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51.
Masana Noma Masao Noguchi Einosuke Tamaki 《Bioscience, biotechnology, and biochemistry》2013,77(7):1411-1412
Screening was carried out to obtain microorganisms which produced the enzyme to reduce the disulfide bond, from our type cultures of yeast. Among many strains of yeast showing activity to reduce the disulfide bond, Candida claussenii, Candida brumptii and Candida rugosa were selected to have the highest activity. The enzyme activity was detected in the cell free extracts, but not in culture broth.The highest enzyme formation occured during the exponential growth phase, and rapid decrease of activity was observed in the stationary phase. Pantothenate and boron ion contributed to enzyme formation, and biotin and zinc ion to growth. The maximum enzyme activity was obtained in the following synthetic medium: 10% sucrose, 0.3% (NH4)2SO4, 0.5% KH2PO4, 0.15% MgCl2·6HO2 0.05% CaCl2, 0.015% MnCl2, 0.001% pantothenate, 0.0001% biotin, 0.0001% H3BO3, 0.00004% FeCl3·6H2O and 0.00008% ZnCl2. In addition, 30°C of the cultural temperature and vigorous aeration showed good results for enzyme formation. 相似文献
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Akira Isogai Akinori Suzuki Shizuo Higashikawa Shimpei Kuyama Saburo Tamura 《Bioscience, biotechnology, and biochemistry》2013,77(4):1023-1024
The structure of the xyloglucan synthesised in vitro by the particulate fraction of suspension-cultured soybean (Glycine max) cells from UDP-glucose and UDP-xylose is mainly composed of two kinds of oligosaccharide-building blocks, a heptasaccharide unit and a pentassaccharide unit [T. Hayashi and K. Matsuda, J. Biol Chem., 256, 11117 (1981)]. The synthesis of the pentasaccharide unit is probably the first step in the construction of oligosaccharide building blocks to elongate the ²-1,4-glucan backbone. This enzymatically synthesized xyloglucan was shown to have the same molecular size (Mw, 180,000) as the xyloglucan prepared from soybean cell walls by gel filtration on a Sepharose CL-6B column, and the same building blocks distributed among each fraction. A pulse-chase experiment indicated that the pentasaccharide unit was converted into the heptasaccharide unit. The conversion was regulated by the concentration of UDP-xylose. 相似文献
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Nobutaka Takahashi Akinori Suzuki Satoshi Miyamoto Rinpei Mori Saburo Tamura 《Bioscience, biotechnology, and biochemistry》2013,77(8):583-589
Molecular formula C25H37–39NO4 was assigned to Piericidin A. Through the examination of the ultraviolet, infrared and n.m.r. spectra and of chemical evidences, it was shown that Piericidin A contains one secondary alcohol, one acidic hydroxyl group, two methoxyl groups, tri- and tetra-substituted conjugated dienes and one polysubstituted heteroaromatic ring. 相似文献
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Akira Isogai Akinori Suzuki Shizuo Higashkawa Shimpei Kuyama Saburo Tamura 《Bioscience, biotechnology, and biochemistry》2013,77(12):3033-3035
( + )-α-Kainic acid (1) was synthesized by starting from a building block, N-Boc-3-acetoxyallylglycine ethyl ester (2). The key intermediate, a methyl 4-[(tert-butoxycarbonyl)prenylamino]-5-hydroxy-2-pentenoate derivative (9), was prepared from 2 in eight synthetic steps. After converting 10 into a methyl ester (11), intramolecular ene-carbocyclization of 11 gave a pyrrolidine derivative (12), which was converted to 1 in a moderate yield. 相似文献
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Saburo Tamura Ching-Fun Chang Akinori Suzuki Sumio Kumai 《Bioscience, biotechnology, and biochemistry》2013,77(3):391-397
Two new isoflavonoids were isolated from red clover as germination inhibitors for the same plant and their structures were determined as a glucoside of biochanin A (7-d-β-glucosyl-5,7-dihydroxy-4′-methoxyisoflavone) (II) and its 5-malonate (I), respectively. Besides these compounds the following substances were also isolated as inhibitors: trifolirhizin (III), ononin (IV), daidzein (V) and its 7-glucoside (VI), formononetin (VII), genistein (VIII) and biochanin A (IX). 相似文献
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Vahbiz Jokhi James Ashley John Nunnari Akiko Noma Naoto Ito Noriko Wakabayashi-Ito Melissa J. Moore Vivian Budnik 《Cell reports》2013,3(4):988-995
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60.
Takao?MunemotoEmail author Akinori?Masuda Nobuatsu?Nagai Muneki?Tanaka Soejima?Yuji 《BioPsychoSocial medicine》2013,7(1):9