expressed sequence tags (ESTs) have been registeredfor comprehensive analysis of cDNA clones, our sequence dataindicated that their distribution is very unbalanced: e.g. whileno EST hit 7 genes, 85 ESTs fell in a single gene. 相似文献
63.
Kosuke Kurosawa Seiji Sugita Ko Ishibashi Sunao Hasegawa Yasuhito Sekine Nanako O. Ogawa Toshihiko Kadono Sohsuke Ohno Naohiko Ohkouchi Yoichi Nagaoka Takafumi Matsui 《Origins of life and evolution of the biosphere》2013,43(3):221-245
Cyanide compounds are amongst the most important molecules of the origin of life. Here, we demonstrate the importance of mid-size (0.1–1 km in diameter) hence frequent meteoritic impacts to the cyanide inventory on the early Earth. Subsequent aerodynamic ablation and chemical reactions with the ambient atmosphere after oblique impacts were investigated by both impact and laser experiments. A polycarbonate projectile and graphite were used as laboratory analogs of meteoritic organic matter. Spectroscopic observations of impact-generated ablation vapors show that laser irradiation to graphite within an N2-rich gas can produce a thermodynamic environment similar to that produced by oblique impacts. Thus, laser ablation was used to investigate the final chemical products after this aerodynamic process. We found that a significant fraction (>0.1 mol%) of the vaporized carbon is converted to HCN and cyanide condensates, even when the ambient gas contains as much as a few hundred mbar of CO2. As such, the column density of cyanides after carbon-rich meteoritic impacts with diameters of 600 m would reach ~10 mol/m2 over ~102 km2 under early Earth conditions. Such a temporally and spatially concentrated supply of cyanides may have played an important role in the origin of life. 相似文献
64.
Takuma Watanobe Naotaka Ishiguro Naohiko Okumura Masuo Nakano Akira Matsui Hitomi Hongo Hiroshi Ushiro 《Journal of molecular evolution》2001,52(3):281-289
The Kabukai A site (5 to 8C A.D.) of the Okhotsk cultural area is on Rebun Island, a small island near the coast, north–northwest
of Hokkaido, Japan. Specimens of Sus scrofa, called the Sakhalin pig, were discovered in five cultural layers at the Kabukai A site. Ancient DNA was extracted from the
remains of 42 Sakhalin pig bones. Thirty-nine nucleotide sequences of the 574-bp mitochondrial DNA control region, estimated
to have originated from at least 21 individuals, were amplified and analyzed phylogenetically. Nine distinct haplotypes (A1,
A2, A3, B1, B2, C1, C2, D1, and D2) from this site were classified into four haplotype groups (A, B, C, and D) by parsimonious
network analysis. Phylogenetic analysis of 9 ancient and 55 modern haplotypes indicated that the population of Sakhalin pigs
at the Kabukai A site belonged to two distinct clusters; haplotype groups A and B formed a cluster comprised only of themselves,
and haplotype groups C and D belonged to the cluster of one of the two genetic groups of Japanese wild boars uniquely distributed
in the western part of Japan, including one northeast Mongolian wild boar. Analysis of the haplotype distribution among three
archaeological sites and their historical transitions among the five layers reflecting the cultural periods at the Kabukai
A site suggests that the Sakhalin pig populations were introduced from Sakhalin island and the Amur River basin in the northeastern
Eurasian continent together with some cultural influences.
Received: 18 April 2000 / Accepted: 24 November 2000 相似文献
65.
Cheek-pouch dispersal of seeds by Japanese monkeys (Macaca fuscata yakui) on Yakushima Island, Japan
Seed dispersal by Japanese monkeys (Macaca fuscata yakui) via cheek-pouch was studied in a warm temperate evergreen forest on Yakushima Island. Plant list was compiled based on a
study during 1986–1995, of which troops of monkeys have been habituated without artificial feeding. We followed the well-habituated
monkeys in 1993 and 1994 to observe the feeding behavior and their treatments of fruits and seeds, and collected seeds dispersed
by monkeys to record the distance carried from the mother trees. We checked the difference of germination ratio between seeds
dispersed via cheek-pouch and seeds taken from mother trees by sowing experiments. Seeds and acorns of 22 species were observed
to be dispersed via cheek-pouch of monkeys. Among them, three species with acorns were never dispersed via feces, and 15 species
with drupes were seldom dispersed via feces. Plant species of which seeds are dispersed only via cheek-pouch had larger seeds
than those of dispersed both via cheek-pouch and via feces, and typically had only one or two seeds in a fruit. As for one
of cheek-pouch dispersal species,Persea thunbergii, the mean distance when seeds were carried from the mother trees via cheek-pouch was 19.7 m, and the maximum distance was
as long as 105 m although more than 80% of seeds were dispersed within 30 m from mother trees. And 82% of seeds dispersed
via cheek-pouch germinated. The easy separation of seeds from other parts of the fruit seems to facilitate cheek-pouch dispersal
more than dispersal via feces. Cheek-pouch dispersal by monkeys has possibly enhanced the natural selection for larger seeds
which bring forth larger seedlings with high shade-tolerance. In conclusion, cheek-pouch dispersal by monkeys is quite an
important mode for trees in the mature stand in a warm temperate evergreen forest on Yakushima Island. 相似文献
66.
