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71.
A new species of Tzeltalia (Solanaceae) from Chiapas, Mexico is described and illustrated: Tzeltalia esenbeckii. A key and a table, distinguishing the members of the genus, are included. 相似文献
72.
Alexander-Aguilera A Berruezo S Hernández-Diaz G Angulo O Oliart-Ros R 《Journal of physiology and biochemistry》2011,67(4):595-604
The fatty acid profile of hepatocytes and adipocytes is determined by the composition of the dietary lipids. It remains unclear which fatty acid components contribute to the development or reduction of insulin resistance. The present work examined the fatty acid composition of both tissues in sucrose-induced obese rats receiving fish oil to determine whether the effect of dietary (n-3) polyunsaturated fatty acids (PUFAs) on the reversion of metabolic syndrome in these rats is associated to changes in the fatty acid composition of hepatocyte and adipocyte membrane lipids. Animals with metabolic syndrome were divided into a corn–canola oil diet group and a fish oil diet group, and tissues fatty acids composition were analyzed after 6 weeks of dietary treatment. Fatty acid profiles of the total membrane lipids were modified by the fatty acid composition of the diets fed to rats. N-3 PUFAs levels in animals receiving the fish oil diet plus sucrose in drinking water were significantly higher than in animals under corn–canola oil diets. It is concluded that in sucrose-induced obese rats, consumption of dietary fish oil had beneficial effects on the metabolic syndrome and that such effects would be conditioned by the changes in the n-3 PUFAs composition in hepatic and adipose tissues because they alter membrane properties and modify the type of substrates available for the production of active lipid metabolites acting on insulin resistance and obesity. 相似文献
73.
David Requena Ana Chumbe Michael Torres Ofelia Alzamora Manuel Ramirez Hugo Valdivia-Olarte Andres Hazaet Gutierrez Ray Izquierdo-Lara Luis Enrique Saravia Milagros Zavaleta Luis Tataje-Lavanda Ivan Best Manolo Fernández-Sánchez Eliana Icochea Mirko Zimic Manolo Fernández-Díaz 《Bioinformation》2013,9(10):528-536
Background: Avibacterium paragallinarum, the causative agent of infectious coryza, is a highly
contagious respiratory acute disease of poultry, which affects commercial chickens, laying hens and broilers worldwide.
Methodology: In this study, we performed the whole genome sequencing, assembly and annotation of a Peruvian
isolate of A. paragallinarum. Genome was sequenced in a 454 GS FLX Titanium system. De novo assembly was
performed and annotation was completed with GS De Novo Assembler 2.6 using the H. influenzae str. F3031 gene model.
Manual curation of the genome was performed with Artemis. Putative function of genes was predicted with Blast2GO.
Virulence factors were identified by comparison with the Virulence Factor Database.
Results: The genome obtained has a length of 2.47 Mb with 40.66% of GC content. Seventy five large contigs
(>500 nt) were obtained, which comprised 1,204 predicted genes. All the contigs are available in Genbank
[GenBank: PRJNA64665]. A total of 103 virulence factors, reported in the Virulence Factor Database, were
found in A. paragallinarum. Forty four of them are present in 7 species of Haemophilus, which are related
with pathogenesis, virulence and host immune system evasion. A tetracycline-resistance associated transposon
(Tn10), was found in A. paragallinarum, possibly acting as a defense mechanism.
Discussion and conclusion: The availability of A. paragallinarum genome represents an important source of information for
the development of diagnostic tests, genotyping, and novel antigens for potential vaccines against infectious coryza.
Identification of virulence factors contributes to better understanding the pathogenesis, and planning efforts for prevention
and control of the disease. 相似文献
74.
Histological development of the long‐snouted seahorse Hippocampus guttulatus during ontogeny
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Claudia Ofelio Alcira Ofelia Díaz Giuseppe Radaelli Miquel Planas 《Journal of fish biology》2018,93(1):72-87
The objective of the present study was to describe histological development of the European long‐snouted seahorse Hippocampus guttulatus, to increase understanding of the biology and physiology of the species. Most vital organs were present in juveniles by the time of their release from the male's pouch. Digestive tract specialization occurred at 89 effective day‐degrees (D°eff), corresponding to 15 days post partum (dpp), with development of the first intestinal loop and mucosal folding. At 118 D°eff (20 dpp), lipids were being mobilized from the liver and oocytes attained the perinuclear stage. The fovea emerged at 177 D°eff (30 dpp), contemporaneous with the shift from pelagic to benthic behaviour in juveniles. At this stage, the most interesting feature was the formation of the second intestinal loop. Male gonads were never observed during the study (from 0 to 354 D°eff; 0–60 dpp), but the first oogonia were present at 30 D°eff (5 dpp). In 354 D°eff (60 dpp) juveniles, oocytes were observed in a cortical alveoli stage, indicating maturity. Low digestive efficiency was observed at early stages, which was due to a poorly developed gastrointestinal tract and an immature digestive tract prior to 89 D°eff. The present study demonstrates that approximately 89 and 177 D°eff represent two important transitional stages in the early development of H. guttulatus. At a temperature of approximately 19 ± 1°C and an age of 1 month (177 D°eff), main organs were fully functional, suggesting that the adult phenotype was largely established by that age, with females becoming mature at the age of 2 months (354 D°eff). 相似文献
75.
