全文获取类型
收费全文 | 266篇 |
免费 | 3篇 |
出版年
2023年 | 1篇 |
2021年 | 4篇 |
2019年 | 1篇 |
2018年 | 2篇 |
2017年 | 1篇 |
2016年 | 4篇 |
2015年 | 3篇 |
2014年 | 8篇 |
2013年 | 14篇 |
2012年 | 11篇 |
2011年 | 10篇 |
2010年 | 5篇 |
2009年 | 6篇 |
2008年 | 9篇 |
2007年 | 13篇 |
2006年 | 14篇 |
2005年 | 14篇 |
2004年 | 16篇 |
2003年 | 10篇 |
2002年 | 18篇 |
2001年 | 9篇 |
2000年 | 8篇 |
1999年 | 12篇 |
1998年 | 4篇 |
1997年 | 7篇 |
1996年 | 8篇 |
1995年 | 4篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1991年 | 4篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 3篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1980年 | 3篇 |
1979年 | 4篇 |
1978年 | 2篇 |
1977年 | 3篇 |
1976年 | 4篇 |
1975年 | 1篇 |
1973年 | 1篇 |
1972年 | 1篇 |
排序方式: 共有269条查询结果,搜索用时 15 毫秒
151.
Tsutsui N Ohira T Kawazoe I Takahashi A Wilder MN 《Marine biotechnology (New York, N.Y.)》2007,9(3):360-369
Vitellogenesis-inhibiting hormone (VIH) in Crustacea belongs to the crustacean hyperglycemic hormone (CHH)-family. To characterize
multiple VIH molecules in the whiteleg shrimp Litopenaeus vannamei, seven CHH-family peptides designated as Liv-SGP-A, -B,
-C, -D, -E, -F, and -G were purified by reversed-phase HPLC and identified by N-terminal amino acid sequencing. The dose-response
effects of these peptides on vitellogenin mRNA levels were examined using in vitro incubation of ovarian fragments of the
kuruma prawn Marsupenaeus japonicus. Liv-SGP-D showed no significant inhibitory activities, while the other six peptides significantly
reduced vitellogenin mRNA levels, however, with differing efficacies, in the order of Liv-SGP-C, -F, -G > -A, -B > -E. Liv-SGP-G
was the most abundant CHH-family peptide in the sinus gland and showed strong vitellogenesis-inhibiting activity. As a result
of detailed structural analysis, its complete primary structure was determined; it consisted of 72 amino acid residues and
possesses an amidated C-terminus.
Tsutsui and Ohira contributed equally to this work. 相似文献
152.
Characterization and cDNA cloning of androgenic gland hormone of the terrestrial isopod Armadillidium vulgare. 总被引:1,自引:0,他引:1
A Okuno Y Hasegawa T Ohira Y Katakura H Nagasawa 《Biochemical and biophysical research communications》1999,264(2):419-423
The sex differentiation in crustaceans is known to be controlled by a peptide hormone called androgenic gland hormone (AGH). AGH was extracted and purified from the androgenic glands (AGs) of the male isopod Armadillidium vulgare by high-performance liquid chromatography. AGH consisted of two peptide chains and their N-terminal amino acid sequences were determined. A cDNA encoding AGH was cloned by PCR and sequenced. The cDNA had an open reading frame of 432 bp, which encoded a preproAGH consisting of a signal peptide (21 residues), B chain (44 residues), C peptide (46 residues), and A chain (29 residues). Through processing, the A and B chains might form a heterodimer interlinked by disulfide bonds. The A chain possessed a putative N-linked glycosylation site. A Northern blot analysis using the cDNA as a probe detected a hybridization signal with 0.8 kb in the RNA preparation only from the AGs. 相似文献
153.
154.
Expression of a recombinant molt-inhibiting hormone of the kuruma prawn Penaeus japonicus in Escherichia coli. 总被引:1,自引:0,他引:1
T Ohira T Nishimura H Sonobe A Okuno T Watanabe H Nagasawa I Kawazoe K Aida 《Bioscience, biotechnology, and biochemistry》1999,63(9):1576-1581
The crustacean molt-inhibiting hormone (MIH) suppresses ecdysteroid synthesis by the Y-organ. The MIH of the kuruma prawn Penaeus japonicus has recently been isolated and its cDNA cloned. In this study, we expressed the MIH in Escherichia coli to obtain a large quantity of this hormone with biological activity. The MIH cDNA was processed and ligated into an expression plasmid. E. coli was transformed with this plasmid, and then the recombinant MIH (r-MIH) was expressed. The r-MIH was put through the refolding reaction and was purified by reverse-phase HPLC. N-terminal amino acid sequence and time-of-flight mass spectral analyses supported the idea that the r-MIH had the entire sequence. By in vitro bioassay using the Y-organ of the crayfish, the r-MIH was found to be comparable to natural MIH in inhibiting ecdysteroid synthesis. 相似文献
155.
