全文获取类型
收费全文 | 226篇 |
免费 | 17篇 |
出版年
2023年 | 5篇 |
2022年 | 4篇 |
2021年 | 6篇 |
2020年 | 5篇 |
2019年 | 3篇 |
2018年 | 7篇 |
2017年 | 8篇 |
2016年 | 11篇 |
2015年 | 14篇 |
2014年 | 7篇 |
2013年 | 21篇 |
2012年 | 22篇 |
2011年 | 22篇 |
2010年 | 17篇 |
2009年 | 18篇 |
2008年 | 16篇 |
2007年 | 13篇 |
2006年 | 7篇 |
2005年 | 8篇 |
2004年 | 7篇 |
2003年 | 5篇 |
2002年 | 5篇 |
2001年 | 3篇 |
2000年 | 1篇 |
1999年 | 2篇 |
1997年 | 1篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1990年 | 1篇 |
1988年 | 1篇 |
1985年 | 1篇 |
排序方式: 共有243条查询结果,搜索用时 312 毫秒
221.
Tatiana D. Saccon Augusto Schneider Cindi G. Marinho Allancer D. C. Nunes Sarah Noureddine Joseph Dhahbi Yury O. Nunez Lopez Gage LeMunyan Roberto Salvatori Carla R. P. Oliveira Alcia A. OliveiraSantos Nicolas Musi Andrzej Bartke Manuel H. AguiarOliveira Michal M. Masternak 《Aging cell》2021,20(7)
Reduced inflammation, increased insulin sensitivity, and protection against cancer are shared between humans and mice with GH/IGF1 deficiency. Beyond hormone levels, miRNAs are important regulators of metabolic changes associated with healthy aging. We hypothesized that GH deficiency in humans alters the abundance of circulating miRNAs and that a subset of those miRNAs may overlap with those found in GH‐deficient mice. In this study, subjects with untreated congenital isolated GH deficiency (IGHD; n = 23) and control subjects matched by age and sex (n = 23) were recruited and serum was collected for miRNA sequencing. Serum miRNAs from young (6 month) and old (22 month) Ames dwarf (df/df) mice with GH deficiency and their WT littermates (n = 5/age/genotype group) were used for comparison. We observed 14 miRNAs regulated with a genotype by age effect and 19 miRNAs regulated with a genotype effect independent of age in serum of IGHD subjects. These regulated miRNAs are known for targeting pathways associated with longevity such as mTOR, insulin signaling, and FoxO. The aging function was overrepresented in IGHD individuals, mediated by hsa‐miR‐31, hsa‐miR‐146b, hsa‐miR‐30e, hsa‐miR‐100, hsa‐miR‐181b‐2, hsa‐miR‐195, and hsa‐miR‐181b‐1, which target the FoxO and mTOR pathways. Intriguingly, miR‐181b‐5p, miR‐361‐3p, miR‐144‐3p, and miR‐155‐5p were commonly regulated in the serum of humans and GH‐deficient mice. In vitro assays confirmed target genes for the main up‐regulated miRNAs, suggesting miRNAs regulated in IGHD individuals can regulate the expression of age‐related genes. These findings indicate that systemic miRNAs regulated in IGHD individuals target pathways involved in aging in both humans and mice. 相似文献
222.
Mark A. Brooks Kamil Gewartowski Eirini Mitsiki Juliette Létoquart Roland A. Pache Ysaline Billier Michela Bertero Margot Corréa Mariusz Czarnocki-Cieciura Michal Dadlez Véronique Henriot Noureddine Lazar Lila Delbos Dorothée Lebert Jan Piwowarski Pascal Rochaix Bettina Böttcher Luis Serrano Bertrand Séraphin Herman van Tilbeurgh Andrzej Dziembowski 《Structure (London, England : 1993)》2010,18(9):1075-1082
223.
Fawzia Chaabane Chaouch Noureddine Bouras Salim Mokrane Khaoula Bouznada Abdelghani Zitouni Gabriele Pötter Cathrin Spröer Hans-Peter Klenk Nasserdine Sabaou 《Antonie van Leeuwenhoek》2017,110(2):245-252
A filamentous actinobacterium, designated strain PM3T, was isolated from a Saharan soil sample collected from Béni-Abbès, Béchar (South-West Algeria). A polyphasic taxonomic study was carried out to establish the status of strain PM3T. The isolate was found to have morphological and chemotaxonomical properties associated with members of the genus Planomonospora. The new isolated microorganism developed cylindrical sporangia arranged in double parallel rows on aerial mycelium, each one containing a motile single sporangiospore. The cell wall of the strain was found to contain meso-diaminopimelic acid. Whole-cell hydrolysates were found to contain madurose, glucose, mannose and ribose. The predominant menaquinone was identified as MK-9(H2) (69.6%). The polar lipids detected were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, phosphatidylhydroxyethanolamine and glucosamine-containing lipids. The major fatty acids were found to be C17:1ω9c (38.6%) and C17:0 (24.2%). Results of 16S rRNA gene sequence comparison revealed that strain PM3T shared a high degree of 16S rRNA gene sequence similarity with Planomonospora sphaerica DSM 44632T (99.3%), Planomonospora parontospora subsp. parontospora DSM 43177T (99.2%) and P. parontospora subsp. antibiotica DSM 43869T (99.0%). DNA–DNA hybridization values between strain PM3T and the type strains of the closely related species were between 58.4 and 70.1%. The combination of phylogenetic analysis, DNA–DNA relatedness data, phenotypic characteristics and chemotaxonomic data support the conclusion that strain PM3T represents a novel species of the genus Planomonospora, for which the name Planomonospora algeriensis sp. nov. is proposed. The type strain is PM3T (=DSM 46752T = CECT 9047T). 相似文献
224.
