全文获取类型
收费全文 | 845篇 |
免费 | 71篇 |
出版年
2023年 | 15篇 |
2022年 | 20篇 |
2021年 | 33篇 |
2020年 | 16篇 |
2019年 | 16篇 |
2018年 | 22篇 |
2017年 | 26篇 |
2016年 | 21篇 |
2015年 | 52篇 |
2014年 | 56篇 |
2013年 | 46篇 |
2012年 | 73篇 |
2011年 | 75篇 |
2010年 | 45篇 |
2009年 | 25篇 |
2008年 | 31篇 |
2007年 | 37篇 |
2006年 | 36篇 |
2005年 | 31篇 |
2004年 | 21篇 |
2003年 | 31篇 |
2002年 | 25篇 |
2001年 | 25篇 |
2000年 | 20篇 |
1999年 | 13篇 |
1998年 | 11篇 |
1996年 | 7篇 |
1995年 | 2篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 6篇 |
1991年 | 2篇 |
1990年 | 8篇 |
1989年 | 8篇 |
1988年 | 4篇 |
1987年 | 5篇 |
1986年 | 4篇 |
1985年 | 5篇 |
1984年 | 2篇 |
1983年 | 4篇 |
1982年 | 2篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1976年 | 3篇 |
1975年 | 2篇 |
1974年 | 4篇 |
1973年 | 3篇 |
1972年 | 2篇 |
1960年 | 2篇 |
排序方式: 共有916条查询结果,搜索用时 15 毫秒
891.
892.
Phuc Van Pham Ngoc Bich Vu Thuy Thi-Thanh Dao Ha Thi-Ngan Le Lan Thi Phi Oanh Thuy Huynh Mai Thi-Hoang Truong Oanh Thi-Kieu Nguyen Ngoc Kim Phan 《Cytotechnology》2017,69(5):801-814
Ischemia are common conditions related to lack of blood supply to tissues. Depending on the ischemic sites, ischemia can cause different diseases, such as hindlimb ischemia, heart infarction and stroke. This study aims to evaluate how extracellular vesicles (EVs) derived from ETV2 transfected fibroblasts affect endothelial cell proliferation and neovascularization in a murine model of hindlimb ischemia. Human fibroblasts were isolated and cultured under standard conditions and expanded to the 3th passage before use in experiments. Human fibroblasts were transduced with a viral vector containing the ETV2 gene. Transduced cells were selected by puromycin treatment. These cells were further cultured for collection of EVs, which were isolated from culture supernatant. Following co-culture with endothelial cells, EVs were evaluated for their effect on endothelial cell proliferation and were directly injected into ischemic tissues of a murine model of hindlimb ischemia. The results showed that EVs could induce endothelial cell proliferation in vitro and improved neovascularization in a murine model of hindlimb ischemia. Our results suggest that EVs derived from ETV2-transfected fibroblasts can be promising non-cellular products for the regeneration of blood vessels. 相似文献
893.
894.
Pham Hai Yen Nguyen Huy Hoang Nguyen Xuan Nhiem Phan Thi Thanh Huong Duong Thi Dung Nguyen Thi Cuc Nguyen The Cuong Bui Van Thanh Bui Huu Tai Phan Van Kiem 《化学与生物多样性》2023,20(6):e202300372
From the fruits of Schisandra cauliflora, five new dimethylbutyrylated dibenzocyclooctadiene lignans, named schisandracaurins A−E, were isolated using separation and chromatographic techniques. Their structures were determined by extensive analyses of HR-ESI-MS, NMR, and ECD spectra. The schisandracaurins A−E potentially inhibited NO production in LPS-activated RAW264.7 cells with their IC50 values from 21.4 to 30.3 μM. 相似文献
895.
