全文获取类型
收费全文 | 8233篇 |
免费 | 861篇 |
国内免费 | 2篇 |
专业分类
9096篇 |
出版年
2021年 | 114篇 |
2020年 | 70篇 |
2019年 | 96篇 |
2018年 | 108篇 |
2017年 | 99篇 |
2016年 | 187篇 |
2015年 | 276篇 |
2014年 | 342篇 |
2013年 | 413篇 |
2012年 | 457篇 |
2011年 | 494篇 |
2010年 | 310篇 |
2009年 | 301篇 |
2008年 | 412篇 |
2007年 | 430篇 |
2006年 | 379篇 |
2005年 | 350篇 |
2004年 | 374篇 |
2003年 | 345篇 |
2002年 | 346篇 |
2001年 | 164篇 |
2000年 | 163篇 |
1999年 | 164篇 |
1998年 | 122篇 |
1997年 | 103篇 |
1996年 | 111篇 |
1995年 | 86篇 |
1994年 | 82篇 |
1993年 | 92篇 |
1992年 | 124篇 |
1991年 | 118篇 |
1990年 | 115篇 |
1989年 | 83篇 |
1988年 | 82篇 |
1987年 | 86篇 |
1986年 | 71篇 |
1985年 | 91篇 |
1984年 | 83篇 |
1983年 | 78篇 |
1982年 | 65篇 |
1981年 | 74篇 |
1980年 | 57篇 |
1979年 | 51篇 |
1978年 | 54篇 |
1977年 | 46篇 |
1976年 | 52篇 |
1975年 | 46篇 |
1974年 | 48篇 |
1973年 | 50篇 |
1971年 | 43篇 |
排序方式: 共有9096条查询结果,搜索用时 0 毫秒
91.
92.
Terry W. Spithill Raelene J. Grumont Graham F. Mitchell 《Journal of cellular biochemistry》1984,24(2):103-112
The genetic characterization of pathogenic isolates of Leishmania was attempted by analysis of the molecular properties of kinetoplast DNA (kDNA) minicircles. Unit minicircle size is not conserved during speciation of Leishmania since the minicircles of strains and clones of L t major are smaller (700 bp) than those found in certain strains of L mexicana ssp (820 bp), L donovani (850 bp) or L t tropica (900 bp). Schizodeme analysis of minicircles reveals a high degree of sequence divergence in kDNA of Leishmania with the degree of microheterogeneity varying between species. This sequence divergence allows the discrimination of species, strains, and clones of Leishmania into schizodemes. Southern blot hybridization experiments reveal that at high stringency overall minicircle sequence homology is conserved among clones and strains of one species (L t major) but not between different species. This property of minicircle DNA permits the use of kDNA probes as a species-specific diagnostic test for the identification of unknown Leishmania isolates. The properties of kDNA from an L t tropica strain LRC-L32 (a “recidiva” organism) are so diverged from those of L t major strains as to support the classification [22,23] of L t tropica and L t major as separate species of Leishmania rather than subspecies of L tropica. 相似文献
93.
94.
R M Siervogel R C Elston R H Lester J B Graham 《American journal of human genetics》1979,31(2):199-213
Blood clotting factor ten (X) levels measured in 149 people in six pedigrees were found to fit a mixture of normal distributions. No environmental effect could be identified to account for the wide separation in the means of these distributions. Pedigree analysis reveals that the data are compatible with an autosomal, one locus, two allele genetic model affecting factor X activity. Goodness of fit tests suggest that the allele for low levels of factor X is dominant, though on the basis of likelihood tests, mean heterozygote levels are different from mean homozygote levels. A similar bimodal distribution for factor X levels observed previously in a separate sample of 207 young men, indicated that the proposed dominant allele has an estimated population gene frequency of .53. The earlier estimate is remarkably similar to that obtained with the currently ascertained pedigrees. The postulated major gene accounts for more than half of the variation in factor X levels. 相似文献
95.
Graham S. Baldwin Alphonse Galdes H.Allen O. Hill Stephen G. Waley Edward P. Abraham 《Journal of inorganic biochemistry》1980,13(3):189-204
β-Lactamase II has two metal-binding sites. The electronic spectra of Cd(II)- and Co(II)-substituted β-lactamase II have been investigated. It is suggested that a thiol ligand is involved in metal binding at the first site. The stoichiometric dissociation constants for Co(II) binding to β-lactamase II were estimated to be 0.13 and 2.66 mM (pH 6.0, 4°C, 1 M NaCl) by equilibrium dialysis. Competition between Zn(II) and Co(II) for the first metal binding site suggests a value of 0.7 μM (pH 6.0, 30°C, 1 M NaCl) for the dissociation constant o Zn(II).The electronic spectra of the Co(II) enzyme lead to the suggestion that the coordination geometries around the metal ions in the first and second sites are related to those of a distorted tetrahedron and octahedron, respectively. 相似文献
96.
Affinity chromatography has been used to isolate and compare the peanut agglutinin receptors from neuraminidase-treated human, bovine and porcine erythrocyte membranes. Passage of Triton X-100-solubilised membrane material through either Sepharose- or acrylamide-based affinity columns resulted in the reversible binding of receptor molecules to the immobilised lectin. Elution with 0.2M galactose released specifically bound glycoprotein fractions, the composition and molecular weights of which were determined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate.Carbohydrate analysis by gas chromatography identified these bound glycoprotein fractions as the major sources of the disaccharide in these membranes. It is suggested that these isolated fractions represent a discrete population of glycoproteins within the membranes studied, which possess both - and carbohydrates. 相似文献
97.
98.
In a study to assess the natural history of giant-cell arteritis, 90 patients with proved disease were followed up from the time of diagnosis. Early mortality was low and most commonly due to vertebral arteritis, but cerebral infarction did not appear to be a late complication. High maintenance dose steroids and visual loss were associated significantly with a shortened life span (p=0.0003 and p=0.0024). One-third of the patients developed chronic relapsing disease, but serious late complications were not encountered. After the initial attack has been controlled steroid dosage should be reduced to the minimum needed to alleviate symptoms. 相似文献
99.
Graham K. Moore George A.F. Roberts 《International journal of biological macromolecules》1981,3(5):292-296
A study has been made of the influence of reaction medium on the N-acetylation of chitosan under heterogeneous conditions. The results show that provided a pre-steeping treatment is given a range of reaction media permit rapid N-acetylation. The influence of the nature of the N-acyl group on O-acetylation has also been studied. In general the larger the N-acyl group the greater the ease of O-acetylation, although too bulky a group inhibits reaction through steric hindrance. In all cases the rate of O-acetylation falls to nearly zero when ~ 50% of the hydroxyl groups have reacted, and prolonged reaction times are required if a more highly acetylated product is required. 相似文献
100.