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排序方式: 共有217条查询结果,搜索用时 31 毫秒
31.
Studies of genetic population structures of clonally reproducing macro-organisms have revealed large areas where only one clone is found. These areas, referred to as clonal patches, have not been shown to occur in free-living microbes until now. In free-living microbes, high genetic diversity at local scales is usually maintained by high rates of dispersal. We report, however, a highly dense, 12-m clonal patch of the social amoeba Dictyostelium discoideum in a cattle pasture located in a Texas Gulf Coast prairie. We confirm the presence of only one clone by the analysis of 65 samples and amplification of 10 polymorphic microsatellite loci. Samplings of additional cattle pastures nearby showed higher clonal diversity, but with a density of D. discoideum isolates lower than in the clonal patch. These findings show that high rates of microbial dispersal do not always produce genetic diversity at local scales, contrary to the findings of previous studies. The existence of clonal patches may be particularly important for microbial social evolution. 相似文献
32.
The effect of cooling rate, freeze-drying suspending fluid and culture age on the preservation of Campylobacter pylori 总被引:1,自引:1,他引:0
The effects of freezing rate, suspending fluid and age of culture on the ability of four strains of Campylobacter pylori to survive and recover from freeze-drying were examined. Freeze-drying by standard procedures generally resulted in an overall loss in viability of between 3 and 7 log units. The exact cause of poor recovery by C. pylori was not established but strain differences were detected, with NCTC 11637 (type strain) surviving better than NCTC 11638 and NCTC 11639. Recovery of the poorest growing strain (NE 26695) was notably more erratic. The largest loss in viability occurred at the primary drying stage. Losses resulting from freezing and secondary drying were less marked and the rate of freezing had only a marginal effect on recovery. Nineteen different freeze-drying suspending fluids were investigated. Overall the best recovery results were obtained with 5% inositol-broth (or horse serum) plus 25% glucose, at pH 7.0, in which loss of viability was typically about 4 log units. Other factors, such as age of culture and number of viable bacteria in the before-dry suspension, did not have a significant effect on survival. We conclude from these results that C. pylori can survive freeze-drying, albeit in small numbers, but the degree of recovery is apparently largely strain dependent. 相似文献
33.
Sampling properties of DNA sequence data in phylogenetic analysis 总被引:20,自引:6,他引:20
We inferred phylogenetic trees from individual genes and random samples of
nucleotides from the mitochondrial genomes of 10 vertebrates and compared
the results to those obtained by analyzing the whole genomes. Individual
genes are poor samples in that they infrequently lead to the whole-genome
tree. A large number of nucleotide sites is needed to exactly determine the
whole-genome tree. A relatively small number of sites, however, often
results in a tree close to the whole-genome tree. We found that blocks of
contiguous sites were less likely to lead to the whole-genome tree than
samples composed of sites drawn individually from throughout the genome.
Samples of contiguous sites are not representative of the entire genome, a
condition that violates a basic assumption of the bootstrap method as it is
applied in phylogenetic studies.
相似文献
34.
JOSEP PENUELAS JORDI SARDANS JOAN LLUSIÀ SUSAN M. OWEN JOFRE CARNICER THOMAS W. GIAMBELLUCA ENRICO L. REZENDE MASHURI WAITE ÜLO NIINEMETS 《Global Change Biology》2010,16(8):2171-2185
Plant‐invasive success is one of the most important current global changes in the biosphere. To understand which factors explain such success, we compared the foliar traits of 41 native and 47 alien‐invasive plant species in Oahu Island (Hawaii), a location with a highly endemic flora that has evolved in isolation and is currently vulnerable to invasions by exotic plant species. Foliar traits, which in most cases presented significant phylogenetic signal, i.e. closely related species tended to resemble each other due to shared ancestry, separated invasive from native species. Invasive species had lower leaf mass per area and enhanced capacities in terms of productivity (photosynthetic capacity) and nutrient capture both of macro‐ (N, P, K) and microelements (Fe, Ni, Cu and Zn). All these differences remain highly significant after removing the effects of phylogenetic history. Alien‐invasive species did not show higher efficiency at using limiting nutrient resources, but they got faster leaf economics returns and occupied a different biogeochemical niche, which helps to explain the success of invasive plants and suggests that potential increases in soil nutrient availability might favor further invasive plant success. 相似文献
35.
