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排序方式: 共有687条查询结果,搜索用时 15 毫秒
681.
682.
Laura A. Elenich D. Nandi A. Elizabeth Kent T. Scott McCluskey M. Cruz Mohan N. Iyer Elaine C. Woodward Christopher W. Conn Amber L. Ochoa David B. Ginsburg J. J. Monaco 《Immunogenetics》1999,49(10):835-842
The proteasome is a large multicatalytic proteinase that plays a role in the generation of peptides for presentation by major
histocompatibility complex class I molecules. The 20S proteolytic core of mammalian proteasomes is assembled from a group
of 17 protein subunits that generate a distinctive pattern of spots upon two-dimensional gel electrophoresis. The genes for
most of these subunits have been cloned from humans and rats. We isolated cDNA clones for the mouse orthologues of ten of
the subunits [PSMA1 (C2), PSMA2 (C3), PSMA3 (C8), PSMA4 (C9), PSMA5 (ZETA), PSMA6 (IOTA), PSMA7 (C6-I), PSMB2 (C7-I), PSMB3
(C10-II), and PSMB5 (X)] to complete the cloning of all of the mouse subunits. Using antisera raised against these subunits
or their orthologues, we verified the identity of these proteins by two-dimensional NEPHGE-PAGE.
Received: 8 March 1999 / Accepted: 8 April 1999 相似文献
683.
Stroma: as an affinity adsorbent for non-inhibitable lectins 总被引:5,自引:0,他引:5
684.
W. C. Johnson L. E. Jackson O. Ochoa R. van Wijk J. Peleman D. A. St. Clair R. W. Michelmore 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(7):1066-1073
Wild plant species are often adapted to more stressful environments than their cultivated relatives. Roots are critical in
exploiting soil resources that enable plants to withstand environmental stresses, but they are difficult to study. Cultivated
lettuce (Lactuca sativa L.) and wild L. serriola L. differ greatly in both shoot and root characteristics. Approximately 100 F2:3 families derived from an interspecific cross were evaluated in greenhouse and field experiments. In the greenhouse, root
traits (taproot length, number of laterals emerging from the taproot, and biomass) and shoot biomass were measured 4 weeks
after planting. In the field, plants were grown for 9 weeks (close to harvest maturity of the cultivated parent); mild drought
stress was induced by withholding water for 1 week, and gravimetric moisture of soil was then determined for five depth increments
between 0–100 cm. The families were genotyped using codominantly scored AFLP markers distributed throughout the genome. Composite
interval mapping was used to analyze marker-trait associations. Quantitative trait loci were identified for differences between
wild and cultivated lettuce for root architectural traits and water acquisition. Thirteen QTL were detected that each accounted
for 28–83% of the phenotypic variation. The loci for taproot length (i.e., cm taproot length g–1 plant biomass) and the ability to extract water from deep in the soil profile co-localized in the genome. These coincident
loci were identified in separate experiments. The wild L. serriola is therefore a potential source of agriculturally important alleles to optimize resource acquisition by cultivated lettuce,
thereby minimizing water and fertilizer inputs and ultimately enhancing water quality.
Received: 25 February 2000 / Accepted: 31 March 2000 相似文献
685.
686.
Ochoa S 《The Biochemical journal》1939,33(8):1262-1270
687.