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Cell surface human alpha-L-fucosidase. 总被引:1,自引:0,他引:1
O J Cordero A Merino M Páez de la Cadena B Bugía M Nogueira J E Vi?uela V S Martínez-Zorzano A de Carlos F J Rodríguez-Berrocal 《European journal of biochemistry》2001,268(11):3321-3331
The acid alpha-L-fucosidase is usually found as a soluble component of lysosomes where fucoglycoconjugates are degraded. In the present investigation, we have demonstrated the existence of a cell surface protein with enzymatic alpha-L-fucosidase activity that crossreacts specifically with a rabbit anti-(alpha-L-fucosidase) Ig. By different approaches, this alpha-L-fucosidase, which represents 10-20% of the total cellular fucosidase activity, was detected in all the tested human cells (hemopoietic, epithelial, mesenchymal). Two bands of approximately 43-49 kDa were observed, although theoretical data support the possibility of having the same genetic origin that the known 50 to 55-kDa Mr alpha-L-fucosidase. We speculate about an alternative traffic pathway for the plasma membrane alpha-L-fucosidase to work on the rapid turnover of glycoproteins. 相似文献
134.
Silvana Lú cio Nogueira Maria Bernardete Cordeiro de Sousa Ciro Franco de Medeiros Neto Maria da Paz de Oliveira Costa 《Biological Rhythm Research》2001,32(2):169-177
Chemical communication by scent-marking behavior in New World primates is used to prevent the access of potential competitors to a territory, to identify food resources and the reproductive condition of mates, among others. In common marmosets, primates of the Callitrichidae family, this behavior also occurs as olfactory identification of an individual or of the reproductive status of females. Despite this information, the diurnal variation and gender differences in the profile of this behavior remain to be investigated. The aims of this study were to establish the diurnal profile of the distribution of this behavior and the influence of the sex of markers. We used 18 adult common marmosets, Callithrix jacchus, 10 males and 8 females from 6 family groups (6 fathers and 4 sons; 4 mothers and 4 daughters). The frequency of scent-marking behavior was recorded for each animal over a period of 8 days, twice a week, for 4 weeks, starting when the animals left the nest box (approximately at 05:00 a.m.) until the end of the photophase, at about 05:00 p.m. A MANOVA test was performed to compare the frequency of scent-marking behavior at 2 hour intervals using pooled data for males and females. The results showed that significantly higher levels of scent-marking behavior occurred during the 03:00-05:00 p.m. interval compared to all other intervals. Lower values were recorded during the 11:00-13:00 interval and an effect of the sex factor was also found, with the values being higher for females than for males, although a significant difference was recorded only for the 07:00-09:00 interval. Minimal values for males were recorded during the 07:00-09:00 interval, whereas minimum values for females were recorded during the 11:00-13:00 interval. However, the highest values for both sexes continued to occur during the 15:00-17:00 interval. These results suggest that scent marking behavior in common marmosets has a preferential incidence at the end of the day and this might be occurring in association with feeding behavior. At this time these animals usually forage more to prepare for the night's fast. Since these animals can discriminate chemical clues as long as 24 hours after they have been left, the higher incidence of this behavior at this time probably will assure that the animals will localize feeding resources used on the preceding day. Significant elevation of scent marking behavior in females in relation to males was found only at 07:00-09:00 interval and seems to be associated with signalizing of reproductive status, preferential access to foraging or both. 相似文献
135.
Camila Simioni Vanzin Caroline Paula Mescka Bruna Donida Tatiane Grazieli Hammerschimidt Graziela S. Ribas Janaína Kolling Emilene B. Scherer Laura Vilarinho Célia Nogueira Adriana Simon Coitinho Moacir Wajner Angela T. S. Wyse Carmen Regla Vargas 《Cellular and molecular neurobiology》2015,35(6):899-911
136.
