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排序方式: 共有182条查询结果,搜索用时 78 毫秒
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83.
Greffes de cornée automatisées par laser femtoseconde optimisé et système de contrôle aberrométrique
K. Plamann F. Aptel N. Château C. Crotti F. Deloison F. Druon P. Georges M. Hanna B. Lamory J.-M. Legeais X. Levecq F. Morin V. Nuzzo D.-A. Peyrot M. Savoldelli 《IRBM》2010,31(2):97-100
We present a first evaluation of the results of the GRECO project which aims to conceive and realise an innovative device for ocular surgery by ultrafast pulse laser optimised for corneal grafting. 相似文献
84.
Apoptotic index or a combination of Bax/Bcl-2 expression correlate with survival after resection of pancreatic adenocarcinoma 总被引:1,自引:0,他引:1
Magistrelli P Coppola R Tonini G Vincenzi B Santini D Borzomati D Vecchio F Valeri S Castri F Antinori A Nuzzo G Caraglia M Picciocchi A 《Journal of cellular biochemistry》2006,97(1):98-108
In the present study, the prognostic impact of factors involved in the apoptosis pathway were tested on 67 consecutive patients treated with surgical resection. Included in the study were all patients resected for pancreatic adenocarcinoma from 1988 to 2003. Expression analysis for p53, Bax, and Bcl-2 were performed by immunohistochemical staining. Apoptotic cells were identified by the TUNEL method. These data were correlated with survival. Sixty-seven tumor specimens were included in the study. A strong positive correlation was recorded between p53 overexpression and Bax expression levels (P < 0.001). By univariate analysis, overall survival seemed to be improved with Bcl-2 and Bax expression (respectively, P = 0.0379 and 0.0311). The median survival time in patients with low apoptotic index was better versus those with a high index (P = 0.0127). Lymph node involvement was the only clinico-pathologic parameter that significantly correlated with overall survival (P = 0.0202). By a multivariate Cox regression analysis, the only immunohistochemical parameter that influenced overall survival was the apoptotic index (P = 0.040). Tumor's overexpression of both Bax and Bcl-2 resulted the strongest independent prognostic factor (P = 0.013). This is the first study to report a statistically significant association of apoptosis to overall survival for pancreatic cancer patients treated with surgical resection. The contemporary overexpression of Bax and Bcl-2 represents the strongest prognostic factor. 相似文献
85.
Xander W Huijsdens Beatrix J van Dijke Emile Spalburg Marga G van Santen-Verheuvel Max EOC Heck Gerlinde N Pluister Andreas Voss Wim JB Wannet Albert J de Neeling 《Annals of clinical microbiology and antimicrobials》2006,5(1):1-4
Background
Female genital tuberculosis is an uncommon disease that is rarely diagnosed in developed countries.Case presentation
A 61-year-old postmenopausal woman who had undergone surgery and treated with adjuvant chemotherapy for infiltrating ductal carcinoma of the breast five years ago, presented with bloody vaginal discharge, fatigue, weight loss, and low grade fevers at night for two months. Histological examination of the endometrium, done based on the suspicion of a second primary cancer due to the tamoxifen therapy, revealed a granulomatous reaction. Liquid and solid mycobacterial cultures of the tissues were performed. Although the acid fast staining was negative, the liquid culture was positive for Mycobacterium tuberculosis. Involvement of other systems was not detected. The patient was treated with a three-drug antituberculosis regimen for 9 months and recovered fully.Conclusion
Female genital tuberculosis is a rare but curable disease that should be included in the differential diagnosis of women with menstrual problems. Early diagnosis is important and may prevent unnecessary invasive procedures for the patient. 相似文献86.
Nuzzo JB 《Biosecurity and bioterrorism : biodefense strategy, practice, and science》2006,4(2):147-159
In addition to providing potable drinking water, U.S. water systems are critical to the maintenance of many vital public services, such as fire suppression and power generation. Disruption of these systems would produce severe public health and safety risks, as well as considerable economic losses. Thus, water systems have been designated as critical to national security by the U.S. government. Previous outbreaks of waterborne disease have demonstrated the vulnerability of both the water supply and the public's health to biological contamination of drinking water. Such experiences suggest that a biological attack, or even a credible threat of an attack, on water infrastructure could seriously jeopardize the public's health, its confidence, and the economic vitality of a community. Despite these recognized vulnerabilities, protecting water supplies from a deliberate biological attack has not been sufficiently addressed. Action in this area has suffered from a lack of scientific understanding of the true vulnerability of water supplies to intentional contamination with bioweapons, insufficient tools for detecting biological agents, and a lack of funds to implement security improvements. Much of what is needed to address the vulnerability of the national water supply falls outside the influence of individual utilities. This includes developing a national research agenda to appropriately identify and characterize waterborne threats and making funds available to implement security improvements. 相似文献
87.