Variations in the Contents and Kinetic Properties of Ribulose-1,5-bisphosphate Carboxylases among Rice Species 总被引:5,自引:0,他引:5
The Km(CO2) ancl Vmax of ribulose 1,5-bisphosphate (RuBP) carboxylaseand its protein ratio to total soluble protein from Oryza speciesincluding cultivars (25 varieties) and wild types (11 species,21 strains) were surveyed. Their variabilities among cultivarsof O. sativa were very small. The averages of the Km(CO2) andVmax values and the ratio of carboxylase to soluble protein,and their standard errors were 10.2?1.0µM, 1.72?0.13units.mg1(pH 8.0 and 25?C) and 52?2%, respectively. However, some differencesseemed to exist based on genome constitution in the Oryza genus.RuBP carboxylases from the species with the AgAg genome, O.graberrima and O. breviligulate, exhibited low Km(CO2) values(8.0?0.8 µM). High Vmax was associated with the CC genome,O. eichingeri and O. officinalis (2.08?0.15 units.mg1).A higher ratio of RuBP carboxylase protein to soluble proteinwas found for the AA genome, O. sativa and O. perennis. (Received September 24, 1986; Accepted April 15, 1987) 相似文献
156.
Takahiro Ohkubo Koh Kidena Naohiko Takimoto Akihiro Ohira 《Journal of molecular modeling》2011,17(4):739-755
We measured the water uptakes and proton conductivities of a Nafion membrane and three sulfonated polyether sulfone membranes
(SPESs) with different values of ion-exchange capacity (IEC = 0.75, 1.0 and 1.4 meq/g) in relation to relative humidity in
order to apply the findings to polymer electrolyte membrane fuel cells. The number of water molecules per sulfonic acid group
λ at each humidity level was independent of the relative humidity for all membranes, but the proton conductivities of the SPESs
were inferior to that of Nafion for the same λ value. Classical molecular dynamics simulations for the same membranes were carried out using a consistent force field at
λ = 3, 6, 9, 12 and 15. The structural properties of water molecules and hydronium ions at a molecular level were estimated
from radial distribution functions and cluster size distributions of water. We found that the radial distribution function
of S(sulfonic acid)–S(sulfonic acid) of Nafion at λ = 3 indicated a significant correlation between the S–S pair, due to water channels, while the S–S pair of the SPESs showed
a poor correlation. The cluster size distribution of water was also calculated in order to estimate the connectivity of the
water channel. It is clear that some water is present in the SPESs as small, isolated clusters, especially when the water
content is low. 相似文献
157.
158.
159.
An unknown ninhydrin-positive compound was detected on a paperchromatogram of the free amino acid fraction prepared from greenleaf blades of rice plant (Oryza saliva Norin 16). This compoundwas identified as D-alanylglycine. (Received November 24, 1978; ) 相似文献
160.
Relation between Leaf Age and Nitrogen Incorporation in the Leaf of the Rice Plant (Oryza sativa L.)
The relation between leaf age and the incorporation of nitrogeninto the leaf was examined in the 13th leaf blade of the riceplant, Oryza sativa L., after feeding it 15N-labelled ammoniumsulfate. The incorporation of nitrogen into the leaf was activeup to the maximum leaf nitrogen content; thereafter it decreasedquickly. At the beginning of senescence, when the nitrogen contentof the leaf had begun to decrease, the incorporation of 15Ndecreased to 28% of the value during development. At the middlestage of senescence, when the nitrogen content had decreasedto half the maximum, the incorporation was 13%. The incorporation of 15N into soluble proteins was examinedby gel filtration on Sephadex G-200. During development, largeamounts of 15N were incorporated into the ribulose bisphosphatecarboxylase-rich fraction. As the leaf aged, the incroporationof 15N into this fraction decreased more sharply than it didin other fractions. This tendency was more pronounced at thelate stage of senescence. We concluded that the amounts andkinds of protein synthesized in a leaf are closely related toleaf age. (Received November 24, 1981; Accepted June 17, 1982) 相似文献