全文获取类型
收费全文 | 3434篇 |
免费 | 256篇 |
出版年
2021年 | 33篇 |
2020年 | 13篇 |
2019年 | 15篇 |
2018年 | 36篇 |
2017年 | 31篇 |
2016年 | 45篇 |
2015年 | 80篇 |
2014年 | 92篇 |
2013年 | 233篇 |
2012年 | 144篇 |
2011年 | 155篇 |
2010年 | 102篇 |
2009年 | 107篇 |
2008年 | 175篇 |
2007年 | 177篇 |
2006年 | 175篇 |
2005年 | 193篇 |
2004年 | 190篇 |
2003年 | 177篇 |
2002年 | 193篇 |
2001年 | 102篇 |
2000年 | 114篇 |
1999年 | 92篇 |
1998年 | 67篇 |
1997年 | 37篇 |
1996年 | 46篇 |
1995年 | 36篇 |
1994年 | 37篇 |
1993年 | 30篇 |
1992年 | 50篇 |
1991年 | 52篇 |
1990年 | 64篇 |
1989年 | 41篇 |
1988年 | 53篇 |
1987年 | 56篇 |
1986年 | 42篇 |
1985年 | 46篇 |
1984年 | 27篇 |
1983年 | 28篇 |
1982年 | 38篇 |
1981年 | 21篇 |
1980年 | 23篇 |
1979年 | 22篇 |
1978年 | 25篇 |
1977年 | 29篇 |
1976年 | 22篇 |
1974年 | 16篇 |
1973年 | 18篇 |
1971年 | 11篇 |
1968年 | 11篇 |
排序方式: 共有3690条查询结果,搜索用时 15 毫秒
41.
The phosphorylation of phosphoinositides in the acetylcholine receptor (AChR)-rich membranes from the electroplax of the electric fish Narke japonica has been examined. When the AChR-rich membranes were incubated with [gamma-32P]ATP, 32P was incorporated into only two inositol phospholipids, i.e., tri- and diphosphoinositide (TPI and DPI). Even after the alkali treatment of the membrane, AChR-rich membranes still showed a considerable DPI kinase activity upon addition of exogenous DPI. It is likely that the 32P-incorporation into these lipids was realized by the membrane-bound DPI kinase and phosphatidyl inositol (PI) kinase. Such a membrane-bound DPI kinase was activated by Ca2+ (greater than 10(-6) M), whereas the PI kinase appeared to be inhibited by Ca2+. The effect of Ca2+ on the DPI phosphorylation was further enhanced by the addition of ubiquitous Ca2+-dependent regulator protein calmodulin. Calmodulin antagonists such as chlorpromazine (CPZ), trifluoperazine (TFP), and N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7) inhibited the phosphorylation of DPI in the AChR-rich membranes. It is suggested that the small pool of TPI in the plasma membrane is replenished by such Ca2+- and calmodulin-dependent DPI kinase responding to the change in the intracellular Ca2+ level. 相似文献
42.
Direct-acting mutagenicity of N4-aminocytidine derivatives bearing alkyl groups at the hydrazino nitrogens. 总被引:1,自引:0,他引:1 下载免费PDF全文
To investigate the mechanism of N4-aminocytidine-induced mutagenesis, N'-alkyl-N4-aminocytidines and N4-alkyl-N4-aminocytidines were prepared and their mutagenicity on bacteria were assayed. N'-Methyl-N4-aminocytidine, N'-(2-hydroxyethyl)-N4-aminocytidine and N',N'-dimethyl-N4-aminocytidine showed direct-acting mutagenicity on S. typhimurium TA100 and E. coli WP2 uvrA, tester strains that are sensitive to base-pair substitutions. In contrast, N4-methyl-N4-aminocytidine, N4-(2-hydroxyethyl)-N4-aminocytidine and N4,N'-dimethyl-N4-aminocytidine were not mutagenic on these bacteria. Since N'-methyl-N4-aminocytidine does not form hydrazones, the possibility that N4-aminocytidine causes mutation due to its reactivity with carbonyl compounds has been excluded. Furthermore, the fact that only those alkyl N4-aminocytidines having a hydrogen on the nitrogen at position 4 are mutagenic is consistent with the previously proposed mechanism in which the tautomerization between the amino and the imino forms of N4-aminocytosine allowing an ambiguous base pairing is the cause of the mutagenesis. 相似文献
43.
44.
45.
Effect of Streptococcus faecalis BIO-4R on intestinal flora of weanling piglets and calves. 下载免费PDF全文
K Ozawa K Yabu-uchi K Yamanaka Y Yamashita S Nomura I Oku 《Applied microbiology》1983,45(5):1513-1518
The effect of oral administration of Streptococcus faecalis BIO-4R, an antibiotic-resistant lactic acid bacterium, on the intestinal flora of weanling piglets and cows reared on antibiotic-containing diet was investigated. Fourteen days after administration of the bacteria, the intestinal flora of the piglets was examined. Animals of the administered group had stabilized lactic flora such as bifidobacteria, streptococci, and lactobacilli, whereas most animals of control group had reduced lactic flora. On the other hand, abundant yeasts were detected from the cecum, colon, and feces of the control animals, but the levels were significantly lower in the animals given strain BIO-4R. The density of Salmonella in the intestine appeared to be reduced after the administration of strain BIO-4R. The number of BIO-4R cells was shown to be 10 times lower in the duodenum and jejunum than in the ileum, suggesting that strain BIO-4R might have grown transiently in the ileum. The similar trend toward stabilization of the lactic flora was also observed in cows after administration of BIO-4R. In addition, an antagonistic effect of the strain against yeasts and Salmonella was suggested. These findings indicate that the oral administration of strain BIO-4R is one of the useful methods whereby the potentially deleterious effect of antibiotics on the intestinal flora of farm animals may be minimized. 相似文献
46.
A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins 总被引:53,自引:0,他引:53
A temperature-sensitive E. coli mutant with a mutation in the spc ribosomal protein operon was found to have a conditional defect in the processing of precursor proteins destined for the periplasmic space or the outer membrane. At high temperatures, significant amounts of precursor proteins having unprocessed signal sequences are detected in the mutant cell by pulse-labeling. The precursors are processed at very slow rates during a subsequent chase. Genetic analysis indicates that the mutation impairs the function of a gene, termed secY, located at the promoter-distal part of the spc operon. The secY gene is distinct from those genes previously known to specify ribosomal proteins, yet it is within the spc operon. It is suggested that the product of the secY gene is a component of the cellular apparatus that is essential for protein secretion across the cytoplasmic membrane. The gene secY is probably identical with prlA, previously identified as a suppressor of signal sequence mutations. 相似文献
47.
48.
Axial changes in the nemertean egg and embryo during development and its phylogenetic significance 总被引:4,自引:0,他引:4
On the basis of the axial relationship between the larva and adult, it is possible to divide the larvae of the members of the Heteronemertea and a species of the Palaeonemertea into five types, forming a sequential series in which the adult axis of each advances by an angle of 45° from that immediately preceding it. Phylogenetic consideration of the Nemertea has been undertaken in the light of evidence provided by the axiality of the embryos and the developmental data hitherto recorded. 相似文献
49.
50.