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排序方式: 共有260条查询结果,搜索用时 281 毫秒
71.
Mohammad Faiyaz ul Haque Siegfried Uhlhaas Michael Knapp Herdit Schüler Waltraut Friedl Mahmud Ahmad Peter Propping 《Human genetics》1993,91(1):17-19
A large inbred kindred from Pakistan in which an isolated type of split-hand/split-foot anomaly is transmitted as an X-chromosomal trait has previously been described. An X/autosomal translocation and an X-chromosomal rearrangement have been excluded by cytogenetic studies. In order to map the gene responsible for this disorder, linkage analysis has been performed by using 14 highly polymorphic DNA markers distributed over the whole X chromosome. Two-point linkage analysis between the disease locus and X-chromosomal marker loci gives maximal lod scores at = 0.00 with the loci DXS294 (Z
max= 5.13) and HPRT (Z
max= 4.43), respectively, suggesting that the gene for the X-chromosomal split-hand/split-foot anomaly is localized at Xq26–q26.1. 相似文献
72.
May Poh Yik Goh Ajmal Faiz Kamaluddin Terence Jit Loong Tan Hartini Yasin Hussein Taha Abdalla Jama Norhayati Ahmad 《Saudi Journal of Biological Sciences》2022,29(1):304
Litsea elliptica is traditionally believed to prevent and treat stomach ulcers, cancer, fever and headaches. This study investigates the phytochemical composition, antioxidant and cytotoxic effects of L. elliptica leaf extracts. The phytochemical content was determined via GCMS analysis and total phenolic content (TPC) and total flavonoid content (TFC) were analysed using the Folin-Ciocalteu and aluminium-chloride assays. Antioxidant activities were determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging and ferric-ion reducing antioxidant power (FRAP) assays, whereas cytotoxicity was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and calcein/ethidium viability assays. The mechanism of cytotoxicity was investigated using Annexin V/propidium iodide. Modifications in the mitochondria were investigated using MitoTracker Red CMXRos. Ten and twenty-six compounds were characterized in the young-leaf and mixed-leaves extracts, respectively. The young-leaf methanolic extract demonstrated the highest antioxidant capacity of at least four-folds greater than the mixed-leaves and ethanolic extracts. The methanolic extract also had higher TPC and TFC values compared to the ethanolic extract. Although the mixed L. elliptica leaves had lower antioxidant capacities compared to the young leaves, the mixed leaves extract has demonstrated greater cytotoxicity against the A549 cancer cell line. Further investigation revealed that the L. elliptica leaves-induced cytotoxicity on A549 cells was possibly via the non-inflammatory mitochondria-mediated apoptotic pathway. Overall, our results showed the potential of the L. elliptica leaves possessing cytotoxic activities against carcinoma cells where the compounds present can be further investigated for its therapeutic application.Keyword: Litsea elliptica, Phytochemical composition, Antioxidant, Cytotoxicity, A549 cells, Anticancer 相似文献
73.
Katayoon Pakravan Ehsan Razmara Bashdar Mahmud Hussen Fatemeh Sattarikia Majid Sadeghizadeh Sadegh Babashah 《Journal of cellular and molecular medicine》2022,26(1):1
Different cellular and molecular mechanisms contribute to chondrocyte and osteocyte development. Although vital roles of the mothers against decapentaplegic homolog 4 (also called ‘SMAD4’) have been discussed in different cancers and stem cell‐related studies, there are a few reviews summarizing the roles of this protein in the skeletal development and bone homeostasis. In order to fill this gap, we discuss the critical roles of SMAD4 in the skeletal development. To this end, we review the different signalling pathways and also how SMAD4 defines stem cell features. We also elaborate how the epigenetic factors—ie DNA methylation, histone modifications and noncoding RNAs—make a contribution to the chondrocyte and osteocyte development. To better grasp the important roles of SMAD4 in the cartilage and bone development, we also review the genotype‐phenotype correlation in animal models. This review helps us to understand the importance of the SMAD4 in the chondrocyte and bone development and the potential applications for therapeutic goals. 相似文献
74.
