全文获取类型
收费全文 | 2500篇 |
免费 | 146篇 |
国内免费 | 1篇 |
出版年
2022年 | 10篇 |
2021年 | 23篇 |
2020年 | 13篇 |
2019年 | 16篇 |
2018年 | 20篇 |
2017年 | 10篇 |
2016年 | 39篇 |
2015年 | 56篇 |
2014年 | 65篇 |
2013年 | 198篇 |
2012年 | 121篇 |
2011年 | 117篇 |
2010年 | 65篇 |
2009年 | 75篇 |
2008年 | 121篇 |
2007年 | 139篇 |
2006年 | 130篇 |
2005年 | 105篇 |
2004年 | 116篇 |
2003年 | 129篇 |
2002年 | 99篇 |
2001年 | 61篇 |
2000年 | 82篇 |
1999年 | 75篇 |
1998年 | 33篇 |
1997年 | 28篇 |
1996年 | 24篇 |
1995年 | 29篇 |
1994年 | 20篇 |
1993年 | 30篇 |
1992年 | 54篇 |
1991年 | 47篇 |
1990年 | 45篇 |
1989年 | 58篇 |
1988年 | 41篇 |
1987年 | 47篇 |
1986年 | 30篇 |
1985年 | 33篇 |
1984年 | 14篇 |
1983年 | 16篇 |
1982年 | 26篇 |
1981年 | 14篇 |
1980年 | 11篇 |
1979年 | 23篇 |
1978年 | 18篇 |
1977年 | 20篇 |
1976年 | 16篇 |
1975年 | 15篇 |
1973年 | 9篇 |
1971年 | 9篇 |
排序方式: 共有2647条查询结果,搜索用时 15 毫秒
991.
Kohji Yamamura 《Population Ecology》1999,41(3):229-234
Transformation is required to achieve homo-scedasticity when we perform ANOVA to test the effect of factors on population
abundance. The effectiveness of transformations decreases when the data contain zeros. Especially, the logarithmic transformation
or the Box–Cox transformation is not applicable in such a case. For the logarithmic transformation, 1 is traditionally added
to avoid such problems. However, there is no concrete foundation as to why 1 is added rather than other constants, such as
0.5 or 2, although the result of ANOVA is much influenced by the added constant. In this paper, I suggest that 0.5 is preferable
to 1 as an added constant, because a discrete distribution defined in {0, 1, 2, . . .} is approximately described by a corresponding
continuous distribution defined in (0, ≧) if we add 0.5. Numerical investigation confirms this prediction.
Received: October 16, 1998 / Accepted: June 10, 1999 相似文献
992.
Dephosphorylation of Microtubule Proteins by Brain Protein Phosphatases 1 and 2A, and Its Effect on Microtubule Assembly 总被引:4,自引:9,他引:4
Hideyuki Yamamoto Yoshiki Saitoh Kohji Fukunaga Hiroshi Nishimura Eishichi Miyamoto 《Journal of neurochemistry》1988,50(5):1614-1623
Protein phosphatase C was purified 140-fold from bovine brain with 8% yield using histone H1 phosphorylated by the catalytic subunit of cyclic AMP-dependent protein kinase (cyclic AMP-kinase). Brain protein phosphatase C was considered to consist of 10 and 90%, respectively, of the catalytic subunits of protein phosphatases 1 and 2A on the basis of the effects of ATP and inhibitor-2. Protein phosphatase C dephosphorylated microtubule-associated protein 2 (MAP2), tau factor, and tubulin phosphorylated by a multifunctional Ca2+/calmodulin-dependent protein kinase (calmodulin-kinase) and the catalytic subunit of cyclic AMP-kinase. The properties of dephosphorylation of MAP2, tau factor, and tubulin were compared. The Km values were in the ranges of 1.6-2.7 microM for MAP2 and tau factor. The Km value for tubulin decreased from 25 to 10-12.5 microM in the presence of 1.0 mM Mn2+. No difference in kinetic properties of dephosphorylation was observed between the substrates phosphorylated by the two kinases. Protein phosphatase C did not dephosphorylate the native tubulin, but universally dephosphorylated tubulin phosphorylated by the two kinases. The holoenzyme of protein phosphatase 2A from porcine brain could also dephosphorylate MAP2, tau factor, and tubulin phosphorylated by the two kinases. The phosphorylation of MAP2 and tau factor by calmodulin-kinase separately induced the inhibition of microtubule assembly, and the dephosphorylation by protein phosphatase C removed its inhibitory effect. These data suggest that brain protein phosphatases 1 and 2A are involved in the switch-off mechanism of both Ca2+/calmodulin-dependent and cyclic AMP-dependent regulation of microtubule formation. 相似文献
993.
994.
