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991.
Summary By use of the indirect immunofluorescent technique applied to whole mounts of tissues and organs of the American cockroach, Periplaneta americana, serotonin-like reactivity has been demonstrated in an extensive meshwork of fibers on the surface of nerves of the subesophageal ganglion and the tritocerebrum. This meshwork appears to serve the neurohemal release of serotonin. In addition, several of these nerves contain two or more centrally located axons that appear to be serotonergic. The corpora cardica show immunoreactivity but do not appear to be a principal release site for serotonin. The nerves and ganglia of the stomadeal nervous system show immunoreactivity for serotonin and provide serotonin-positive innervation to the salivary glands and the visceral muscles of the stomodeum and midgut. The heart, hindgut, Malpighian tubules, fat body, and skeletal muscles all appear to lack serotonin immunoreactivity. 相似文献
992.
Extremely low genetic diversity across mangrove taxa reflects past sea level changes and hints at poor future responses
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Yuchen Yang Wenqing Wang Cairong Zhong Anthony J. Greenberg Chung‐I Wu Norman C. Duke Suhua Shi 《Global Change Biology》2018,24(4):1741-1748
The projected increases in sea levels are expected to affect coastal ecosystems. Tropical communities, anchored by mangrove trees and having experienced frequent past sea level changes, appear to be vibrant at present. However, any optimism about the resilience of these ecosystems is premature because the impact of past climate events may not be reflected in the current abundance. To assess the impact of historical sea level changes, we conducted an extensive genetic diversity survey on the Indo‐Malayan coast, a hotspot with a large global mangrove distribution. A survey of 26 populations in six species reveals extremely low genome‐wide nucleotide diversity and hence very small effective population sizes (Ne) in all populations. Whole‐genome sequencing of three mangrove species further shows the decline in Ne to be strongly associated with the speed of past changes in sea level. We also used a recent series of flooding events in Yalong Bay, southern China, to test the robustness of mangroves to sea level changes in relation to their genetic diversity. The events resulted in the death of half of the mangrove trees in this area. Significantly, less genetically diverse mangrove species suffered much greater destruction. The dieback was accompanied by a drastic reduction in local invertebrate biodiversity. We thus predict that tropical coastal communities will be seriously endangered as the global sea level rises. Well‐planned coastal development near mangrove forests will be essential to avert this crisis. 相似文献
993.
John Fawcett Wilson David Baker Janet Cheney Mike Cook Martin Ellis Roger Freestone David Gardner Graham Geen Robin Hemming Don Hodgers Stephen Howarth Ade Jupp Norman Lowe Steve Orridge Mark Shaw Bob Smith Audrey Turner Heather Young 《Journal of Insect Conservation》2018,22(2):189-196
The abundance of 100 of the most widespread species of macro-moth in the UK and Ireland was estimated from light-trap records reported to the UK and Ireland Garden Moth Scheme between 2005 and 2015. Recording sites were classified into low, medium and high night-time illumination categories by satellite imagery, into land-use types from the dominant use in the 1–2 km surrounding area, and by latitude. Most urban sites were in the group classified as having high night-time illumination. Comparison between medium and low night-time illuminated sites allowed differences between levels of illumination free from the effect of urbanization on moth abundance to be assessed. The medium and low night-time illuminated sites differed significantly in the frequency of grassland and arable land-use types and in geographic location with more grassland and north-western sites in the low-light category. After adjustment for these differences and for variations between taxonomic family groups, the medium to low night-time lighting abundance ratios explained a significant (P?<?0.001) 20% of the variance in long-term changes in moth abundance reported by the Rothamsted Insect Survey for the years 1968–2002. This is the first demonstration that artificial night-time lighting has had, in combination with other factors, a significant influence on levels of abundance of moth populations. 相似文献
994.
Properties of the vastus lateralis muscle in relation to age and physiological function in master cyclists aged 55–79 years
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Ross D. Pollock Katie A. O'Brien Lorna J. Daniels Kathrine B. Nielsen Anthea Rowlerson Niharika A. Duggal Norman R. Lazarus Janet M. Lord Andrew Philp Stephen D. R. Harridge 《Aging cell》2018,17(2)
In this study, results are reported from the analyses of vastus lateralis muscle biopsy samples obtained from a subset (n = 90) of 125 previously phenotyped, highly active male and female cyclists aged 55–79 years in regard to age. We then subsequently attempted to uncover associations between the findings in muscle and in vivo physiological functions. Muscle fibre type and composition (ATPase histochemistry), size (morphometry), capillary density (immunohistochemistry) and mitochondrial protein content (Western blot) in relation to age were determined in the biopsy specimens. Aside from an age‐related change in capillary density in males (r = ?.299; p = .02), no other parameter measured in the muscle samples showed an association with age. However, in males type I fibres and capillarity (p < .05) were significantly associated with training volume, maximal oxygen uptake, oxygen uptake kinetics and ventilatory threshold. In females, the only association observed was between capillarity and training volume (p < .05). In males, both type II fibre proportion and area (p < .05) were associated with peak power during sprint cycling and with maximal rate of torque development during a maximal voluntary isometric contraction. Mitochondrial protein content was not associated with any cardiorespiratory parameter in either males or females (p > .05). We conclude in this highly active cohort, selected to mitigate most of the effects of inactivity, that there is little evidence of age‐related changes in the properties of VL muscle across the age range studied. By contrast, some of these muscle characteristics were correlated with in vivo physiological indices. 相似文献
995.