Shuntaro Watanabe Koh-Ichi Takakura Yuko Kaneko Naohiko Noma Takayoshi Nishida 《Journal of plant research》2018,131(4):623-631
Heterodichogamy is defined as the presence of two flower morphs that exhibit the male and female functions at different times among individuals within a population. Heterodichogamy is regarded as an adaptation to promote outcrossing through enhanced inter-morph mating, together with a 1:1 morph ratio. However, in highly fragmented populations, the morph ratio may be more likely to be biased by stochastic events. In such a situation, individuals of a minority morph within a population are expected to have higher reproductive success than those of a majority morph, which may suffer from pollen shortages of the minority morph. In this paper, we evaluated mating patterns and male reproductive success in a highly fragmented population of Machilus thunbergii, a putative heterodichogamous evergreen laurel tree. Results of paternity analysis indicated that the selfing rate was not clearly different between the two morphs. In contrast, the proportion of intra-morph mating was higher in the majority-morph (MM) mother trees than in the minority-morph (MF) mother trees. Bayesian estimated male reproductive success indicated that male reproductive success was higher in minority-morph (MF) than in majority-morph (MM) mother trees. These findings indicate that (1) the majority morph mothers, suffering a shortage of the opposite morph pollen, could partly compensate for the reduced reproductive success by intra-morph mating rather than by selfing, and (2) negative-frequency dependent selection may be involved in the maintenance of the two morphs. 相似文献
67.
Tomoko Eguchi-Ogawa Tatsuya Matsubara Daisuke Toki Naohiko Okumura Asako Ando Hitoshi Kitagawa 《Animal biotechnology》2018,29(3):227-233
Widely used antipig CD4 monoclonal antibodies (mAbs) fail to recognize CD4 alleles characteristic of miniature pig lines such as the National Institutes of Health (NIH) miniature pigs and microminipigs. We surveyed polymorphisms in the coding sequence of the porcine CD4 gene among Western and Oriental pig breeds and Japanese wild boars and investigated their distribution. Of the 13 alleles that we identified among the 47 animals, 2 in group I and 3 in group II were found exclusively in Western breed pigs. Group IV alleles, which included mAb-nonbinding alleles, were found frequently in Oriental breed pigs, suggesting that the mAb-nonbinding allele arose from the gene pool of Oriental pigs. Group IV alleles were also found in Duroc and Large White pigs, suggesting genetic inflow from Oriental pig breeds into Western breeds. Comparison of the CD4 sequences of species in Cetartiodactyla suggested that the group IV alleles in Sus scrofa occurred before the divergence of this species from the other artiodactyls. The different antibody specificities of the various CD4 alleles may facilitate the discrimination of T-cell populations in transplantation studies using miniature pigs. The significance of the preservation of CD4 polymorphisms to immune function in pigs warrants further investigation. 相似文献
68.
Naohiko Okumura Naotaka Ishiguro Masuo Nakano Katsuya Hirai Akira Matsui Makoto Sahara 《Biochemical genetics》1996,34(5-6):179-189
Mitochondrial DNA (mtDNA) control regions from 40 Japanese wild boars were examined by direct sequencing after amplification
by PCR. From the DNA sequences obtained, we found eight haplotypes, whose differences arose via transitions. The geographical
distribution of these different haplotypes indicated that wild boar populations inhabited limited areas and that there was
some restricted gene flow between local populations. Eight mtDNA haplotypes from Eastern and Western domestic pigs and the
Ryukyu wild boar were also analyzed as references to those from Japanese wild boars. The cluster analyses of the control-region
sequences showed that those from Japanese wild boards belong to the Asian type as do those from Eastern domestic pigs and
the Ryukyu wild boar, which differed from the European type (Western domestic pigs). 相似文献
69.
Nagase Takahiro; Miyajima Nobuyuki; Tanaka Ayako; Sazuka Takashi; Seki Naohiko; Sato Shusei; Tabata Satoshi; Ishikawa Ken-ichi; Kawarabayasi Yutaka; Kotani Hirokazu; Nomura Nobuo 《DNA research》1995,2(1):37-43
We isolated full-length cDNA clones from size-fractionated cDNAlibraries of human immature myeloid cell line KG-1, and thecoding sequences of 40 genes were newly predicted. A computersearch of the GenBank/EMBL databases indicated that the sequencesof 14 genes were unrelated to any reported genes, while theremaining 26 genes carried some sequences with similaritiesto known genes. Significant transmembrane domains were identifiedin 17 genes, and protein motifs that matched those in the PROSITEmotif database were identified in 11 genes. Northern hybridizationanalysis with 18 different cells and tissues demonstrated that10 genes were apparently expressed in a cell-specific or tissue-specificmanner. Among the genes predicted, half were isolated from themedium-sized cDNA library and the other half from the small-sizedcDNA library, and their average sizes were 4 kb and 1.4 kb,respectively. As judged by Northern hybridization profiles,small-sized cDNAs appeared to be expressed more ubiquitouslyand abundantly in various tissues, compared with that of medium-sizedcDNAs. 相似文献
70.
Yasumitsu Hidetaro; Shofuda Ken-ich; Nishihashi Ai; Eki Toshihiko; Koshikawa Naohiko; Mizushima Hiroto; Miyazaki Kaoru 《DNA research》1997,4(1):77-79
A new group of matrixmetalloproteinase with a potential membranedomain was reported as membranetype matrixmetalloproteinases(MT-MMPs), and the gene coding for one of them, MT2-MMP, wasrecently cloned from a human lung cDNA library. To predict itsphysiological functions by the relation to the genetic disordersmapped on the chromosomes, the chromosomal location of the humanMT2-MMP gene was examined by fluorescence in situ hybridization(FISH) and PCR-based analysis with human/rodent hybrid cellmapping panels, and it was assigned to 16q12. 相似文献