Mechanical Allodynia Circuitry in the Dorsal Horn Is Defined by the Nature of the Injury

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Alizarin glucosyl transferase activity inStreptomyces aureofaciens mutants

Alizarin glucosyl transferase activity was found in five mutant strains ofStreptomyces aureofaciens. The activity bears no direct relationship to the final products of tetracycline biosynthesis. Translated by K. Sigler     

Donor cell memory confers a metastable state of directly converted cells

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Radioimmunoassay for calcitonin gene-related peptide and its measurement in sera of patients with thyroid disease.

To investigate serum levels of calcitonin gene-related peptide (CGRP), we developed a sensitive radioimmunoassay (RIA). RIA for CGRP in serum can present some problems: the serum may degradate the tracer during incubation and suppress the antigen-antibody reaction. We avoided these problems by using aprotinin and CGRP-free serum instead of a buffer for the standard curve. We detected serum CGRP in all 39 healthy subjects when CGRP-free serum was not used for the standard curve, but 34 of these subjects had serum CGRP levels below the detection limit (less than 80 pmol/l) when CGRP-free serum was used for the standard curve. We defined the normal range for serum CGRP as below 100.8 pmol/l, which was the maximum level found in the healthy subjects. We studied serum levels of this peptide in patients with thyroid diseases, because the thyroid may be one origin of circulating CGRP. Four of 10 patients with medullary thyroid carcinoma had elevated serum levels of CGRP. Seven of 24 patients with subacute thyroiditis had elevated serum levels of CGRP, but at least one year after clinical recovery, CGRP was undetectable in all. Seven of the 37 patients with hypothyroidism had elevated serum levels of CGRP. None of the patients with hyperthyroidism, adenomatous goiter, thyroid adenoma, or thyroid carcinoma had elevated serum CGRP levels. It is necessary to use a standard curve obtained by the addition of aprotinin and CGRP-free serum to the assay standards to measure serum CGRP levels. Some patients with subacute thyroiditis, hypothyroidism, or medullary thyroid carcinoma had elevated serum CGRP levels.     

Over-Expression of Ephrin-A5 in Mice Results in Decreasing the Size of Progenitor Pool through Inducing Apoptosis

Eph receptors and their ligands, ephrins, mediate cell-to-cell contacts in a specific brain region and their bidirectional signaling is implicated in the regulation of apoptosis during early brain development. In this report, we used the alpha(α)-Cre transgenic line to induce ephrin-A5 over-expression in the distal region of the neural retina. Using this double transgenic embryo, we show that the over-expression of ephrin-A5 was responsible for inducing massive apoptosis in both the nasal and temporal retinas. In addition, the number of differentiated retinal neurons with the exception of the bipolar neuron was significantly reduced, whereas the laminar organization of the mature retina remained intact. Consistent with this finding, an analysis of the mature retina revealed that the size of the whole retina— particularly the nasal and temporal regions—is markedly reduced. These results strongly suggest that the level of ephrin-A5 expression plays a role in the regulation of the size of the retinal progenitor pool in the neural retina.     

New Species and Eight Undescribed Species Belonging to the Families Aspergillaceae and Trichocomaceae in Korea