Pamela H. Scott Nicola J. Veitch Helen Gadegaard Muhammad Mughal Gethin Norman Michelle Welsh 《Journal of biological education》2018,52(2):184-195
Laboratories are recognised as central in science education, allowing students to consolidate knowledge and master practical skills, however, their effectiveness has been questioned. Whilst laboratory practicals are useful for students’ learning of basic procedures, they have been shown to be less effective for developing conceptual understanding of the subject. Interactive lectures and bespoke digital resources were utilised in order to enhance theoretical understanding of laboratory practical molecular sessions, thus enabling students to take responsibility for and direct their own learning, encouraging inquiry-based learning. Providing easy to access additional learning resources offered students an opportunity to better prepare themselves for the laboratory, and consolidate their knowledge through subsequent review and self-testing in their own time. Grades before and after implementation of these active learning strategies were analysed to look at the impact on student learning and this study demonstrates that integrating these into a challenging practical biology course improved grades significantly with a concomitant increase in the number of ‘A’ grades attained. Feedback to evaluate use and perceptions of both interactive lectures and digital resources were also analysed. It has been shown here that these activities enhanced student experience and understanding of the course. 相似文献
996.
Assessment of wound healing of tagged gray (Eschrichtius robustus) and blue (Balaenoptera musculus) whales in the eastern North Pacific using long‐term series of photographs
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Stephanie A. Norman Kiirsten R. Flynn Alexandre N. Zerbini Frances M. D. Gulland Michael J. Moore Stephen Raverty David S. Rotstein Bruce R. Mate Craig Hayslip Diane Gendron Richard Sears Annie B. Douglas John Calambokidis 《Marine Mammal Science》2018,34(1):27-53
Tags have been used to examine migration routes and habitat use of large whales for >40 yr, however, evaluation of tag wound healing has largely been short‐term, anecdotal or generalized. This study developed methods for systematic photographic assessment of long‐term external consequences of tag placement, to determine potential differences in wound healing between species and tag types and thus advise future tagging efforts to possibly minimize undesirable side effects. Tag site appearance and healing characteristics were evaluated by two reviewers and a time series evaluated by five veterinarians from photographs during 995 postdeployment encounters with 34 gray and 63 blue whales tagged in the North Pacific. Blue whale resightings were less frequent, but spanned a longer time period due to earlier tag deployments than the more frequent gray whale follow‐up observations. Swelling occurred in 74% of reencountered gray whales, with the highest frequency 6 mo postdeployment. Swellings were common in blue whales with early tag designs but rare with current models. Depressions occurred in 82% of gray and 71% of blue whales. This study demonstrates the value of follow‐up studies of tagged animals and systematic scoring of photographs to quantitatively compare tag response. 相似文献
997.
Jaya Prabhakaran Mark Underwood Francesca Zanderigo Norman R. Simpson Anna R. Cooper Jeffrey Matthew Harry Rubin-Falcone Ramin V. Parsey J. John Mann J.S. Dileep Kumar 《Bioorganic & medicinal chemistry letters》2018,28(14):2432-2435
Radiosynthesis and in vivo evaluation of [11C]4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl]benzenesulfonamide (methoxy analogue of valdecoxib, [11C]MOV), a COX-2 inhibitor, was conducted in rat and baboon. Synthesis of the reference standard MOV (3), and its desmethyl precursor 2 for radiolabeling were performed using 1,2-diphenylethan-1-one as the starting material in five steps with 15% overall yield. Radiosynthesis of [11C]MOV was accomplished in 40?±?10% yield and?>99% radiochemical purity by reacting the precursor 2 in dimethyl formamide (DMF) with [11C]CH3I followed by removal of the dimethoxytrityl (DMT) protective group using trifluroacetic acid. PET studies in anesthetized baboon showed very low uptake and homogeneous distribution of [11C]MOV in brain. The radioligand underwent rapid metabolism in baboon plasma. MicroPET studies in male Sprague Dawley rats revealed [11C]MOV binding in lower thorax. The tracer binding in rats was partially blocked in heart and duodenum by the administration of 1?mg/kg oral dose of COX-2 inhibitor valdecoxib. 相似文献
998.