Muhammad Shaiq Ali Shaukat Mahmud Shaista Perveen Viqar Uddin Ahmad Ghazala Hafeez Rizwani 《Phytochemistry》1999,50(8):441
The ethanolic extract of the fresh leaves of Calophyllum inophyllum afforded a pair of new epimers named as inophynone and isoinophynone. Their structures were elucidated with the aid of spectroscopic techniques. Some known constituents, cholesterol, friedelin, canophyllol and canophyllic acid, were also isolated from the same source. 相似文献
75.
Ianoul A Grant DD Rouleau Y Bani-Yaghoub M Johnston LJ Pezacki JP 《Nature chemical biology》2005,1(4):196-202
The contraction of cardiac myocytes is initiated by ligand binding to adrenergic receptors contained in nanoscale multiprotein complexes called signalosomes. The composition and number of functional signalosomes within cardiac myocytes defines the molecular basis of the response to adrenergic stimuli. For the first time, we demonstrated the ability of near-field scanning optical microscopy to visualize beta-adrenergic receptors at the nanoscale in situ. On H9C2 cells, mouse neonatal and mouse embryonic cardiac myocytes, we showed that functional receptors are organized into multiprotein domains of approximately 140 nm average diameter. Colocalization experiments in primary cells at the nanometer scale showed that 15-20% of receptors were preassociated in caveolae. These nanoscale complexes were sufficient to effect changes in ligand-induced contraction rate without the requirement for substantial changes in receptor distribution in the cellular membrane. Using fluorescence intensities associated with these nanodomains, we estimated the receptor density within the observed nanometer features and established a lower limit for the number of receptors in the signalosome. 相似文献
76.
Forton DM Karayiannis P Mahmud N Taylor-Robinson SD Thomas HC 《Journal of virology》2004,78(10):5170-5183
77.
78.
Interaction with coxsackievirus and adenovirus receptor, but not with decay-accelerating factor (DAF), induces A-particle formation in a DAF-binding coxsackievirus B3 isolate 总被引:1,自引:1,他引:0
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Milstone AM Petrella J Sanchez MD Mahmud M Whitbeck JC Bergelson JM 《Journal of virology》2005,79(1):655-660
Although many coxsackie B viruses interact with decay accelerating factor (DAF), attachment to DAF by itself is not sufficient to initiate infection. We examined the early events in infection that follow virus interaction with DAF, and with the coxsackievirus and adenovirus receptor (CAR). Interaction with soluble CAR in a cell-free system, or with CAR on the surfaces of transfected cells, induced the formation of A particles; interaction with soluble or cell surface DAF did not. The results suggest that CAR, but not DAF, is capable of initiating the conformational changes in the viral capsid that lead to release of viral nucleic acid. 相似文献
79.
80.
Arafat Rahman Oany Md Shahabuddin Ahmed Nasreen Jahan Md Abdul Latif Shahin Mahmud Md. Ahmed Hossain Fatema Akter Hasibul Haque Rakib Md. Shariful Islam 《Bioinformation》2015,11(11):493-500
Streptomyces xinghaiensis is a Gram-positive, aerobic and non-motile bacterium. The bacterial genome is known. Therefore, it is of
interest to study the uncharacterized proteins in the genome. An uncharacterized protein (gi|518540893|86 residues) in the
genome was selected for a comprehensive computational sequence-structure-function analysis using available data and tools. Subcellular
localization of the targeted protein with conserved residues and assigned secondary structures is documented. Sequence
homology search against the protein data bank (PDB) and non-redundant GenBank proteins using BLASTp showed different
homologous proteins with known antitoxin function. A homology model of the target protein was developed using a known
template (PDB ID: 3CTO:A) with 62% sequence similarity in HHpred after assessment using programs PROCHECK and QMEAN6.
The predicted active site using CASTp is analyzed for assigned anti-toxin function. This information finds specific utility in
annotating the said uncharacterized protein in the bacterial genome. 相似文献