During a survey of fungal diversity associated with insects, mud, soil, and freshwater niches in different areas in Korea, nine interesting fungal strains were isolated. Based on their morphological characteristics and molecular phylogeny analyses, using a combined data set of β-tubulin (BenA), calmodulin (CaM), and second largest subunit of RNA polymerase (RPB2) sequences, the strains CNUFC AM-44, CNUFC JCW3-4, CNUFC S708, CNUFC WT202, CNUFC AS1-29, CNUFC JCW3-5, CNUFC JDP37, and CNUFC JDP62 were identified as Aspergillus alabamensis, A. floridensis, A. subversicolor, Penicillium flavigenum, P. laevigatum, P. lenticrescens, Talaromyces adpressus, and T. beijingensis, respectively. The strain CNUFC JT1301 belongs to series Westlingiorum in section Citrina and is phylogenetically related to P. manginii. However, slow growth when cultivated on CYA, MEA, CREA is observed and the property can be used to easily distinguish the new species from these species. Additionally, P. manginii is known to produce sclerotia, while CNUFC JT1301 strain does not. Herein, the new fungal species is proposed as P. aquadulcis sp. nov. Eight species, A. alabamensis, A. floridensis, A. subversicolor, P. flavigenum, P. laevigatum, P. lenticrescens, T. adpressus, and T. beijingensis, have not been previously reported in Korea. The present study expands the known distribution of fungal species belonging to the families Aspergillaceae and Trichocomaceae in Korea.     

Atomic Layer Deposition of Tin Monosulfide Thin Films

Thin film solar cells made from earth‐abundant, non‐toxic materials are needed to replace the current technology that uses Cu(In,Ga)(S,Se)₂ and CdTe, which contain scarce and toxic elements. One promising candidate absorber material is tin monosulfide (SnS). In this report, pure, stoichiometric, single‐phase SnS films were obtained by atomic layer deposition (ALD) using the reaction of bis(N,N′‐diisopropylacetamidinato)tin(II) [Sn(MeC(N‐ⁱPr)₂)₂] and hydrogen sulfide (H₂S) at low temperatures (100 to 200 °C). The direct optical band gap of SnS is around 1.3 eV and strong optical absorption (α > 10⁴ cm^?1) is observed throughout the visible and near‐infrared spectral regions. The films are p‐type semiconductors with carrier concentration on the order of 10¹⁶ cm^?3 and hole mobility 0.82–15.3 cm²V^?1s^?1 in the plane of the films. The electrical properties are anisotropic, with three times higher mobility in the direction through the film, compared to the in‐plane direction.     

The Relationship between Sleep and Shift System,Age and Chronotype in Shift Workers

The purpose of this study was to investigate the effects of shift system, age, and chronotype on the sleep habits, sleepiness and catnaps of shift workers during night work. We administrated a Morningness-Eveningness Questionnaire and Life Habits Inventory to 561 male shift workers of three different shift systems in Korea. The mean scores on the Morningness-Eveningness Questionnaire clearly shifted toward the Morning type from the young to old groups. The waking and bedtimes during the day and evening shift were earlier for the older than for the younger groups, and these times were earlier across the Evening, Intermediate, to Morning type as well. Sleep length during the day shift was longer from the young to old, and it was shorter from the Evening, Intermediate, to Morning type. In the weekly rotation full-day 3-team 3-shift system and continuous full-day 3-team 3-shift system (which had earlier starting times for day shift) the sleep length of the younger workers was short. For the aged workers, sleep length during the night shift was short because they woke up early. In the weekly rotation full-day 2-team 2-shift system that had a short working interval, the reduction of sleep length during night shift was greater than that of the other shift systems. However, the scores on the Morningness-Eveningness Questionnaire showed a significantly negative correlation only in the bedtime during day shift among the sleep habit parameters for the independent variable by age. For the percentage of subjects that reported 'become sleepiness', the degree and time of sleepiness during the night shift were greater and earlier for the older than for the younger workers, and greater for the Morning than for the Evening type. The percentage of subjects who took a catnap during night shift, as well as the length of their catnap, were also higher and longer for the older groups, and higher and longer for the Morning type than for the Evening type. From these results, we surmised that the sleep habit parameters were influenced the age or shift systems rather than the chronotype. The sleep length during the night shift was shorter for the aged than for the young. In the shift systems that have a short working interval, the nocturnal was remarkably shorter for the young than the aged. The aged had not adapted their sleep for the night shift compared to the young, this suggested by the aged's strong complaint of sleepiness and by the fact that a larger percentage of aged subjects said they took catnaps during night shift compared to the younger workers.