Janette A. Norman Les Christidis Richard Schodde 《Organisms Diversity & Evolution》2018,18(2):241-259
Understanding how the complex geotectonic and climatic history of the Australo-Papuan region has promoted the ecological and evolutionary diversification of its avifauna remains a challenge. Outstanding questions relate to the nature and timing of biogeographical connections between Australia and the emerging island of New Guinea and the mechanisms by which distinctive altitudinal replacement sequences have evolved amongst congeneric species in montane New Guinea. Here, we combine analyses of phylogenetic and eco-morphological data to investigate ecological and evolutionary patterns of diversification in the largely mesic-adapted Australo-Papuan scrubwrens (Sericornis) and mouse-warblers (Crateroscelis). We find evidence of ecological convergence and present a revised taxonomic and systematic treatment of the subfamily integrating information from new (ND2) and existing molecular phylogenetic reconstructions. Biogeographical connections indicate at least three phases of faunal interchange between Australia and New Guinea commencing in the mid to late Miocene. We also find little support for the proposed time dependency of ecological sorting mechanisms linked to divergence in foraging niche amongst altitudinal replacements. Instead, physiological adaptations to hypoxia and increased thermal efficiency at higher altitudes may better account for observed patterns of diversification in montane New Guinea. Indirect support for this hypothesis is derived from molecular clock calibrations that indicate a pulse of diversification across the Miocene-Pliocene boundary coincident with a phase of rapid mountain uplift. Simple ecological and climatic models appear inadequate for explaining observed patterns and mechanisms of diversification in the New Guinean montane avifauna. Further insights will require multidisciplinary research integrating geotectonic, palaeoclimatic, genetic, ecological and physiological approaches. 相似文献
999.
John D. Nason Norman C. Ellstrand 《Evolution; international journal of organic evolution》1995,49(2):307-316
Studies of inbreeding depression in plant populations have focused primarily on comparisons of selfing versus outcrossing in self-compatible species. Here we examine the effect of five naturally occurring levels of inbreeding (f ranging from 0 to 0.25 by pedigree) on components of lifetime fitness in a field population of the self-incompatible annual, Raphanus sativus. Pre- and postgermination survival and reproductive success were examined for offspring resulting from compatible cross-pollinations. Multiple linear regression of inbreeding level on rates of fruit and seed abortion as well as seed weight and total seed weight per fruit were not significant. Inbreeding level was not found to affect seed germination, offspring survival in the field, date of first flowering, or plant biomass (dry weight minus fruit). The effect of inbreeding on seedling viability in the greenhouse and viability to flowering was significant but small and inconsistently correlated with inbreeding level. Maternal fecundity, however, a measure of seed yield, was reduced almost 60% in offspring from full-sib crosses (f = 0.25) relative to offspring resulting from experimental outcross pollinations (f = 0). Water availability, a form of physiological stress, affected plant biomass but did not affect maternal fecundity, nor did it interact with inbreeding level to influence these characters. The delayed expression of strong inbreeding depression suggests that highly deleterious recessive alleles were not a primary cause of fitness loss with inbreeding. Highly deleterious recessives may have been purged by bottlenecks in population size associated with the introduction of Raphanus and its recent range expansions. In general, reductions in total relative fitness of greater than 50% associated with full-sib crosses should be sufficient to prohibit the evolution of self-compatibility via transmission advantage in Raphanus. 相似文献
1000.
Angelika Grossmann Peter S. Rabinovitch Mark A. Lane John C. Jinneman Donald K. Ingram Norman S. Wolf Richard G. Cutler George S. Roth 《Journal of cellular physiology》1995,162(2):298-303
The influence of aging and dietary restriction on increase in intracellular free calcium ([Ca2+]i) of CD4+ lymphocytes from Macaca mulatta was examined after stimulation with anti-CD3 mAb. We used a flow cytometric assay with the dye indo-1 and either direct or reciprocal immunofluorescent staining to dientify CD4+ cells. After stimulation with anti-CD3 mAb, intracellular free calcium responses were reduced in CD4+ lymphocytes from old male and female ad libitum fed monkeys compared to young and adult male or female monkeys. Old female monkeys had significantly lower [Ca2+]i than did old male monkeys. The reduced responses were in part related to a decreased percentage of responding cells. Dietary restrition of males over a four-year period did not alter [Ca2+]i response compared to ad libitum fed male monkeys. Female monkeys of all ages (which were restricted only for four months) also had similar [Ca2+]i responses to ad libitum fed controls. Our data suggest that age-related changes in [Ca2+]i responses are similar between humans and M. mulatta, and that over these intervals, no effects of caloric restrictions can be detected. © 1995 Wiley-Liss, Inc. 相似文献