全文获取类型
收费全文 | 1432篇 |
免费 | 176篇 |
国内免费 | 1篇 |
出版年
2022年 | 10篇 |
2021年 | 44篇 |
2020年 | 6篇 |
2019年 | 13篇 |
2018年 | 22篇 |
2017年 | 21篇 |
2016年 | 27篇 |
2015年 | 48篇 |
2014年 | 59篇 |
2013年 | 64篇 |
2012年 | 88篇 |
2011年 | 79篇 |
2010年 | 52篇 |
2009年 | 45篇 |
2008年 | 76篇 |
2007年 | 87篇 |
2006年 | 88篇 |
2005年 | 67篇 |
2004年 | 83篇 |
2003年 | 67篇 |
2002年 | 55篇 |
2001年 | 64篇 |
2000年 | 55篇 |
1999年 | 43篇 |
1998年 | 19篇 |
1997年 | 12篇 |
1996年 | 14篇 |
1995年 | 20篇 |
1994年 | 20篇 |
1993年 | 11篇 |
1992年 | 33篇 |
1991年 | 23篇 |
1990年 | 26篇 |
1989年 | 20篇 |
1988年 | 14篇 |
1987年 | 13篇 |
1986年 | 8篇 |
1985年 | 10篇 |
1984年 | 12篇 |
1983年 | 4篇 |
1982年 | 9篇 |
1981年 | 6篇 |
1980年 | 5篇 |
1979年 | 11篇 |
1978年 | 9篇 |
1977年 | 13篇 |
1975年 | 4篇 |
1974年 | 4篇 |
1971年 | 5篇 |
1966年 | 3篇 |
排序方式: 共有1609条查询结果,搜索用时 250 毫秒
901.
902.
Kazuhisa Yamasaki Ken Tabuchi Akihiko Takahashi Takeshi Osawa Akira Yoshioka Yasushi Ishigooka Shigeto Sudo Mayura B. Takada 《Ecology and evolution》2021,11(23):16936
The mirid bugs Stenotus rubrovittatus and Trigonotylus caelestialium, which cause pecky rice, have become a threat to rice cultivation in Asia. Damage caused by these pests has rapidly become frequent since around 2000 in Japan. Their expansion pattern is not simple, and predicting their future spread remains challenging. Some insects with wide ranges have locally adapted variations in life‐history traits. We performed laboratory rearing experiments to assess the geographical scale of intraspecific variations in life‐history traits of S. rubrovittatus and T. caelestialium. The experiments were aimed at increasing the accuracy of occurrence estimates and the number of generations per year. These results were compared with previous research, and differences in development rates were observed between populations of different latitudes, but not of the same latitude. Finally, plotting the timing of adult emergence and the potential number of generations per year on maps with a 5‐km grid revealed that they differed greatly locally at the same latitude. These maps can be used for developing more efficient methods of managing mirid bugs in integrated pest management. 相似文献
903.
High mobility group-like protein in bovine milk stimulates the proliferation of osteoblastic MC3T3-E1 cells. 总被引:2,自引:0,他引:2
J Yamamura Y Takada M Goto M Kumegawa S Aoe 《Biochemical and biophysical research communications》1999,261(1):113-117
The active component in bovine milk on the proliferation of osteoblastic MC3T3-E1 cells was purified and identified. Growth-promoting activity was measured by [(3)H]thymidine incorporation on the cell. The molecular weight of the purified protein was 10 kDa. The amino-terminal sequence of this 10-kDa protein was identical to bovine high mobility group protein (HMG) 1. This 10-kDa protein is suggested to be a basic protein and to have an HMG box, a consensus sequence motif among the HMG family. From these results, we named this protein HMG-like protein. HMG is a ubiquitous nonhistone component of chromatin and considered to be implicated in DNA replication. We found this protein in milk, and it showed a growth-promoting activity. We propose the possibility that HMG-like protein existed in milk and plays an important role for neonate in bone formation by activating osteoblasts. 相似文献
904.
905.
Tanaka Yoshiko Kawamoto Keizo Kawai Kenichi Takada Jitsuya Fujii Noriko Akaboshi Mitsuhiko 《Biological trace element research》1999,(1):359-363
HeLa S-3 cells were grown in minimal essential medium supplemented with 10% calf serum and 1 mM L-glutamine without adding any rare earth elements (REEs). Exponentially growing cells were collected, and dried materials
were used to analyze their REE content by inductively coupled plasma-mass spectrometry. The results showed that the cells
accumulated REEs in individually different manners; namely the accumulation ratio was higher in the lighter REEs than in the
heavier REEs. To deduce the implication of the accumulation of REEs in HeLa cells, the accumulation ratios for REEs were compared
with those of other biologically important elements. It was seen that the accumulation ratios obtained for REEs (from 31.8
[Ce] to 14.7 [Lu]) were intermediate among those of many bioelements: Fe (124), Mg (54.5), K (38.8), Cr (12.7), Na (11.8),
Mn (11.3), Zn (10.7), Ca (8.8), and V (6.7). 相似文献
906.