Xiaojian Yin Yaping Xiang Feng-Qing Huang Yahui Chen Hengwu Ding Jinfa Du Xiaojie Chen Xiaoxiao Wang Xinru Wei Yuan-Yuan Cai Wen Gao Dongshu Guo Raphael N. Alolga Xianzhao Kan Baolong Zhang Gerardo Alejo-Jacuinde Ping Li Lam-Son Phan Tran Luis Herrera-Estrella Xu Lu Lian-Wen Qi 《Plant biotechnology journal》2023,21(11):2209-2223
Lonicera macranthoides (LM) and L. japonica (LJ) are medicinal plants widely used in treating viral diseases, such as COVID-19. Although the two species are morphologically similar, their secondary metabolite profiles are significantly different. Here, metabolomics analysis showed that LM contained ~86.01 mg/g hederagenin-based saponins, 2000-fold higher than LJ. To gain molecular insights into its secondary metabolite production, a chromosome-level genome of LM was constructed, comprising 9 pseudo-chromosomes with 40 097 protein-encoding genes. Genome evolution analysis showed that LM and LJ were diverged 1.30–2.27 million years ago (MYA). The two plant species experienced a common whole-genome duplication event that occurred ∼53.9–55.2 MYA before speciation. Genes involved in hederagenin-based saponin biosynthesis were arranged in clusters on the chromosomes of LM and they were more highly expressed in LM than in LJ. Among them, oleanolic acid synthase (OAS) and UDP-glycosyltransferase 73 (UGT73) families were much more highly expressed in LM than in LJ. Specifically, LmOAS1 was identified to effectively catalyse the C-28 oxidation of β-Amyrin to form oleanolic acid, the precursor of hederagenin-based saponin. LmUGT73P1 was identified to catalyse cauloside A to produce α-hederin. We further identified the key amino acid residues of LmOAS1 and LmUGT73P1 for their enzymatic activities. Additionally, comparing with collinear genes in LJ, LmOAS1 and LmUGT73P1 had an interesting phenomenon of ‘neighbourhood replication’ in LM genome. Collectively, the genomic resource and candidate genes reported here set the foundation to fully reveal the genome evolution of the Lonicera genus and hederagenin-based saponin biosynthetic pathway. 相似文献
896.
Summary Meiotic chromosomes of antarctic krill, Euphausia superba Dana, collected at Admiralty Bay, are described and the method used to obtain this genetic material is reported. Forty seven dividing meiotic nuclei at diakinesis and metaphase I from seminal glands of four males were examined, all with n= 17 bivalents. The results were compared with those obtained by other workers using krill specimens from the opposite side of the Antarctic continent and apparent differences in chromosome numbers were observed. Possible reasons for these differences and the potential for using cytogenetic techniques to study populations of krill are discussed. 相似文献
897.
898.
Functional RNAs can fold into intricate structures using a number of different secondary and tertiary structural motifs. Many factors contribute to the overall free energy of the target fold. This study aims at quantifying the entropic costs coming from the loss of conformational freedom when the sugar-phosphate backbone is subjected to constraints imposed by secondary and tertiary contacts. Motivated by insights from topology theory, we design a diagrammatic scheme to represent different types of RNA structures so that constraints associated with a folded structure may be segregated into mutually independent subsets, enabling the total conformational entropy loss to be easily calculated as a sum of independent terms. We used high-throughput Monte Carlo simulations to simulate large ensembles of single-stranded RNA sequences in solution to validate the assumptions behind our diagrammatic scheme, examining the entropic costs for hairpin initiation and formation of many multiway junctions. Our diagrammatic scheme aids in the factorization of secondary/tertiary constraints into distinct topological classes and facilitates the discovery of interrelationships among multiple constraints on RNA folds. This perspective, which to our knowledge is novel, leads to useful insights into the inner workings of some functional RNA sequences, demonstrating how they might operate by transforming their structures among different topological classes. 相似文献
899.
Thao P Phan Aubrey L Maryniak Christina A Boatwright Junsu Lee Alisa Atkins Andrea Tijhuis Diana CJ Spierings Hisham Bazzi Floris Foijer Philip W Jordan Travis H Stracker Andrew J Holland 《The EMBO journal》2021,40(1)
Mutations in centrosome genes deplete neural progenitor cells (NPCs) during brain development, causing microcephaly. While NPC attrition is linked to TP53‐mediated cell death in several microcephaly models, how TP53 is activated remains unclear. In cultured cells, mitotic delays resulting from centrosome loss prevent the growth of unfit daughter cells by activating a pathway involving 53BP1, USP28, and TP53, termed the mitotic surveillance pathway. Whether this pathway is active in the developing brain is unknown. Here, we show that the depletion of centrosome proteins in NPCs prolongs mitosis and increases TP53‐mediated apoptosis. Cell death after a delayed mitosis was rescued by inactivation of the mitotic surveillance pathway. Moreover, 53BP1 or USP28 deletion restored NPC proliferation and brain size without correcting the upstream centrosome defects or extended mitosis. By contrast, microcephaly caused by the loss of the non‐centrosomal protein SMC5 is also TP53‐dependent but is not rescued by loss of 53BP1 or USP28. Thus, we propose that mutations in centrosome genes cause microcephaly by delaying mitosis and pathologically activating the mitotic surveillance pathway in the developing brain. 相似文献
900.