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Two copies of the 16S rRNA gene, rrnA and rrnB, of the type strain 17T of the thermophilic sulfate-reducing bacterium Desulfotomaculum kuznetsovii were cloned and completely sequenced. The comparison of the determined sequences revealed considerable heterogeneity (8.3%) of the two genes, rrnA and rrnB. The main differences were associated with superlong inserts located at the variable 5'- and 3'-terminal regions of the 16S rRNA genes. Comparative analysis that involved analogous genes from the phylogenetically closest representatives of the genus Desulfotomaculum showed that disregard of the heterogeneity of the two gene copies distorts the position of the bacterium studied in the phylogenetic tree. 相似文献
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K.?N.?Kozlov L.?Yu.?Novikova I.?V.?Seferova M.?G.?SamsonovaEmail author 《Biophysics》2018,63(1):136-137
New nonlinear regression models were developed to predict climatic factor dependences of the periods of time from sowing to seedling emergence and from seedling emergence to flowering in soybean. The developed regression models are more accurate than earlier models, but are more complex in structure. 相似文献
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Tochilina AG Novikova NA Sokolova KIa Solov'eva IV Belova IV Ivanova TP Lukovnikova LB Golotsyna LN 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2008,(3):69-73
AIM: The aim of the work is the development of laboratory test for indication and identification of Lactobacillus spp. by the polymerase chain reaction. MATERIALS AND METHODS: The work is developed on the base of the GenBank/EMBL data about genetic sequences of the Lactobacillus spp. The sequences of DNA were studied with the help of the ClustalW program. The strains of the Lactobacillus spp., which are the object of the research, have been registered in Russian collection of industrial microorganisms. RESULTS: The laboratory test of nested-PCR for indication and identification L. plantarum, L. fermentum, L. acidophilus, L. delbrueckii, L. casei, L. rhamnosus was performed. The specificity of the nested-PCR was correlated with the control analyses of monoculture Lactobacillus spp. and commercial products. CONCLUSION: The new developed laboratory nested-PCR test may be use in control system of milk foods enriched by probiotic microorganisms. 相似文献