Akaboshi Mitsuhiko Kawai Kenichi Tanaka Yoshiko Takada Jitsuya Fujii Noriko 《Biological trace element research》1999,(1):585-593
HeLa S-3 cells were treated with195mPt-radiolabeledcisdiamine(glylato)platinum(II) (254-S) for 60 min at various temperatures, and the relationship between the lethal effect and the number
of Pt atoms binding to DNA, RNA, and proteins was examined. The mean lethal concentration (Do) of 254-S for a 60-min treatment
at 0‡C, 25‡C, 37‡C, 40‡C, 42‡C, and 44‡C was 233,132, 61.1, 42.7, 25.6, and 9.9 ΜM, respectively. By using identically treated
cells, the numbers of Pt atoms combined with DNA, RNA, and protein molecules were determined in the subcellular fractions.
Thus, the D0 values given as drug concentrations were replaced with the number of Pt atoms combined in each fraction. Then, the cell-killing
efficiency of the Pt atom was expressed as the reciprocal of the number of Pt atoms combined and was calculated for each molecule.
The efficiency for the DNA molecule was 0.61X104, 1.09xl04, 1.88xl04, 1.90xl04, 2.66xl04, and 5.88xl04 nucleotides, respectively, for the conditions described. From 0‡C to 44‡C, the cell-killing efficiency of Pt atoms increased
by a factor of 9.6. 相似文献
907.
Epstein-Barr Virus Promotes Epithelial Cell Growth in the Absence of EBNA2 and LMP1 Expression 总被引:5,自引:3,他引:2
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Jun Nishikawa Shosuke Imai Takanori Oda Toshichika Kojima Kiwamu Okita Kenzo Takada 《Journal of virology》1999,73(2):1286-1292
We attempted to infect primary gastric epithelia (PGE) with recombinant Epstein-Barr virus (EBV) carrying a selectable marker that made it possible to select EBV-infected cells. Cells dually positive for EBV-determined nuclear antigen (EBNA) and cytokeratin were detected in 3 of 21 primary cultures after 3 days of EBV inoculation. From one culture, EBV-infected cell clones were repeatedly obtained at a frequency of 3 to 5 cell clones per 106 cells. EBV-infected clones had enhanced population doubling and grew to attain a highly increased saturation density, together with acquisition of marked anchorage independence. The infected clones retained the ultrastructural morphology characteristic of gastric mucosal epithelium and have been growing stably for more than 18 months (corresponding to at least 300 generations) so far, in clear contrast to the parental PGE cells, which ceased growth after 60 generations. The p53 gene of the parental PGE cells was found to be overexpressed, perhaps thereby conferring the basal potential for long-term survival in vitro. Moreover, EBV infection accelerated, to a significant extent, the growth rate and agar clonability of NU-GC-3 cells, an established EBV-negative but EBV-susceptible human gastric carcinoma cell line. Both EBV-converted PGE and NU-GC-3 clones, like EBV-positive gastric carcinoma biopsy specimens, expressed a restricted set of EBV latent infection genes characterized by the absence of EBNA2 and latent membrane protein 1 (LMP1) expression. These results indicate that EBV infection causes a transformed phenotype on PGE in the setting of possible unregulated cell cycling and renders even established gastric carcinoma cells more malignant via a limited spectrum of viral latent-gene expression. This study may reflect an in vivo scenario illustrating multiphasic involvement of EBV in carcinogenesis of gastric or other epithelial cancers. 相似文献
908.
The anamnestic neutralizing antibody response is critical for protection of mice from challenge following vaccination with a plasmid encoding the Japanese encephalitis virus premembrane and envelope genes.
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
E Konishi M Yamaoka I Khin-Sane-WinKurane K Takada P W Mason 《Journal of virology》1999,73(7):5527-5534
For Japanese encephalitis (JE), we previously reported that recombinant vaccine-induced protection from disease does not prevent challenge virus replication in mice. Moreover, DNA vaccines for JE can provide protection from high challenge doses in the absence of detectable prechallenge neutralizing antibodies. In the present study, we evaluated the role of postchallenge immune responses in determining the outcome of JE virus infection, using mice immunized with a plasmid, pcDNA3JEME, encoding the JE virus premembrane (prM) and envelope (E) coding regions. In the first experiment, 10 mice were vaccinated once (five animals) or twice (remainder) with 100 micrograms of pcDNA3JEME. All of these mice showed low (6 of 10) or undetectable (4 of 10) levels of neutralizing antibodies. Interestingly, eight of these animals showed a rapid rise in neutralizing antibody following challenge with 10,000 50% lethal doses of JE virus and survived for 21 days, whereas only one of the two remaining animals survived. No unimmunized animals exhibited a rise of neutralizing antibody or survived challenge. Levels of JE virus-specific immunoglobulin M class antibodies were elevated following challenge in half of the unimmunized mice and in the single pcDNA3JEME-immunized mouse that died. In the second experiment, JE virus-specific primary cytotoxic T-lymphocyte (CTL) activity was detected in BALB/c mice immunized once with 100 micrograms of pcDNA3JEME 4 days after challenge, indicating a strong postchallenge recall of CTLs. In the third experiment, evaluation of induction of CTLs and antibody activity by plasmids containing portions of the prM/E cassette demonstrated that induction of CTL responses alone were not sufficient to prevent death. Finally, we showed that antibody obtained from pcDNA3JEME-immunized mice 4 days following challenge could partially protect recipient mice from lethal challenge. Taken together, these results indicate that neutralizing antibody produced following challenge provides the critical protective component in pcDNA3JEME-vaccinated mice. 相似文献
909.
