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71.
Shoichi Kubota Yasunori Fukumoto Kenichi Ishibashi Shuhei Soeda Sho Kubota Ryuzaburo Yuki Yuji Nakayama Kazumasa Aoyama Noritaka Yamaguchi Naoto Yamaguchi 《The Journal of biological chemistry》2014,289(9):5730-5746
Mimosine is an effective cell synchronization reagent used for arresting cells in late G1 phase. However, the mechanism underlying mimosine-induced G1 cell cycle arrest remains unclear. Using highly synchronous cell populations, we show here that mimosine blocks S phase entry through ATM activation. HeLa S3 cells are exposed to thymidine for 15 h, released for 9 h by washing out the thymidine, and subsequently treated with 1 mm mimosine for a further 15 h (thymidine → mimosine). In contrast to thymidine-induced S phase arrest, mimosine treatment synchronizes >90% of cells at the G1-S phase boundary by inhibiting the transition of the prereplication complex to the preinitiation complex. Mimosine treatment activates ataxia telangiectasia mutated (ATM)/ataxia telangiectasia and Rad3-related (ATR)-mediated checkpoint signaling without inducing DNA damage. Inhibition of ATM activity is found to induce mimosine-arrested cells to enter S phase. In addition, ATM activation by mimosine treatment is mediated by reactive oxygen species (ROS). These results suggest that, upon mimosine treatment, ATM blocks S phase entry in response to ROS, which prevents replication fork stalling-induced DNA damage. 相似文献
72.
Some critical factors involved in formation of conditioned taste aversion to sodium chloride in rats
Yamamoto Takashi; Shimura Tsuyoshi; Sako Noritaka; Yasoshima Yasunobu; Sakai Nobuyuki 《Chemical senses》1994,19(3):209-217
Some factors concerning acquisition and retention of conditionedtaste aversions (CTAs) were behaviorally examined in the rat.In the CTA paradigm, aqueous solution of 0.1 M NaCl was usedas the conditioned stimulus (CS) and an intraperitoneal (i.p.)injection of 0.15 M LiCl was employed as the unconditioned stimulus(US). In experiment 1, CTAs to 0.1 M NaCl were examined in bothforward (CSUS) and backward (USCS) conditioningparadigms. Reliable CTAs were produced in the USCS conditioningparadigm when the USCS interval was less than 10 min,as well as in the CSUS conditioning paradigm. In experiment2, strong CTAs to 0.1 M NaCl were established when water-deprivedrats made at least 500 continuous licks, corresponding to 2.5ml intake and 2 min of drinking time. In experiment 3, effectsof gustatory deafferentation on CTA formation were studied.Only the chorda tympani played an important role in acquisitionand retention of CTAs to NaCl solutions. These results ipHimMthat strong CTAs can be acquired to 0.1 M NaCl, if its tasteinformation which is conveyed via the chorda tympani duringthe 500 continuous licks is followed by LiCl-induced sickness. 相似文献
73.
Noritaka Nakamura Tsuyoshi Hosoya Chihiro Tanaka Yuko Takeuchi-Kaneko 《Mycoscience》2018,59(5):397-408
We used a culture-dependent approach to investigate root-associated fungal communities in Fagaceae roots at four fagaceous species-dominant forests in Japan. In total 1029 isolates were collected and classified, based on colony morphological features and molecular information. Species of order Helotiales (Ascomycota) were dominantly isolated at all four sites, in which a globally-distributed putative endophytic group in Hyaloscyphaceae predominated. This group of fungi was morphologically and phylogenetically investigated using these isolates as well as additional isolates collected from 8 different sites in Japan. Among the Hyaloscyphaceae, Glutinomyces species were frequently detected, and three novel species, G. inflatus, G. vulgaris, and G. takaragaikensis spp. nov. were identified and described according to their morphology and genealogical concordance. 相似文献
74.
75.
Yuri Miura Noritaka Hashii Yuki Ohta Yoko Itakura Hiroki Tsumoto Junya Suzuki Daisuke Takakura Yukiko Abe Yasumichi Arai Masashi Toyoda Nana Kawasaki Nobuyoshi Hirose Tamao Endo 《Biochimica et Biophysica Acta (BBA)/General Subjects》2018,1862(6):1462-1471
Background
Glycosylation is highly susceptible to changes of the physiological conditions, and accordingly, is a potential biomarker associated with several diseases and/or longevity. Semi-supercentenarians (SSCs; older than 105?years) are thought to be a model of human longevity. Thus, we performed glycoproteomics using plasma samples of SSCs, and identified proteins and conjugated N-glycans that are characteristic of extreme human longevity.Methods
Plasma proteins from Japanese semi-supercentenarians (SSCs, 106–109?years), aged controls (70–88?years), and young controls (20–38?years) were analysed by using lectin microarrays and liquid chromatography/mass spectrometry (LC/MS). Peak area ratios of glycopeptides to corresponding normalising peptides were subjected to orthogonal projections to latent structures discriminant analysis (OPLS-DA). Furthermore, plasma levels of clinical biomarkers were measured.Results
We found two lectins such as Phaseolus vulgaris, and Erythrina cristagalli (ECA), of which protein binding were characteristically increased in SSCs. Peak area ratios of ECA-enriched glycopeptides were successfully discriminated between SSCs and controls using OPLS-DA, and indicated that tri-antennary and sialylated N-glycans of haptoglobin at Asn207 and Asn211 sites were characterized in SSCs. Sialylated glycans of haptoglobin are a potential biomarker of several diseases, such as hepatocellular carcinoma, liver cirrhosis, and IgA-nephritis. However, the SSCs analysed here did not suffer from these diseases.Conclusions
Tri-antennary and sialylated N-glycans on haptoglobin at the Asn207 and Asn211 sites were abundant in SSCs and characteristic of extreme human longevity.General significance
We found abundant glycans in SSCs, which may be associated with human longevity. 相似文献76.
