全文获取类型
收费全文 | 2753篇 |
免费 | 180篇 |
国内免费 | 3篇 |
出版年
2023年 | 10篇 |
2022年 | 26篇 |
2021年 | 37篇 |
2020年 | 17篇 |
2019年 | 31篇 |
2018年 | 32篇 |
2017年 | 28篇 |
2016年 | 55篇 |
2015年 | 100篇 |
2014年 | 92篇 |
2013年 | 150篇 |
2012年 | 213篇 |
2011年 | 201篇 |
2010年 | 126篇 |
2009年 | 106篇 |
2008年 | 152篇 |
2007年 | 194篇 |
2006年 | 161篇 |
2005年 | 143篇 |
2004年 | 158篇 |
2003年 | 169篇 |
2002年 | 146篇 |
2001年 | 22篇 |
2000年 | 12篇 |
1999年 | 30篇 |
1998年 | 32篇 |
1997年 | 32篇 |
1996年 | 25篇 |
1995年 | 28篇 |
1994年 | 25篇 |
1993年 | 22篇 |
1992年 | 27篇 |
1991年 | 21篇 |
1990年 | 19篇 |
1989年 | 18篇 |
1988年 | 11篇 |
1987年 | 14篇 |
1986年 | 17篇 |
1985年 | 22篇 |
1984年 | 20篇 |
1983年 | 15篇 |
1982年 | 19篇 |
1981年 | 19篇 |
1980年 | 15篇 |
1979年 | 10篇 |
1977年 | 13篇 |
1975年 | 13篇 |
1974年 | 12篇 |
1969年 | 8篇 |
1968年 | 8篇 |
排序方式: 共有2936条查询结果,搜索用时 15 毫秒
941.
942.
943.
Rojas DA Moreira-Ramos S Zock-Emmenthal S Urbina F Contreras-Levicoy J Käufer NF Maldonado E 《The Journal of biological chemistry》2011,286(30):26480-26486
944.
In a bioinformatics based screen for chloroplast-localized protein kinases we noticed that available protein targeting predictors falsely predicted chloroplast localization. This seems to be due to interference with N-terminal protein acylation, which is of particular importance for protein kinases. Their N-myristoylation was found to be highly overrepresented in the proteome, whereas myristoylation motifs are almost absent in known chloroplast proteins. However, only abolishing their myristoylation was not sufficient to target those kinases to chloroplasts and resulted in nuclear accumulation instead. In contrast, inhibition of N-myristoylation of a calcium-dependent protein kinase was sufficient to alter its localization from the plasma membrane to chloroplasts and chloroplast localization of ferredoxin-NADP+ reductase and Rubisco activase could be efficiently suppressed by artificial introduction of myristoylation and palmitoylation sites. 相似文献
945.
To address the question of ligand entry process, we report targeted molecular dynamics simulations of the entry of the flexible ionic ligand GW0072 in the ligand binding domain of the nuclear receptor PPARγ. Starting with the ligand outside the receptor the simulations led to a ligand docked inside the binding pocket resulting in a structure very close to the holo-form of the complex. The results showed that entry process is guided by hydrophobic interactions and that entry pathways are very similar to exit pathways. We suggest that TMD method may help in discriminating between ligands generated by in silico docking. 相似文献
946.
Sharma R Sharma PR Kim YC Leitinger N Lee JK Fu SM Ju ST 《Journal of immunology (Baltimore, Md. : 1950)》2011,186(2):1268-1278
947.
Rohn JL Sims D Liu T Fedorova M Schöck F Dopie J Vartiainen MK Kiger AA Perrimon N Baum B 《The Journal of cell biology》2011,194(5):789-805
Although a large number of actin-binding proteins and their regulators have been identified through classical approaches, gaps in our knowledge remain. Here, we used genome-wide RNA interference as a systematic method to define metazoan actin regulators based on visual phenotype. Using comparative screens in cultured Drosophila and human cells, we generated phenotypic profiles for annotated actin regulators together with proteins bearing predicted actin-binding domains. These phenotypic clusters for the known metazoan "actinome" were used to identify putative new core actin regulators, together with a number of genes with conserved but poorly studied roles in the regulation of the actin cytoskeleton, several of which we studied in detail. This work suggests that although our search for new components of the core actin machinery is nearing saturation, regulation at the level of nuclear actin export, RNA splicing, ubiquitination, and other upstream processes remains an important but unexplored frontier of actin biology. 相似文献
948.
Gallová J Uhríková D Kučerka N Svorková M Funari SS Murugova TN Almásy L Mazúr M Balgavý P 《The Journal of membrane biology》2011,243(1-3):1-13
The influence of cholesterol and β-sitosterol on egg yolk phosphatidylcholine (EYPC) bilayers is compared. Different interactions of these sterols with EYPC bilayers were observed using X-ray diffraction. Cholesterol was miscible with EYPC in the studied concentration range (0-50 mol%), but crystallization of β-sitosterol in EYPC bilayers was observed at X ≥ 41 mol% as detected by X-ray diffraction. Moreover, the repeat distance (d) of the lamellar phase was similar upon addition of the two sterols up to mole fraction 17%, while for X ≥ 17 mol% it became higher in the presence of β-sitosterol compared to cholesterol. SANS data on suspensions of unilamellar vesicles showed that both cholesterol and β-sitosterol similarly increase the EYPC bilayer thickness. Cholesterol in amounts above 33 mol% decreased the interlamellar water layer thickness, probably due to "stiffening" of the bilayer. This effect was not manifested by β-sitosterol, in particular due to the lower solubility of β-sitosterol in EYPC bilayers. Applying the formalism of partial molecular areas, it is shown that the condensing effect of both sterols on the EYPC area at the lipid-water interface is small, if any. The parameters of ESR spectra of spin labels localized in different regions of the EYPC bilayer did not reveal any differences between the effects of cholesterol and β-sitosterol in the range of full miscibility. 相似文献
949.
Ruhl S Berlenbach P Langenfelder S Hörl D Lehn N Hiller KA Schmalz G Durchschlag H 《Applied and environmental microbiology》2011,77(3):749-755
Microbial contamination of whole human saliva is unwanted for certain in vitro applications, e.g., when utilizing it as a growth substratum for biofilm experiments. The aim of this investigation was to test gamma irradiation for its suitability to sterilize saliva and to investigate the treatment's influence on the composition and integrity of salivary proteins in comparison to filter sterilization. For inhibition of bacterial growth by gamma irradiation, a sterility assurance level of 10(-6) was determined to be reached at a dose of 3.5 kGy. At this dose, the integrity of proteins, as measured by fluorescence, circular dichroism, and gel electrophoretic banding pattern, and the enzymatic activities of salivary amylase and lysozyme were virtually unchanged. Filtration reduced the total protein concentration to about half of its original value and decreased lysozyme activity to about 10%. It can be concluded that irradiation is suitable for sterilizing whole saliva in its native form. 相似文献
950.