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171.
Yuji Kiyama Ayako M Watabe Norikazu Katayama Kazumasa Yokoyama Takeshi Inoue Shigeru Kakuta Yoichiro Iwakura Hisashi Umemori Takafumi Inoue Niall P Murphy Kouichi Hashimoto Masanobu Kano Toshiya Manabe Tadashi Yamamoto 《The EMBO journal》2009,28(23):3717-3729
Major depressive and bipolar disorders are serious illnesses that affect millions of people. Growing evidence implicates glutamate signalling in depression, though the molecular mechanism by which glutamate signalling regulates depression‐related behaviour remains unknown. In this study, we provide evidence suggesting that tyrosine phosphorylation of the NMDA receptor, an ionotropic glutamate receptor, contributes to depression‐related behaviour. The NR2A subunit of the NMDA receptor is tyrosine‐phosphorylated, with Tyr 1325 as its one of the major phosphorylation site. We have generated mice expressing mutant NR2A with a Tyr‐1325‐Phe mutation to prevent the phosphorylation of this site in vivo. The homozygous knock‐in mice show antidepressant‐like behaviour in the tail suspension test and in the forced swim test. In the striatum of the knock‐in mice, DARPP‐32 phosphorylation at Thr 34, which is important for the regulation of depression‐related behaviour, is increased. We also show that the Tyr 1325 phosphorylation site is required for Src‐induced potentiation of the NMDA receptor channel in the striatum. These data argue that Tyr 1325 phosphorylation regulates NMDA receptor channel properties and the NMDA receptor‐mediated downstream signalling to modulate depression‐related behaviour. 相似文献
172.
Takeshi Taniguchi Ryota Kataoka Shigenobu Tamai Norikazu Yamanaka Kazuyoshi Futai 《Mycorrhiza》2009,19(4):231-238
The nitrogen-fixing tree black locust (Robinia pseudoacacia L.) seems to affect ectomycorrhizal (ECM) colonization and disease severity of Japanese black pine (Pinus thunbergii Parl.) seedlings. We examined the effect of black locust on the distribution of ECM and pathogenic fungi in soil. DNA was
extracted from soil at depths of 0–5 and 5–10 cm, collected from the border between a Japanese black pine- and a black locust-dominated
forest, and the distribution of these fungi was investigated by denaturing gradient gel electrophoresis. The effect of soil
nutrition and pH on fungal distribution was also examined. Tomentella sp. 1 and Tomentella sp. 2 were not detected from some subplots in the Japanese black pine-dominated forest. Ectomycorrhizas formed by Tomentella spp. were dominant in black locust-dominated subplots and very little in the Japanese black pine-dominated forest. Therefore,
the distribution may be influenced by the distribution of inoculum potential, although we could not detect significant relationships
between the distribution of Tomentella spp. on pine seedlings and in soils. The other ECM fungi were detected in soils in subplots where the ECM fungi was not detected
on pine seedlings, and there was no significant correlation between the distribution of the ECM fungi on pine seedlings and
in soils. Therefore, inoculum potential seemed to not always influence the ECM community on roots. The distribution of Lactarius quieticolor and Tomentella sp. 2 in soil at a depth of 0–5 cm positively correlated with soil phosphate (soil P) and that of Tomentella sp. 2 also positively correlated with soil nitrogen (soil N). These results suggest the possibility that the distribution
of inoculum potential of the ECM fungi was affected by soil N and soil P. Although the mortality of the pine seedlings was
higher in the black locust-dominated area than in the Japanese black pine-dominated area, a pathogenic fungus of pine seedlings,
Cylindrocladium pacificum, was detected in soil at depths of 0–5 and 5–10 cm from both these areas. This indicates that the disease severity of pine
seedlings in this study was influenced by environmental conditions rather than the distribution of inoculum potential. 相似文献
173.
Makoto Ando Etsuko Sekino Yuji Haga Minoru Moriya Masahiko Ito Junko Ito Hisashi Iwaasa Akane Ishihara Akio Kanatani Norikazu Ohtake 《Bioorganic & medicinal chemistry letters》2009,19(17):5186-5190
Novel phenethylpyridone derivatives were identified as potent human melanin-concentrating hormone 1 receptor (MCH-1R) antagonists. A search for surrogates for the 4-(2-aminoethoxy)phenyl moiety of 1 resulted in discovery of 2-[4-(aminomethyl)phenyl]ethyl substructure as in 6a. Successive optimization of the right-hand moiety led to the identification of a number of potent derivatives. 相似文献
174.
Norikazu Kiguchi Yuka Kobayashi Takehiko Maeda Shinta Tominaga Jun Nakamura Yohji Fukazawa Masanobu Ozaki Shiroh Kishioka 《Neurochemistry international》2012
Emerging evidence indicates that chronic neuroinflammation plays a pivotal role in neuropathic pain. We explored whether activation of the nicotinic acetylcholine receptor (nAChRs) pathway on peripheral immune cells improves neuropathic pain. Mice were subjected to partial sciatic nerve ligation (PSL). Enhanced green fluorescent protein (EGFP)-chimeric mice were generated by transplantation of EGFP+ bone marrow (BM) cells from EGFP-transgenic mice into wild-type mice. 相似文献
175.
Sakoda Y Okamatsu M Isoda N Yamamoto N Ozaki K Umeda Y Aoyama S Kida H 《Microbiology and immunology》2012,56(7):490-495
Affinity chromatography using sulfated, spherical cellulose beads (Cellufine Sulfate) was assessed for purification of influenza A and influenza B viruses. Recovery rates of viruses eluted from the beads were high for all tested virus strains. This method was also useful for removing chicken egg-derived impurities from allantoic fluids containing influenza viruses; the hemagglutination activity per amount of protein in the eluted sample was significantly higher than that in the applied sample. These results suggest that use of Cellufine Sulfate is a practical method for primary purification of influenza viruses in the process of influenza vaccine production. 相似文献
176.