PI 3-kinase gamma and protein kinase C-zeta mediate RAS-independent activation of MAP kinase by a Gi protein-coupled receptor. 总被引:9,自引:0,他引:9
下载免费PDF全文
![点击此处可从《The EMBO journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
H Takeda T Matozaki T Takada T Noguchi T Yamao M Tsuda F Ochi K Fukunaga K Inagaki M Kasuga 《The EMBO journal》1999,18(2):386-395
Receptors coupled to the inhibitory G protein Gi, such as that for lysophosphatidic acid (LPA), have been shown to activate MAP kinase through a RAS-dependent pathway. However, LPA (but not insulin) has now been shown to activate MAP kinase in a RAS-independent manner in CHO cells that overexpress a dominant-negative mutant of the guanine nucleotide exchange protein SOS (CHO-DeltaSOS cells). LPA also induced the activation of MAP kinase kinase (MEK), but not that of RAF1, in CHO-DeltaSOS cells. The RAS-independent activation of MAP kinase by LPA was blocked by inhibitors of phosphatidylinositol 3-kinase (PI3K) or by overexpression of a dominant-negative mutant of the gamma isoform of PI3K. Furthermore, LPA induced the activation of the atypical zeta isoform of protein kinase C (PKC-zeta) in CHO-DeltaSOS cells in a manner that was sensitive to wortmannin or to the dominant-negative mutant of PI3Kgamma, and overexpression of a dominant-negative mutant of PKC-zeta inhibited LPA-induced activation of MAP kinase. These observations indicate that Gi protein-coupled receptors induce activation of MEK and MAP kinase through a RAS-independent pathway that involves PI3Kgamma-dependent activation of atypical PKC-zeta. 相似文献
910.
Structural requirements of lipid A species in activation of clotting enzymes from the horseshoe crab, and the human complement cascade 总被引:9,自引:0,他引:9
H Takada S Kotani S Tanaka T Ogawa I Takahashi M Tsujimoto T Komuro T Shiba S Kusumoto N Kusunose 《European journal of biochemistry》1988,175(3):573-580
The structure/activity relationship of lipid A, a bioactive center of endotoxic lipopolysaccharides, in the activation of the clotting enzyme cascade of a horseshoe crab amoebocyte lysate (Limulus activity) and the complement system in human serum, was examined using synthetic lipids A and related compounds. Regarding Limulus activity, a newly developed colorimetric method, which utilizes a mixture of recombined clotting factors and a chromogenic substance, was much more sensitive for detecting changes in the chemical structure of test compounds than the conventional gelation method using the amoebocyte whole lysate. (beta 1-6)-D-Glucosamine disaccharide bisphosphates, which had neither 3-hydroxyacyl nor 3-acyloxyacyl groups, and acylglucosamine phosphates, which in structure correspond or are analogous to the non-reducing or reducing moieties of lipids A and biosynthetic disaccharide lipid A precursors showed only negligible activity in the colorimetric tests, but they exhibited a distinct though much weaker gelation activity than the parent disaccharide molecules. The assay results obtained by the colorimetric Limulus test correlate better with the pyrogenicity of the test synthetic compounds than those given by the gelation method, although the dependence of pyrogenicity on chemical structure is greater. The presence of 3-hydroxyacyl groups on the bisphosphorylated (beta 1-6)-D-glucosamine disaccharide backbone is the prerequisite for effective activation of the clotting enzyme cascade of horseshoe crab amoebocyte lysate, while the presence of an adequate number (one or two) of 3-acyloxyacyl groups on the disaccharide bisphosphate backbone is needed for full pyrogenicity. Complement activation, on the other hand, showed structural requirements quite different from those for the colorimetric Limulus activity and the pyrogenicity. The disaccharide compounds that had only non-hydroxylated acyl groups, acylated glucosamine phosphates that had the structure of the non-reducing portion of lipids A and biosynthetic disaccharide precursors, which were scarcely active in the colorimetric Limulus test, caused complement activation comparable to or sometimes stronger than that of the parent disaccharide molecules. Acylglucosamine phosphates, corresponding in structure to the reducing moiety of disaccharide compounds, however, showed little activity. 相似文献