Daniele Di Nuzzo Gert‐Jan A. H. Wetzelaer Ricardo K. M. Bouwer Veronique S. Gevaerts Stefan C. J. Meskers Jan C. Hummelen Paul W. M. Blom René A. J. Janssen 《Liver Transplantation》2013,3(1):85-94
The limits of maximizing the open‐circuit voltage Voc in solar cells based on poly[2,7‐(9,9‐didecylfluorene)‐alt‐5,5‐(4,7‐di‐2‐thienyl‐2,1,3‐benzothiadiazole)] (PF10TBT) as a donor using different fullerene derivatives as acceptor are investigated. Bulk heterojunction solar cells with PF10TBT and [6,6]‐phenyl‐C61‐butyric acid methyl ester (PCBM) give a Voc over 1 V and a power conversion efficiency of 4.2%. Devices in which PF10TBT is blended with fullerene bisadduct derivatives give an even higher Voc, but also a strong decrease in short circuit current (Jsc). The higher Voc is attributed to the higher LUMO of the acceptors in comparison to PCBM. By investigating the photophysics of PF10TBT:fullerene blends using near‐IR photo‐ and electroluminescence, time‐resolved photoluminescence, and photoinduced absorption we find that the charge transfer (CT) state is not formed efficiently when using fullerene bisadducts. Hence, engineering acceptor materials with a LUMO level that is as high as possible can increase Voc, but will only provide a higher power conversion efficiency, when the quantum efficiency for charge transfer is preserved. To quantify this, we determine the CT energy (ECT) and optical band gap (Eg), defined as the lowest first singlet state energy ES1 of either the donor or acceptor, for each of the blends and find a clear correlation between the free energy for photoinduced electron transfer and Jsc. We find that Eg ? qVoc > 0.6 eV is a simple, but general criterion for efficient charge generation in donor‐acceptor blends. 相似文献
88.
Alessandro Rolfo Domenica Giuffrida Anna Maria Nuzzo Daniele Pierobon Simona Cardaropoli Ettore Piccoli Mirella Giovarelli Tullia Todros 《PloS one》2013,8(3)
The objective of the present study was to evaluate whether placental mesenchymal stromal cells (PDMSCs) derived from normal and preeclamptic (PE) chorionic villous tissue presented differences in their cytokines expression profiles. Moreover, we investigated the effects of conditioned media from normal and PE-PDMSCs on the expression of pro-inflammatory Macrophage migration Inhibitory Factor (MIF), Vascular Endothelial Growth Factor (VEGF), soluble FMS-like tyrosine kinase-1 (sFlt-1) and free β-human Chorionic Gonadotropin (βhCG) by normal term villous explants. This information will help to understand whether anomalies in PE-PDMSCs could cause or contribute to the anomalies typical of preeclampsia.
Methods
Chorionic villous PDMSCs were isolated from severe preeclamptic (n = 12) and physiological control term (n = 12) placentae. Control and PE-PDMSCs’s cytokines expression profiles were determined by Cytokine Array. Control and PE-PDMSCs were plated for 72 h and conditioned media (CM) was collected. Physiological villous explants (n = 48) were treated with control or PE-PDMSCs CM for 72 h and processed for mRNA and protein isolation. MIF, VEGF and sFlt-1 mRNA and protein expression were analyzed by Real Time PCR and Western Blot respectively. Free βhCG was assessed by immunofluorescent.Results
Cytokine array showed increased release of pro-inflammatory cytokines by PE relative to control PDMSCs. Physiological explants treated with PE-PDMSCs CM showed significantly increased MIF and sFlt-1 expression relative to untreated and control PDMSCs CM explants. Interestingly, both control and PE-PDMSCs media induced VEGF mRNA increase while only normal PDMSCs media promoted VEGF protein accumulation. PE-PDMSCs CM explants released significantly increased amounts of free βhCG relative to normal PDMSCs CM ones.Conclusions
Herein, we reported elevated production of pro-inflammatory cytokines by PE-PDMSCs. Importantly, PE PDMSCs induced a PE-like phenotype in physiological villous explants. Our data clearly depict chorionic mesenchymal stromal cells as central players in placental physiopathology, thus opening to new intriguing perspectives for the treatment of human placental-related disorders as preeclampsia. 相似文献89.
Gorchov David L. Blossey Bernd Averill Kristine M. Dávalos Andrea Heberling J. Mason Jenkins Michael A. Kalisz Susan McShea William J. Morrison Janet A. Nuzzo Victoria Webster Christopher R. Waller Donald M. 《Biological invasions》2021,23(9):2711-2727
Biological Invasions - Forests in eastern North America are experiencing high densities of white-tailed deer (Odocoileus virginianus) and encroachment by invasive plants, both of which threaten... 相似文献
90.
Clara Natoli Davide Brocco Isabella Sperduti Antonio Nuzzo Nicola Tinari Michele De Tursi Antonino Grassadonia Lorenzo Mazzilli Stefano Iacobelli Teresa Gamucci Patrizia Vici the “FOLLOW-UP” Study Group 《PloS one》2014,9(4)