ALAN W. OWEN DAVID A.T. HARPER RACHEL A. HEATH 《Lethaia: An International Journal of Palaeontology and Stratigraphy》2008,41(2):173-184
The shelly fauna of the lowest part of the Sælabonn Formation in the Hadeland district of the Oslo Region provides a rare insight into the shallow-water biota of the earliest Silurian and hence the start of biotic recovery after the end Ordovician extinction event. It is dominated by the brachiopods Dalmanella cf. pectinoides Bergström, Coolinia cf. columbana (Reed), Leptaena cf. haverfordensis Bancroft and Zygospiraella scotica (Salter) together with the trilobite Acernaspis elliptifrons (Esmark). The first three species in this unique association belong to eurytopic Ordovician survivor genera which were also present in the underlying unit in Hadeland and continued to thrive during the Silurian. Significantly, Zygospiraella and Acernaspis have no unequivocal Ordovician record but diversified rapidly and became common during the early Silurian (Rhuddanian) in many parts of the world. Moreover, Acernaspis and Zygospiraella were highly eurytopic, pioneer taxa that were among the first elements of the Rhuddanian shelly fauna to appear in many parts of the world especially around the margins of the remnant Iapetus Ocean, following the drowning of areas previously emergent during the late Ordovician sea-level lowstand. The Hadeland fauna provides evidence of a previously undocumented route (among several) by which life ultimately returned to the global diversity plateau established in the mid-Ordovician. 相似文献
36.
SARAH E. STEWART ALAN W. OWEN 《Lethaia: An International Journal of Palaeontology and Stratigraphy》2008,41(2):139-146
A deep‐water Konservat Lagerstätte from the lower Caradoc (Sandbian) at Girvan is dominated by the trilobite Diacanthaspis trippi, the carpoids Anatifopsis n. sp.? and a new genus of ctenocystoid together with the polyplacophoran Solenocaris solenoides and the brachiopod Onniella williamsi. Most of these are multi‐element organisms, with many specimens preserved in an articulated state in finely laminated rocks, indicating minimal disturbance and suggesting that the fauna is largely an in situ association. It contains few of the species known from other deep‐water sites of similar age at Girvan which contain diverse assemblages of trilobites and brachiopods absent from the Lagerstätte. The taphonomy of the site indicates preservation by rapid burial followed by early diagenesis under dysaerobic conditions. It provides a ‘taphonomic window’ on otherwise unknown faunas from distal shelf facies on the Ordovician Laurentian margin, and, moreover, is an important reminder of the hidden biodiversity that resided in thin‐shelled, multi‐element organisms. 相似文献
37.
DENIS F. OWEN F.L.S. DAVID A. S. SMITH F.L.S. 《Biological journal of the Linnean Society. Linnean Society of London》1990,39(3):251-267
Samples of the meadow brown butterfly, Maniola jurtina , were collected from all five of the Canary Islands where it occurs. The butterflies were scored for several spotting characters on both fore- and hindwings and assessed for damage from predators.
The analysis reveals considerable interisland heterogeneity for spotting characters and suggests that the populations fall into two relatively distinct, homogeneous groups, which inhabit the islands of Gran Canaria and La Gomera, on the one hand, and Tenerife, El Hierro and La Palma, on the other.
The main predators are lizards and insectivorous birds. Predator damage is non-random in the following respects: males are more damaged than females; high spot male phenotypes are more damaged than low ones early in the flight period (April-May); symmetrical damage, probably acquired while resting, is commoner in females, while asymmetrical damage is more characteristic of males. Damage generally increases in both sexes through the flight period.
Comparison of the bulked Canary Island samples with museum material from North Africa and Iberia suggests that the former area is the more likely source of the island populations. However, samples from two islands more resemble Iberian stock and it is suggested that the butterfly may have colonized the islands, either by introduction or immigration, on two occasions. 相似文献
The analysis reveals considerable interisland heterogeneity for spotting characters and suggests that the populations fall into two relatively distinct, homogeneous groups, which inhabit the islands of Gran Canaria and La Gomera, on the one hand, and Tenerife, El Hierro and La Palma, on the other.