Noguchi E Sakamoto H Hirota T Ochiai K Imoto Y Sakashita M Kurosaka F Akasawa A Yoshihara S Kanno N Yamada Y Shimojo N Kohno Y Suzuki Y Kang MJ Kwon JW Hong SJ Inoue K Goto Y Yamashita F Asada T Hirose H Saito I Fujieda S Hizawa N Sakamoto T Masuko H Nakamura Y Nomura I Tamari M Arinami T Yoshida T Saito H Matsumoto K 《PLoS genetics》2011,7(7):e1002170
Asthma is a complex phenotype influenced by genetic and environmental factors. We conducted a genome-wide association study (GWAS) with 938 Japanese pediatric asthma patients and 2,376 controls. Single-nucleotide polymorphisms (SNPs) showing strong associations (P<1×10−8) in GWAS were further genotyped in an independent Japanese samples (818 cases and 1,032 controls) and in Korean samples (835 cases and 421 controls). SNP rs987870, located between HLA-DPA1 and HLA-DPB1, was consistently associated with pediatric asthma in 3 independent populations (P
combined = 2.3×10−10, odds ratio [OR] = 1.40). HLA-DP allele analysis showed that DPA1*0201 and DPB1*0901, which were in strong linkage disequilibrium, were strongly associated with pediatric asthma (DPA1*0201: P = 5.5×10−10, OR = 1.52, and DPB1*0901: P = 2.0×10−7, OR = 1.49). Our findings show that genetic variants in the HLA-DP locus are associated with the risk of pediatric asthma in Asian populations. 相似文献
995.
S Takahashi H Kato A Takahashi T Noguchi H Naito 《The International journal of biochemistry》1987,19(5):401-412
The chemical properties of acid soluble peptides accumulated in liver or excreted into urine after administration of bestatin or leupeptin to rats were investigated extensively. At the same time, the effects of glucagon on the bestatin-induced accumulation of acid soluble peptides were studied. The results show the important role of bestatin- and leupeptin-sensitive proteases in the degradation pathway of intracellular proteins in vivo. 相似文献
996.
Ken-ichi Kiyomiya Kohji Yamaki Noriyuki Nimura Toshio Kinoshita Sachiko Oh-ishi 《Prostaglandins & other lipid mediators》1986,31(1):71-82
White cells were collected from the wash of rat pleural cavity after exsanguination. The incubation mixture of the pleural cells with 1 μM phorbol myristate acetate (PMA) was extracted with acidified ethanol and purified with a Sep-pak C18. The resultant fraction containing prostaglandins (PG) and thromboxane (TX) was allowed to react with 9-anthryldiazomethane (ADAM). After removing contaminants and degraded reagent by silica gel Sep-pak, samples were applied to reversed phase high performance liquid chromatography of octadecylsilyl silica gel and monitored by a fluorescent detector. ADAM derivatives of the authentic PGD2, PGE2, PGF2α, 6-keto-PGF1α, 6-keto-PGE1, TXB2, 15-keo-PGE2, 13, 14-dihydro-15-keto-PGF2α and 13, 14-dihydro-15-keto-PGE2 showed linear regression lines of peak heights within a range of 0.5–25 ng.By using this method PGD2, 6-keto-PGF1α and TXB2 were detected in the incubation mixture of the rat pleural cells with PMA. The result clarified the origin of these PGs and TX found in the exudate of rat pleurisy induced by PMA.ADAM method for HPLC with a help of clean-up by Sep-pak could be a useful tool for detection of a series of arachidonate metabolites in biological materials. 相似文献
997.
Nagano K Taoka M Yamauchi Y Itagaki C Shinkawa T Nunomura K Okamura N Takahashi N Izumi T Isobe T 《Proteomics》2005,5(5):1346-1361
998.
Human M2-type pyruvate kinase: cDNA cloning, chromosomal assignment and expression in hepatoma 总被引:4,自引:0,他引:4
Two overlapping clones, covering the entire coding sequence of human M2-type pyruvate kinase (PK) cDNA, were isolated and sequenced. Nucleotide sequencing results showed that they contained the 109-bp 5'-untranslated region, the 1593-bp coding region and the 585-bp 3'-untranslated region. Nucleotide sequence homology was 90% and 69% with rat M2-type and L-type PK cDNA, respectively. In situ hybridization using the human M2-type PK cDNA probe disclosed that the gene for M2-type PK is located at band q22 on chromosome 15. Northern blot analysis with RNA from human hepatoma demonstrated that M2-type PK was predominantly expressed in hepatoma cells, whereas L-type PK was preferentially expressed in the non-tumor portion of the liver. 相似文献
999.
1000.
Tani H Limn CK Yap CC Onishi M Nozaki M Nishimune Y Okahashi N Kitagawa Y Watanabe R Mochizuki R Moriishi K Matsuura Y 《Journal of virology》2003,77(18):9799-9808
Although recombinant baculovirus vectors can be an efficient tool for gene transfer into mammalian cells in vitro, gene transduction in vivo has been hampered by the inactivation of baculoviruses by serum complement. Recombinant baculoviruses possessing excess envelope protein gp64 or other viral envelope proteins on the virion surface deliver foreign genes into a variety of mammalian cell lines more efficiently than the unmodified baculovirus. In this study, we examined the efficiency of gene transfer both in vitro and in vivo by recombinant baculoviruses possessing envelope proteins derived from either vesicular stomatitis virus (VSVG) or rabies virus. These recombinant viruses efficiently transferred reporter genes into neural cell lines, primary rat neural cells, and primary mouse osteal cells in vitro. The VSVG-modified baculovirus exhibited greater resistance to inactivation by animal sera than the unmodified baculovirus. A synthetic inhibitor of the complement activation pathway circumvented the serum inactivation of the unmodified baculovirus. Furthermore, the VSVG-modified baculovirus could transduce a reporter gene into the cerebral cortex and testis of mice by direct inoculation in vivo. These results suggest the possible use of the recombinant baculovirus vectors in combination with the administration of complement inhibitors for in vivo gene therapy. 相似文献