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21.
Novikova Tatyana I. Asbaganov Sergey V. Ambros Elena V. Zaytseva Yulianna G. 《In vitro cellular & developmental biology. Plant》2020,56(3):307-317
In Vitro Cellular & Developmental Biology - Plant - In order to induce in vitro axillary shoot proliferation from single-node explants of Rhododendron mucronulatum Turcz., two techniques of... 相似文献
22.
Dudina L. G. Novikova O. D. Portnyagina O. Yu. Khomenko V. A. Konyshev I. V. Byvalov A. A. 《Applied Biochemistry and Microbiology》2021,57(4):426-433
Applied Biochemistry and Microbiology - The role of the outer membrane components of Yersinia pseudotuberculosis and Yersinia pestis in the adsorption of pseudotuberculous diagnostic bacteriophage... 相似文献
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N. V. Obroucheva I. A. Sin’kevich S. V. Lityagina G. V. Novikova 《Russian Journal of Plant Physiology》2013,60(3):437-441
The validity of the acid-growth hypothesis is proved for the case of cell elongation initiation in germinating seeds of horse chestnut (Aesculus hippocastanum L.), the embryo axes of which are known to extend during the first stages of germination only by cell elongation. During seed imbibition, H+-ion excretion was firstly low; it increased several times prior to radicle emergence and was maintained at a high level during growth initiation and further cell elongation. Cell wall acidification and radicle emergence were enhanced in the presence of 0.02 mM fusicoccin, thus indicating the involvement of the plasma membrane H+-ATPase in the execution of acid growth. The presence of this enzyme and its activator (14-3-3 protein) in microsomal fractions obtained from radicles and hypocotyls of the embryo axes during and after initiation of cell elongation was demonstrated immunochemically. It is supposed that the initiation of cell elongation at early germination occurs via the activation of the plasma membrane H+-ATPase and results in the acidification of cell walls, leading to their higher extensibility, in accordance with the hypothesis of acid growth. 相似文献
25.
Taratuhin O. D. Novikova L. Yu. Seferova I. V. Gerasimova T. V. Nuzhdin S. V. Samsonova M. G. Kozlov K. N. 《Biophysics》2020,65(1):106-117
Biophysics - Abstract—Soybean phenology is strongly influenced by temperature and day length, and phenological records clearly reflect the changes in climatic conditions. A model including... 相似文献
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L. I. Vorob’eva E. Yu. Khodzhaev T. M. Novikova A. L. Mulyukin E. M. Chudinova A. N. Kozlova G. I. El’-Registan 《Microbiology》2013,82(5):594-599
Cross protection of members of the domains Bacteria, Archaea, and lower Eukaryota from stress factors due to the action of extracellular low-molecular metabolites with adaptogenic functions was shown. The adaptogen produced by Luteococcus japonicus subsp. casei and described previously as a reactivating factor (RF) was shown to protect the yeasts Saccharomyces cerevisiae, archaea Haloarcula marismorti, and the cells of higher eukaryotes (HeLa) against weak stressor impacts. Production of an archaeal extracellular metabolite with a weak adaptogenic effect of the producer cells and capable of a threefold increase in survival of heat-inactivated yeast cells was discovered. Our results confirm the similarity of the compensatory adaptive reactions in prokaryotes (bacteria and archaea) and eukaryotes. 相似文献
28.
O. P. Vostrikova M. P. Isaeva G. N. Likhatskaya O. D. Novikova N. Yu. Kim V. A. Khomenko T. F. Solov’eva 《Biochemistry. Biokhimii?a》2013,78(5):496-504
OmpC-like porin was isolated from the outer membrane (OM) of Yersinia enterocolitica cultured at 37°C (the “warm” variant) and its physicochemical and functional properties were studied. The amino acid sequence of OmpC porin was established, and the primary structure and transmembrane topology of this protein were analyzed in comparison with the OmpF porin isolated from Y. enterocolitica cultured at 6°C (the “cold” variant). Both porins of Y. enterocolitica had a high homology degree (65%) between themselves and with OmpC and OmpF porins from OM of Escherichia coli (58 and 76% homology, respectively). The secondary structure of OmpC and OmpF porins from OM of Y. enterocolitica consists of 16 β-strands connected by short “periplasmic” and longer “extracellular” loops with disordered structure, according to the topological model developed for porins of E. coli. The molecular structures of OmpC and OmpF porins of Y. enterocolitica have significant differences in the structure of the “extracellular” loops and in the position of one of three tryptophan residues. Using the bilayer lipid membrane (BLM) technique, pores formed by OmpC porin of Y. enterocolitica were shown to differ in electrophysiological characteristics from channels of OmpF protein of this microorganism. The isolated OmpC porin reconstructed into BLM displayed functional plasticity similarly to OmpF protein and nonspecific porins of other enterobacteria. The conductivity level of the channels formed by this protein in the BLM was regulated by value of the applied potential. 相似文献
29.
A. P. Domnina P. V. Novikova I. I. Fridlyanskaya M. A. Shilina V. V. Zenin N. N. Nikolsky 《Cell and Tissue Biology》2016,10(2):95-99
In this study, we compared the ability of human mesenchymal stem cells (eMSCs) derived from menstrual blood and mesenchymal stem cells (MSCs) from other tissues to differentiate into decidual cells in vitro. It was demonstrated that, during differentiation, secretion of prolactin and insulin-like growth factor binding protein-1 (key decidualization markers) markedly increased in eMSCs slightly augmented in bone marrow MSC (BM-MSCs) and did not change in MSCs from adipose tissue (AT-MSCs). Thus, eMSCs exhibited higher capacity for differentiation into decidual cells than BM-MSCs or AT-MSCs. This makes eMSCs promising for application in cellular therapy of infertility associated with insufficient decidualization of endometrium. 相似文献
30.
T. A. Voeikova A. S. Shebanova Yu. D. Ivanov A. L. Kaysheva L. M. Novikova O. A. Zhuravliova V. V. Shumyantseva K. V. Shaitan M. P. Kirpichnikov V. G. Debabov 《Applied Biochemistry and Microbiology》2016,52(8):769-775
Silver sulfide nanoparticles stable in aqueous solutions were obtained in presence of the cells of the bacterium Shewanella oneidensis MR-1 in aqueous solution containing an equimolar mixture of AgNO3 and Na2S2O3. Proteins absorbed on the surface of Ag2S nanoparticles were identified for the first time by MALDITOF/TOF. Among these proteins, multiheme cytochromes MtrC and OmcA, as well as the MtrB membrane porin, which forms a complex on the outer cell membrane, were detected. It was shown that an insoluble precipitate consisting of agglomerated Ag2S nanoparticles with a wide size distribution was formed in the absence of the cells. The role of the detected proteins in the mechanism of the formation and stabilization of the Ag2S nanoparticles in the studied system is discussed. 相似文献