The main predators are lizards and insectivorous birds. Predator damage is non-random in the following respects: males are more damaged than females; high spot male phenotypes are more damaged than low ones early in the flight period (April-May); symmetrical damage, probably acquired while resting, is commoner in females, while asymmetrical damage is more characteristic of males. Damage generally increases in both sexes through the flight period.
Comparison of the bulked Canary Island samples with museum material from North Africa and Iberia suggests that the former area is the more likely source of the island populations. However, samples from two islands more resemble Iberian stock and it is suggested that the butterfly may have colonized the islands, either by introduction or immigration, on two occasions. 相似文献
38.
The Kosi coastal lake system, a chain of four interconnected basins, is located in the subtropical north-eastern corner of South Africa. Little information is available on zooplankton of the system and the main aim of this study is to report on zooplankton samples collected during 2002 and 2003. The set of samples consists of seasonal, subsurface mesozooplankton samples that were collected during nighttime in each of the lakes. A well-developed salinity gradient was evident along the interconnected lakes in the subsurface water during all seasons, ranging from freshwater in the upper lake Amanzamnyama to a maximum of 22 recorded in Lake Makhawulani. The zooplankton community structures of the lakes reflected the salinity gradient of the system, with some coastal marine taxa recorded in the lakes closer to the mouth and only freshwater taxa recorded in Lake Amanzamnyama. Mesozooplankton diversity and abundance were relatively low compared to other estuarine systems along the eastern coast of South Africa. The dominant taxa were calanoid copepods Acartiella natalensis and Pseudodiaptomus stuhlmanni and the mysid Mesopodopsis africana in the lower lakes, whereas cyclopoids Mesocyclops sp. and Thermocyclops sp. dominated the freshwater lake Amanzamnyama. 相似文献
39.
P. C. OWEN 《The Annals of applied biology》1957,45(3):456-461
The rate of photosynthesis of tobacco leaves infected with the Rothamsted type culture of tobacco mosaic virus was lower than that of comparable healthy tobacco leaves. The lower rate was inferred from Net Assimilation Rates of whole plants and confirmed by direct comparisons of photosynthetic rates of inoculated and healthy leaves. The effect began within 1 hr. of inoculation. It was not caused by an effect of the virus on the stomata, and inactivated virus inoculum did not change the rates. The results indicate either a more rapid movement of virus from the epidermis into the chlorenchyma than has been previously recorded or an effect of virus infection at a site distant from the cells containing virus. 相似文献
40.
HAROLD OWEN 《The Annals of applied biology》1956,44(2):307-321
Quantitative data are provided for the occurrence of Calonectria rigidiuscula and other fungi in occluded Mirid lesions and other sites in shoots of cacao plants. Mirid lesions appear to be particularly favourable for the development of C. rigidiuscula. The fungus also occurs as a saprophyte on cacao pods, and as a wound parasite in woody plants other than cacao; it was successfully introduced into plants of varied affinities by inoculation. C. rigidiuscula spreads from inoculations in cacao stems much more rapidly than other fungi. The results confirm that it is the most important fungus infecting Mirid lesions and causing dieback of cacao in West Africa.
Inoculation tests with a range of Amelonado and Trinitario clones suggested that the clones vary in their susceptibility to C. rigidiuscula , but the plant-to-plant variation was too great to conclude that any one is highly resistant. Various types of introduced cacao were also tested; preliminary experiments indicate that certain types of Upper Amazon cacao may be resistant, but they need further investigation. 相似文献
Inoculation tests with a range of Amelonado and Trinitario clones suggested that the clones vary in their susceptibility to C. rigidiuscula , but the plant-to-plant variation was too great to conclude that any one is highly resistant. Various types of introduced cacao were also tested; preliminary experiments indicate that certain types of Upper Amazon cacao may be resistant, but they need further investigation. 相似文献