首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   93篇
  免费   4篇
  2020年   1篇
  2018年   1篇
  2015年   3篇
  2014年   3篇
  2013年   6篇
  2012年   8篇
  2011年   4篇
  2010年   5篇
  2009年   6篇
  2008年   6篇
  2007年   9篇
  2006年   3篇
  2005年   2篇
  2004年   4篇
  2003年   3篇
  2001年   4篇
  1998年   2篇
  1997年   1篇
  1996年   2篇
  1995年   2篇
  1993年   1篇
  1992年   1篇
  1991年   1篇
  1990年   1篇
  1989年   1篇
  1987年   1篇
  1986年   2篇
  1985年   1篇
  1983年   1篇
  1982年   2篇
  1980年   2篇
  1979年   1篇
  1978年   1篇
  1977年   2篇
  1976年   1篇
  1975年   1篇
  1970年   1篇
  1968年   1篇
排序方式: 共有97条查询结果,搜索用时 15 毫秒
31.
This study examined benthic and pelagic rate processes from the perspective of benthic dissolved organic matter (DOM) and its possible role in Aureococcus anophagefferens population dynamics. Sampling was conducted in Quantuck Bay, Long Island, New York, at three times in the summer of 2000 and two times in the summer of 2001. A. anophagefferens exhibited a large bloom between the May and July 2000 sample periods, but a smaller bloom was captured in the September 2000 sampling. Densities throughout 2001 were significantly lower than during 2000. There were few differences in most parameters measured between years, but the largest difference was the seasonal increase in both particulate (POM) and dissolved organic matter (DOM) during 2000 that was not observed during 2001. In particular, DOP accumulated the most, followed by DON and DOC, which resulted in significant seasonal decreases in the C:N:P ratios of the DOM pools. On the contrary, changes in elemental ratios of POM were not observed. The seasonal accumulation of DON appeared to be driven largely (50%) by the flux of DON from the benthos in 2000, but during 2001, all measured DON fluxes were into the sediment from the water column. This is consistent with the lack of accumulation during this year. There was little evidence for changes in microzooplankton grazing pressure between 2000 and 2001, and therefore the accumulation of DON and DOP during 2000 could have provided a competitive advantage to A. anophagefferens over other picoalgal species (e.g., Synechococcus) resulting in the significant blooms observed in 2000.  相似文献   
32.
33.
34.
The term “molecular karyotyping” refers to the genome-wide analysis of copy number variations using arrays that cover the genome with genomic markers with varying density. Currently the main application is the investigation of patients with otherwise unexplained mental retardation and multiple congenital anomalies. Studies of such patients who remained without etiological diagnosis after conventional karyotyping, subtelomeric screening, and targeted molecular–cytogenetic studies for well-known microdeletion syndromes revealed chromosomal microaberrations in about 10% of cases and allowed the delineation of several new microdeletion and microduplication syndromes. Nevertheless, because of the large number of copy number polymorphisms, interpretation of unique findings needs thorough consideration.  相似文献   
35.
Work on the development of noninvasive prenatal tests to avoid risk to the fetus in traditional amniocentesis or chorion villus biopsy has been ongoing for many years. Until recently, most approaches were extremely expensive and limited only to selected applications, thus they failed to develop beyond a “proof-of-principle” status. This has changed radically as a result of the introduction of new sequencing methods, since initial studies have shown that fetal aneuploidies from maternal plasma DNA can be identified correctly. In addition, these techniques make it possible to establish even the mutation status of the fetus. While on the one hand this offers completely new options in prenatal diagnosis, progress of this kind is associated with significant ethical challenges on the other. This overview article presents the development of these new methods.  相似文献   
36.
With a life-time prevalence of about 0.5–1.5%, bipolar (manic depressive) disorder represents a common psychiatric disease. Family, twin, and adoption studies have consistently shown that genetic factors contribute to disease development. Genome-wide linkage studies have detected chromosomal regions that are very likely to harbor predisposing genes. Meta-analyses suggest, however, that the genetic contribution of the individual loci must be relatively small which could be one reason for the difficulties in identifying the genes responsible. Very recently, genome-wide association analyses, investigating hundreds of thousands of single nucleotide polymorphisms (SNPs) in phenotypically well-characterized patient and control cohorts, promise a major breakthrough in search of disease-associated genes.  相似文献   
37.
Haemophilia A and B are caused by various mutations in the factor VIII (FVIII) and factor IX (FIX) genes, respectively. The clinical course of the disease is variable, dependent on the severity of the molecular defect. Nowadays, haemophilia patients can excellently be treated by plasma-derived or recombinant clotting factor concentrates. Thus, bleeding and its consequences can be almost completely prevented with nearly normal quality of life and life expectancy. The most severe complication of this treatment is the formation of antibodies (inhibitors) against the substituted clotting factor. The risk of inhibitor formation correlates significantly with specific mutation types that preclude endogenous factor VIII/IX protein synthesis and can be as high as 20–50%. The information on the expected clinical course is at present the most important indication for FVIII/IX gene analysis. Knowledge of the underlying FVIII/IX gene mutation further allows a reliable and fast carrier diagnosis in female relatives of patients with haemophilia.  相似文献   
38.

Background

The large-scale production of G-protein coupled receptors (GPCRs) for functional and structural studies remains a challenge. Recent successes have been made in the expression of a range of GPCRs using Pichia pastoris as an expression host. P. pastoris has a number of advantages over other expression systems including ability to post-translationally modify expressed proteins, relative low cost for production and ability to grow to very high cell densities. Several previous studies have described the expression of GPCRs in P. pastoris using shaker flasks, which allow culturing of small volumes (500 ml) with moderate cell densities (OD600 ~15). The use of bioreactors, which allow straightforward culturing of large volumes, together with optimal control of growth parameters including pH and dissolved oxygen to maximise cell densities and expression of the target receptors, are an attractive alternative. The aim of this study was to compare the levels of expression of the human Adenosine 2A receptor (A2AR) in P. pastoris under control of a methanol-inducible promoter in both flask and bioreactor cultures.

Results

Bioreactor cultures yielded an approximately five times increase in cell density (OD600 ~75) compared to flask cultures prior to induction and a doubling in functional expression level per mg of membrane protein, representing a significant optimisation. Furthermore, analysis of a C-terminally truncated A2AR, terminating at residue V334 yielded the highest levels (200 pmol/mg) so far reported for expression of this receptor in P. pastoris. This truncated form of the receptor was also revealed to be resistant to C-terminal degradation in contrast to the WT A2AR, and therefore more suitable for further functional and structural studies.

Conclusion

Large-scale expression of the A2AR in P. pastoris bioreactor cultures results in significant increases in functional expression compared to traditional flask cultures.  相似文献   
39.
Brown tide, a bloom of the picoplankter Aureococcus anophagefferens, first appeared in eastern Long Island (Suffolk County) waters in the late spring of 1985, at about the same time it emerged, although to a lesser degree, in Narraganset Bay, RI. Since then, it has recurred sporadically in Suffolk County, and blooms have been reported in New Jersey, Delaware, Maryland, and only one other area of the world, Saldanha Bay, South Africa. Bloom initiation and maintenance within Suffolk County appear to be related to A. anophagefferens’ ability to use dissolved organic nitrogen (DON) during periods of limited dissolved inorganic nitrogen (DIN) availability. Factors controlling DIN availability include groundwater influx related to meteorological conditions, introduction of septic leachate from on-site wastewater treatment systems, and biological removal. The complexity of bloom dynamics is illustrated by a cascade of events in Great South Bay involving shellfish clearing rates, a macroalgal bloom, and microbial decomposition.  相似文献   
40.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号