Noritaka Matsuo Koji Yamada Kouhei Yamashita Kentaro Shoji Mitsuo Mori Michihiro Sugano 《In vitro cellular & developmental biology. Animal》1996,32(6):340-344
Summary The effect of tea polyphenols on the release of chemical mediators, histamine and leukotriene B4 (LTB4), from rat peritoneal exudate cells (PEC) was studied. Among polyphenols, (−)-epigallocatechin gallate (EGCG) most strongly
inhibited the histamine release from the cells stimulated with a calcium ionophore, A23187 or compound 48/80. Though (+)-catechin
(C) and (−)-epicatechin (EC) had no effect, (−)-epigallocatechin (EGC) and (−)-epicatechin gallate (ECG) moderately inhibited
the histamine release. Similarly, EGCG, ECG, and EGC inhibited LTB4 release from PEC, whereas C and EC were not effective. The magnitude of the inhibitory effect on the release of these mediators
of tea polyphenols was in the order of EGCG>ECG>EGC. These results indicated an important role of the triphenol structure
in the inhibitory activity. Therefore, the possible antiallergic effect of tea polyphenols can be expected. 相似文献
77.
78.
Hiromi?Ito Hiroyuki?Kaji Akira?Togayachi Parastoo?Azadi Mayumi?Ishihara Rudolf?Geyer Christina?Galuska Hildegard?Geyer Kazuaki?Kakehi Mitsuhiro?Kinoshita Niclas?G.?Karlsson Chunsheng?Jin Koichi?Kato Hirokazu?Yagi Sachiko?Kondo Nana?Kawasaki Noritaka?Hashii Daniel?Kolarich Kathrin?Stavenhagen Nicolle?H.?Packer Morten?Thaysen-Andersen Miyako?Nakano Naoyuki?Taniguchi Ayako?Kurimoto Yoshinao?Wada Michiko?Tajiri Pengyuan?Yang Weiqian?Cao Hong?Li Pauline?M.?RuddEmail author Hisashi?Narimatsu 《Glycoconjugate journal》2016,33(3):405-415
The Human Disease Glycomics/Proteome Initiative (HGPI) is an activity in the Human Proteome Organization (HUPO) supported by leading researchers from international institutes and aims at development of disease-related glycomics/glycoproteomics analysis techniques. Since 2004, the initiative has conducted three pilot studies. The first two were N- and O-glycan analyses of purified transferrin and immunoglobulin-G and assessed the most appropriate analytical approach employed at the time. This paper describes the third study, which was conducted to compare different approaches for quantitation of N- and O-linked glycans attached to proteins in crude biological samples. The preliminary analysis on cell pellets resulted in wildly varied glycan profiles, which was probably the consequence of variations in the pre-processing sample preparation methodologies. However, the reproducibility of the data was not improved dramatically in the subsequent analysis on cell lysate fractions prepared in a specified method by one lab. The study demonstrated the difficulty of carrying out a complete analysis of the glycome in crude samples by any single technology and the importance of rigorous optimization of the course of analysis from preprocessing to data interpretation. It suggests that another collaborative study employing the latest technologies in this rapidly evolving field will help to realize the requirements of carrying out the large-scale analysis of glycoproteins in complex cell samples. 相似文献
79.
Takeshi Takarada Masato Ogura Noritaka Nakamichi Takami Kakuda Ryota Nakazato Hiroshi Kokubo Shinsuke Ikeno Saki Nakamura Takaya Kutsukake Eiichi Hinoi Yukio Yoneda 《Neurochemical research》2016,41(1-2):5-15
We have shown marked promotion of both cluster growth and neuronal specification in pluripotent P19 cells with overexpression of solute carrier 38a1 (Slc38a1), which is responsible for membrane transport of glutamine. In this study, we evaluated pharmacological profiles of the green tea amino acid ingredient theanine, which is a good substrate for glutamine transporters, on proliferation and neuronal specification in neural progenitor cells from embryonic rat neocortex. Sustained exposure to theanine, but not glutamine, accelerated the growth of neurospheres composed of proliferating cells and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) reducing activity at concentrations of 1–100 μM in undifferentiated progenitor cells. Such prior exposure to theanine promoted spontaneous and induced commitment to a neuronal lineage with concomitant deteriorated astroglial specification. Selective upregulation was seen in the expression of Slc38a1 in progenitor cells cultured with theanine. Similarly significant increases in cluster growth and MTT reducing activity were found in P19 cells cultured with theanine for 4 days. Luciferase activity was doubled in a manner sensitive to the deletion of promoter regions in P19 cells with a luciferase reporter plasmid of the Slc38a1 promoter after sustained exposure to theanine for 4 days. Overexpression of X-box binding protein-1 led to a marked increase in luciferase activity in P19 cells transfected with the Slc38a1 reporter plasmid. These results suggest that theanine accelerates cellular proliferation and subsequent neuronal specification through a mechanism relevant to upregulation of Slc38a1 gene in undifferentiated neural progenitor cells. 相似文献
80.