Jesse A. Port James L. O'Donnell Ofelia C. Romero‐Maraccini Paul R. Leary Steven Y. Litvin Kerry J. Nickols Kevan M. Yamahara Ryan P. Kelly 《Molecular ecology》2016,25(2):527-541
Preserving biodiversity is a global challenge requiring data on species’ distribution and abundance over large geographic and temporal scales. However, traditional methods to survey mobile species’ distribution and abundance in marine environments are often inefficient, environmentally destructive, or resource‐intensive. Metabarcoding of environmental DNA (eDNA) offers a new means to assess biodiversity and on much larger scales, but adoption of this approach for surveying whole animal communities in large, dynamic aquatic systems has been slowed by significant unknowns surrounding error rates of detection and relevant spatial resolution of eDNA surveys. Here, we report the results of a 2.5 km eDNA transect surveying the vertebrate fauna present along a gradation of diverse marine habitats associated with a kelp forest ecosystem. Using PCR primers that target the mitochondrial 12S rRNA gene of marine fishes and mammals, we generated eDNA sequence data and compared it to simultaneous visual dive surveys. We find spatial concordance between individual species’ eDNA and visual survey trends, and that eDNA is able to distinguish vertebrate community assemblages from habitats separated by as little as ~60 m. eDNA reliably detected vertebrates with low false‐negative error rates (1/12 taxa) when compared to the surveys, and revealed cryptic species known to occupy the habitats but overlooked by visual methods. This study also presents an explicit accounting of false negatives and positives in metabarcoding data, which illustrate the influence of gene marker selection, replication, contamination, biases impacting eDNA count data and ecology of target species on eDNA detection rates in an open ecosystem. 相似文献
76.
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78.
Afshin Hosseini Muhammad Rizwan Tariq Fernanda Trindade da Rosa Julia Kesser Zeeshan Iqbal Ofelia Mora Helga Sauerwein James K. Drackley Erminio Trevisi Juan J. Loor 《PloS one》2015,10(11)
The effects of dietary energy level and 2,4-thiazolidinedione (TZD) injection on feed intake, body fatness, blood biomarkers and TZD concentrations, genes related to insulin sensitivity in adipose tissue (AT) and skeletal muscle, and peroxisome proliferator-activated receptor gamma (PPARG) protein in subcutaneous AT (SAT) were evaluated in Holstein cows. Fourteen nonpregnant nonlactating cows were fed a control low-energy (CON, 1.30 Mcal/kg) diet to meet 100% of estimated nutrient requirements for 3 weeks, after which half of the cows were assigned to a higher-energy diet (OVE, 1.60 Mcal/kg) and half of the cows continued on CON for 6 weeks. All cows received an intravenous injection of TZD starting 2 weeks after initiation of dietary treatments and for an additional 2 weeks, which served as the washout period. Cows fed OVE had greater energy intake and body mass than CON, and TZD had no effect during the administration period. The OVE cows had greater TZD clearance rate than CON cows. The lower concentration of nonesterified fatty acids (NEFA) and greater concentration of insulin in blood of OVE cows before TZD injection indicated positive energy balance and higher insulin sensitivity. Administration of TZD increased blood concentrations of glucose, insulin, and beta-hydroxybutyrate (BHBA) at 2 to 4 weeks after diet initiation, while the concentration of NEFA and adiponectin (ADIPOQ) remained unchanged during TZD. The TZD upregulated the mRNA expression of PPARG and its targets FASN and SREBF1 in SAT, but also SUMO1 and UBC9 which encode sumoylation proteins known to down-regulate PPARG expression and curtail adipogenesis. Therefore, a post-translational response to control PPARG gene expression in SAT could be a counteregulatory mechanism to restrain adipogenesis. The OVE cows had greater expression of the insulin sensitivity-related genes IRS1, SLC2A4, INSR, SCD, INSIG1, DGAT2, and ADIPOQ in SAT. In skeletal muscle, where PPARA and its targets orchestrate carbohydrate metabolism and fatty acid oxidation, the OVE cows had greater glyceroneogenesis (higher mRNA expression of PC and PCK1), whereas CON cows had greater glucose transport (SLC2A4). Administration of TZD increased triacylglycerol concentration and altered expression of carbohydrate- and fatty acid oxidation-related genes in skeletal muscle. Results indicate that overfeeding did not affect insulin sensitivity in nonpregnant, nonlactating dairy cows. The bovine PPARG receptor appears TZD-responsive, with its activation potentially leading to greater adipogenesis and lipogenesis in SAT, while differentially regulating glucose homeostasis and fatty acid oxidation in skeletal muscle. Targeting PPARG via dietary nutraceuticals while avoiding excessive fat deposition might improve insulin sensitivity in dairy cows during times such as the peripartal period when the onset of lactation naturally decreases systemic insulin release and sensitivity in tissues such as AT. 相似文献
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80.
The main saponin from the fresh tuber of Dioscorea composita was dioscin and from the fermented material 3-O-[α-l-rhamnopyranosyl(1→4)-β-d-glucopyranosyl]diosgenin. The 13C NMR chemical shifts of saponins were used in the determination of their structure. No free sapogenin was isolated from the fresh tuber. 相似文献