Mahfoudh W Bouaouina N Ahmed SB Gabbouj S Shan J Mathew R Uhrhammer N Bignon YJ Troudi W Elgaaied AB Hassen E Chouchane L 《Molecular biology reports》2012,39(2):1037-1046
Germ-line mutations in BRCA1 breast cancer susceptibility gene account for a large proportion of hereditary breast cancer families and show considerable ethnic and geographical variations. The contribution of BRCA1 mutations to hereditary breast cancer has not yet been thoroughly investigated in Middle Eastern and North African populations. In this study, 16 Tunisian high-risk breast cancer families were screened for germline mutations in the entire BRCA1 coding region and exon?Cintron boundaries using direct sequencing. Six families were found to carry BRCA1 mutations with a prevalence of 37.5%. Four different deleterious mutations were detected. Three truncating mutations were previously described: c.798_799delTT (916 delTT), c.3331_3334delCAAG (3450 delCAAG), c.5266dupC (5382 insC) and one splice site mutation which seems to be specific to the Tunisian population: c.212?+?2insG (IVS5?+?2insG). We also identified 15 variants of unknown clinical significance. The c.798_799delTT mutation occurred at an 18% frequency and was shared by three apparently unrelated families. Analyzing five microsatellite markers in and flanking the BRCA1 locus showed a common haplotype associated with this mutation. This suggests that the c.798_799delTT mutation is a Tunisian founder mutation. Our findings indicate that the Tunisian population has a spectrum of prevalent BRCA1 mutations, some of which appear as recurrent and founding mutations. 相似文献
225.
226.
Mohamed El Yaagoubi Hicham Mechqoq Sergio Ortiz Carlos Cavaleiro Marylin Lecsö-Bornet Catarina Guerreiro Pereira Maria João Rodrigues Luísa Custódio Abdelhamid El Mousadik Laurent Picot Marina Kritsanida Fouad Msanda Noureddine El Aouad Raphaël Grougnet 《化学与生物多样性》2021,18(12):e2100653
The chemical composition and in vitro biological activities of the essential oil (EO) of Micromeria macrosiphon Coss. and M. arganietorum (J. Emb.) R. Morales, two Lamiaceae endemic to south Morocco, were investigated. GC/MS analysis resulted in the identification of 36 metabolites from the EO of M. macrosiphon, 45 from M. arganietorum. Borneol was the major metabolite in both oils and together with related derivatives such as camphor, accounted for 2/3 of the EO of M. macrosiphon, 1/3 of those of M. arganietorum. Pinene and terpinene derivatives were also present in high proportions. From a chemotaxonomic point of view, the composition of the examined samples may be related to those of other species endemic to Macaronesia. Both EOs showed significant toxicity towards liver HepG2 and melanoma B16 4A5 tumor cell lines at 100 μg/mL; however, they were also cytotoxic towards S17 normal cell lines, with a selectivity index <1. No antibacterial activity was noticed against 52 strains at 100 μg/mL. 相似文献
227.
228.
229.
M'hamed El Mokhefi Gahdab Chakali Noureddine Rahim Lynda Bouchou Andrea Battisti 《Agricultural and Forest Entomology》2021,23(1):23-31
- Body size correlates with several factors such as reproductive fitness, environmental changes, the quality and quantity of food during critical development stages, and the feeding season. For the Palearctic moths of the genus Thaumetopoea (Lepidoptera; Notodontidae), the larval development is crucial and differs between species according to their feeding season; larvae of the pine processionary moth Thaumetopoea pityocampa (Denis & Schiffermüller 1775) feed during winter while larvae of its congeneric cedar processionary moth Thaumetopoea bonjeani (Powell 1922) develop during summer in North Africa.
- This discrepancy in lifecycles leads to different reproductive traits such as egg batch length, number of eggs per batch, eggs protection mechanisms and female body size.
- According to Darwin's fecundity advantage hypothesis (1871), female body size should have a positive influence on reproductive fitness, since larger females supposedly have higher fecundity. The universal allometric scaling phenomenon rule proposed by Rensch (1950) predicts that the degree of sexual size dimorphism tends to decrease with the increase of female body size.
- Here, two morphometrical parameters that is, body size and scale size, estimated from body measurements of individuals of both species, feeding on the same host Atlantic cedar Cedrus atlantica (Manetti & Carrière 1855) (Pinales; Pinaceae) in Algeria were proposed. The aim was to find out traits that might rule the competition for food and space, in particularly fecundity and body size.
- Results of the present study highlight a female-biased sexual size dimorphism in both species. The positive correlation between female body size and fecundity shown in this study weakly supports Darwin's hypothesis. Finally, the intrageneric test performed leads to conclude that Rensch's rule does not hold in the considered species.