An outbreak of dengue virus at Rio de Janeiro--1986 总被引:1,自引:0,他引:1
H G Schatzmayr R M Nogueira A P Travassos da Rosa 《Memórias do Instituto Oswaldo Cruz》1986,81(2):245-246
Dengue virus type 1 has been isolated in Aedes albopictus cell strain, from sera of patients living in the Nova Igua?u county, by Rio de Janeiro. The clinical picture was characterized by fever, headache, retrobulbar pain, backache, pains in the muscles and the joints and prostration. Studies in paired sera confirmed the presence of recent infection by dengue virus type 1. The outbreak reached adjacent areas, including Rio de Janeiro city (May, 1986). 相似文献
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Fernanda P. Werneck Cristiano Nogueira Guarino R. Colli Jack W. Sites Jr Gabriel C. Costa 《Journal of Biogeography》2012,39(9):1695-1706
Aim To investigate the historical distribution of the Cerrado across Quaternary climatic fluctuations and to generate historical stability maps to test: (1) whether the ‘historical climate’ stability hypothesis explains squamate reptile richness in the Cerrado; and (2) the hypothesis of Pleistocene connections between savannas located north and south of Amazonia. Location The Cerrado, a savanna biome and a global biodiversity hotspot distributed mainly in central Brazil. Methods We generated occurrence datasets from 1000 presence points randomly selected from the entire distribution of the Cerrado, as determined by two spatial definitions. We modelled the potential Cerrado distribution by implementing a maximum‐entropy machine‐learning algorithm across four time projections: current, mid‐Holocene (6 ka), Last Glacial Maximum (LGM, 21 ka) and Last Interglacial (LIG, 120 ka). We generated historical stability maps (refugial areas) by overlapping presence/absence projections of all scenarios, and checked consistencies with qualitative comparisons with available fossil pollen records. We built a spatially explicit simultaneous autoregressive model to explore the relationship between current climate, climatic stability, and squamate species richness. Results Models predicted the LGM and LIG as the periods of narrowest and widest Cerrado distributions, respectively, and were largely corroborated by palynological evidence. We found evidence for two savanna corridors (eastern coastal during the LIG, and Andean during the LGM) and predicted a large refugial area in the north‐eastern Cerrado (Serra Geral de Goiás refugium). Variables related to climatic stability predicted squamate richness better than present climatic variables did. Main conclusions Our results indicate that Bolivian savannas should be included within the Cerrado range and that the Cerrado’s biogeographical counterparts are not Chaco and Caatinga but rather the disjunct savannas of the Guyana shield plateaus. Climatic stability is a good predictor of Cerrado squamate richness, and our stability maps could be used in future studies to test diversity patterns and genetic signatures of different taxonomic groups and as a higher‐order landscape biodiversity surrogate for conservation planning. 相似文献
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140.
Picoplankton community structure along the northern Iberian continental margin in late winter-early spring 总被引:1,自引:0,他引:1
Calvo-Diaz Alejandra; Moran Xose Anxelu G.; Nogueira Enrique; Bode Antonio; Varela Manuel 《Journal of plankton research》2004,26(9):1069-1081
The surface distribution of autotrophic and heterotrophic picoplanktonwas assessed in 24 transects perpendicular to the coast alongthe N and NW Iberian peninsula shelf in late winter and earlyspring 2002. Community structure was analyzed by flow cytometry(FC) and found to be strongly influenced by hydrography. Typicallate winter conditions were found during the survey, characterizedby the presence of the poleward Portugal coastal counter current(PCCC) in the west and an increasing stratification eastwards.Cyanobacteria (mostly Synechococcus) dominated at low chlorophylla (Chl a) concentration whereas both the total and relativeabundance of picoeukaryotes generally increased with total phytoplanktonbiomass. Differences in the cell size of most FC-defined picoplanktonicgroups were also observed along the longitudinal and coastaloffshoregradients. The presence of Prochlorococcus (<103 cells mL1)coincided with the core of the PCCC and its significant correlationwith salinity suggests its possible use as a tracer of thiscurrent. Two groups of heterotrophic bacteria were distinguishedaccording to their relative DNA content. High DNA bacteria dominatedthe community (60 ± 1% SE of total numbers), reachingmaximum values in areas under riverine influence with presumedhigher inputs of organic matter. Picoplankton biomass was dominatedby heterotrophic bacteria in the western region (58 ±3%) while autotrophic groups contributed on average 66 ±2% in the southern Bay of Biscay. The heterotrophic bacteriato phytoplankton biomass ratio decreased significantly alongthe measured range. Yet showing regional differences, the estimatedcontribution of picophytoplankton to total algal biomass washigh (mean 59 ± 4%), indicating the important role ofsmall cells at the onset of the spring bloom in these temperateshelf waters. 相似文献