Md. Ashraful Hoque Toru Arai Norikazu Nishino Hyun-Jung Kim Akihiro Ito Minoru Yoshida 《Bioorganic & medicinal chemistry letters》2012,22(21):6770-6772
Two thioacetate tails were introduced to the chlamydocin- and CHAP31-related cyclic tetrapeptides. An intramolecular disulfide bridge could be formed in the CHAP31-related cyclic peptides. Both the thioacetate-tailed and disulfide-bridged peptides were potent histone deacetylase inhibitors in the presence of sulfhydryl compound. Potent p21 promoter inducing activity was also observed in vivo. 相似文献
177.
Dawei Huang Xiaohui Li Lei Sun Zhilong Xiu Norikazu Nishino 《Journal of peptide science》2012,18(4):242-251
Histone deacetylase inhibitors (HDACIs) are a promising class of anticancer agents. To examine whether a slight change in the recognition domain could alter their inhibitory activity, we synthesized a series of cyclo(?l ‐Am7(S2Py)‐Aib‐l ‐Phe(n‐Me)‐d ‐Pro)derivatives and evaluated their HDAC inhibitory and anticancer activities. The peptides exhibited potent HDAC inhibitory activity and inhibited three human cancer cell lines with IC50 in the micromolar range. Docking and molecular dynamics simulation were conducted to explore the interaction mechanisms of class I and II HDACs with these inhibitors. It revealed that the zinc ion in the active site coordinated five atoms of HDACs and the sulfur atom of the inhibitor. The metal binding domains of these compounds interacted with HDAC2, and the surface recognition domains of these compounds interacted with HDAC4 through hydrogen bonding. The hydrophobic interactions also provided favorable contributions to stabilize the complexes. The results obtained from this study would be helpful for us to design some novel cyclic tetrapeptides that may act as potent HDACIs. Copyright © 2012 European Peptide Society and John Wiley & Sons, Ltd. 相似文献
178.
Noriyuki Nishioka Ken-ichi Inoue Kenjiro Adachi Hiroshi Kiyonari Mitsunori Ota Amy Ralston Norikazu Yabuta Shino Hirahara Robert O. Stephenson Narumi Ogonuki Ryosuke Makita Hiroki Kurihara Elizabeth M. Morin-Kensicki Hiroshi Nojima Janet Rossant Kazuki Nakao Hitoshi Niwa Hiroshi Sasaki 《Developmental cell》2009,16(3):398-410
179.
Naka T Fujiwara N Yano I Maeda S Doe M Minamino M Ikeda N Kato Y Watabe K Kumazawa Y Tomiyasu I Kobayashi K 《Biochimica et biophysica acta》2003,1635(2-3):83-92
The unique feature of the genus Sphingobacterium is the presence of sphingophospholipids and ceramides, besides diacylglycerophospholipids. As major cellular lipid components, five kinds of sphingophospholipids were purified from Sphingobacterium spiritivorum ATCC 33861(T), the type species of genus Sphingobacterium. They were identified as ceramide phosphorylethanolamines (CerPE-1 and CerPE-2), ceramide phosphoryl-myo-inositols (CerPI-1 and CerPI-2), and ceramide phosphorylmannose (CerPM-1). The ceramide of CerPE-1, CerPI-1, and CerPM-1 was composed of 15-methylhexadecasphinganine (isoheptadeca sphinganine, iso-C17:0) and 13-methyltetradecanoic acid (isopentadecanoic acid, iso-C15:0), whereas that of CerPE-2 and CerPI-2 was composed of isoheptadeca sphinganine and 2-hydroxy-13-methyltetradecanoic acid (2-hydroxy isopentadecanoic acid, 2-OH iso-C15:0). These sphingophospholipids were also found in cellular lipids of Sphingobacterium multivorum ATCC 33613(T), Sphingobacterium mizutaii ATCC 33299(T), Sphingobacterium faecium IFO 15299(T), Sphingobacterium thalpophilum ATCC 43320(T), and Sphingobacterium antarcticum ATCC 51969(T). To our knowledge, the existence of CerPM-1 is a novel sphingophospholipid through eukaryotic and prokaryotic cells. 相似文献
180.
Functional human T lymphocyte development from cord blood CD34+ cells in nonobese diabetic/Shi-scid,IL-2 receptor gamma null mice 总被引:8,自引:0,他引:8
Yahata T Ando K Nakamura Y Ueyama Y Shimamura K Tamaoki N Kato S Hotta T 《Journal of immunology (Baltimore, Md. : 1950)》2002,169(1):204-209
An experimental model for human T lymphocyte development from hemopoietic stem cells is necessary to study the complex processes of T cell differentiation in vivo. In this study, we report a newly developed nonobese diabetic (NOD)/Shi-scid, IL-2Rgamma null (NOD/SCID/gamma(c)(null)) mouse model for human T lymphopoiesis. When these mice were transplanted with human cord blood CD34(+) cells, the mice reproductively developed human T cells in their thymus and migrated into peripheral lymphoid organs. Furthermore, these T cells bear polyclonal TCR-alphabeta, and respond not only to mitogenic stimuli, such as PHA and IL-2, but to allogenic human cells. These results indicate that functional human T lymphocytes can be reconstituted from CD34(+) cells in NOD/SCID/gamma(c)(null) mice. This newly developed mouse model is expected to become a useful tool for the analysis of human T lymphopoiesis and immune response, and an animal model for studying T lymphotropic viral infections, such as HIV. 相似文献