全文获取类型
收费全文 | 120182篇 |
免费 | 9992篇 |
国内免费 | 48篇 |
专业分类
130222篇 |
出版年
2021年 | 1086篇 |
2018年 | 1551篇 |
2017年 | 1466篇 |
2016年 | 2010篇 |
2015年 | 2365篇 |
2014年 | 2742篇 |
2013年 | 3919篇 |
2012年 | 4306篇 |
2011年 | 4435篇 |
2010年 | 3326篇 |
2009年 | 2755篇 |
2008年 | 3874篇 |
2007年 | 3865篇 |
2006年 | 3648篇 |
2005年 | 3473篇 |
2004年 | 3578篇 |
2003年 | 3400篇 |
2002年 | 3171篇 |
2001年 | 5063篇 |
2000年 | 4808篇 |
1999年 | 3851篇 |
1998年 | 1373篇 |
1997年 | 1373篇 |
1996年 | 1252篇 |
1995年 | 1131篇 |
1994年 | 1067篇 |
1993年 | 1101篇 |
1992年 | 3015篇 |
1991年 | 2929篇 |
1990年 | 2967篇 |
1989年 | 2709篇 |
1988年 | 2484篇 |
1987年 | 2394篇 |
1986年 | 2193篇 |
1985年 | 2189篇 |
1984年 | 1890篇 |
1983年 | 1603篇 |
1982年 | 1204篇 |
1981年 | 1138篇 |
1979年 | 1756篇 |
1978年 | 1387篇 |
1977年 | 1294篇 |
1976年 | 1183篇 |
1975年 | 1382篇 |
1974年 | 1486篇 |
1973年 | 1539篇 |
1972年 | 1338篇 |
1971年 | 1221篇 |
1970年 | 1037篇 |
1969年 | 1081篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
81.
Targeting of many transmembrane proteins to post-Golgi compartments is dependent on cytoplasmically exposed sorting signals. The most widely used signals conform to the tyrosine- or the leucine-based motifs. Both types of signals have been implicated in protein localization to the same intracellular compartments, but previous results from both cell-free experiments and studies of transfected cell lines have indicated that the two types of signals interact with separate components of the sorting machinery. We have overexpressed several transmembrane proteins in stably transfected Madin-Darby canine kidney cells using an inducible promoter system. Overexpression of proteins containing tyrosine- or leucine-based sorting signals resulted in reduced internalization of the transferrin receptor, whereas recycling and polarized distribution was not influenced. Our results indicate that proteins with tyrosine- and leucine-based sorting signals can be transported along common saturable pathways. 相似文献
82.
83.
84.
85.
Calcium mobilization induced by phosphorylated sphingoid bases was analyzed in calf pulmonary artery endothelial cells by confocal microscopy. A sphingenine-1-phosphate (SeP) analogue, N-acetyl-sphingenine-1-phosphate (N-C2-SeP), exogenously added to these cells, caused a fast and transient intracellular rise in calcium and was as potent as SeP. A minimal concentration of 0.6 nM for N-C2-SeP versus 1 nM for SeP was determined. The N-C2-SeP-induced Ca2+-signaling, like the response to SeP, was due to a release from thapsigargin-sensitive, ryanodine-insensitive, intracellular Ca2+-stores and not to a Ca2+-influx. N-C2-SeP can be considered as a truncated ceramide-phosphate, a lipid already reported to be mitogenic (Gomez-Munoz, A., Duffy, P.A., Martin, A., O'Brien, L., Byun, H.S., Bittman, R. and Brindley, D.N. (1995) Mol. Pharmacol. 47, 833-839), an effect that might be secondary to Ca2+-mobilization. 相似文献
86.
Theo J. Visser Ellen Van Overmeeren-Kaptein 《Biochimica et Biophysica Acta (BBA)/General Subjects》1980,631(2):246-252
A radioimmunoassay for 3′-iodothyronine has been developed. All iodothyronine analogues (except 3,3′-diiodothyronine) showed very little (0.02% at most) cross-reactivity, and the assay was sensitive to 1 pg 3′-iodothyronine/ tube. We have studied the 5′-deiodination of 3′,5′-diiodothyronine by rat liver microsomal fraction in the presence of dithiothreitol. Production of 3′-iodothyronine at 37°C was found to be linear with time of incubation up to 30 min and with concentration of microsomal protein up to 100 μg/ml. The reaction rate reached a limit on increasing 3′,5′-diiodothyronine concentration to 10 μM. The effect of pH on 3′-iodothyronine production was found to depend on 3′,5′-diiodothyronine concentration. Increasing 3′,5′-diiodothyronine concentration from 0.1 to 10 μM resulted in a shift of the pH optimum from 6–6.5 to 7.5. Similar effects on the 5′-deiodination of 3,3′,5′-triiodothyronine were observed, supporting the hypothesis that these reactions are catalysed by a single enzyme (iodothyronine 5′-deiodinase). 相似文献
87.
Development and evaluation of a starter culture for the industrial production of gari 总被引:1,自引:0,他引:1
Gari starter cultures (Gastat) were developed by mixing pure single strains of the organisms that ferment cassava. They were propagated and maintained as granules on dried cocoyam slurry. The cultures were tested for fermentative and acid-producing activity. The acidity produced at 30°C varied from 0.07% to 0.85% lactic acid with maximum levels occurring after 48 h. High levels of reducing sugar were produced during the first 24 h. The amounts produced were about 50% more than those from the self-inoculated cassava. The quality of the gari produced by the starter cultures was good and well accepted. The texture was similar to that produced by natural fermentation. These results highlight the possibility of using starter cultures in the large-scale production of gari. 相似文献
88.
J T Brosnan M E Brosnan M Yudkoff I Nissim Y Daikhin A Lazarow O Horyn I Nissim 《The Journal of biological chemistry》2001,276(34):31876-31882
We have utilized [(15)N]alanine or (15)NH(3) as metabolic tracers in order to identify sources of nitrogen for hepatic ureagenesis in a liver perfusion system. Studies were done in the presence and absence of physiologic concentrations of portal venous ammonia in order to test the hypothesis that, when the NH(4)(+):aspartate ratio is >1, increased hepatic proteolysis provides cytoplasmic aspartate in order to support ureagenesis. When 1 mm [(15)N]alanine was the sole nitrogen source, the amino group was incorporated into both nitrogens of urea and both nitrogens of glutamine. However, when studies were done with 1 mm alanine and 0.3 mm NH(4)Cl, alanine failed to provide aspartate at a rate that would have detoxified all administered ammonia. Under these circumstances, the presence of ammonia at a physiologic concentration stimulated hepatic proteolysis. In perfusions with alanine alone, approximately 400 nmol of nitrogen/min/g liver was needed to satisfy the balance between nitrogen intake and nitrogen output. When the model included alanine and NH(4)Cl, 1000 nmol of nitrogen/min/g liver were formed from an intra-hepatic source, presumably proteolysis. In this manner, the internal pool provided the cytoplasmic aspartate that allowed the liver to dispose of mitochondrial carbamyl phosphate that was rapidly produced from external ammonia. This information may be relevant to those clinical situations (renal failure, cirrhosis, starvation, low protein diet, and malignancy) when portal venous NH(4)(+) greatly exceeds the concentration of aspartate. Under these circumstances, the liver must summon internal pools of protein in order to accommodate the ammonia burden. 相似文献
89.
90.