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321.
Noemi Jiménez-Rojo Jesús Sot Ana?R. Viguera M. Isabel Collado Alejandro Torrecillas J.C. Gómez-Fernández Félix?M. Go?i Alicia Alonso 《Biophysical journal》2014,106(12):2577-2584
Sphingosine [(2S, 3R, 4E)-2-amino-4-octadecen-1, 3-diol] is the most common sphingoid long chain base in sphingolipids. It is the precursor of important cell signaling molecules, such as ceramides. In the last decade it has been shown to act itself as a potent metabolic signaling molecule, by activating a number of protein kinases. Moreover, sphingosine has been found to permeabilize phospholipid bilayers, giving rise to vesicle leakage. The present contribution intends to analyze the mechanism by which this bioactive lipid induces vesicle contents release, and the effect of negatively charged bilayers in the release process. Fluorescence lifetime measurements and confocal fluorescence microscopy have been applied to observe the mechanism of sphingosine efflux from large and giant unilamellar vesicles; a graded-release efflux has been detected. Additionally, stopped-flow measurements have shown that the rate of vesicle permeabilization increases with sphingosine concentration. Because at the physiological pH sphingosine has a net positive charge, its interaction with negatively charged phospholipids (e.g., bilayers containing phosphatidic acid together with sphingomyelins, phosphatidylethanolamine, and cholesterol) gives rise to a release of vesicular contents, faster than with electrically neutral bilayers. Furthermore, phosphorous 31-NMR and x-ray data show the capacity of sphingosine to facilitate the formation of nonbilayer (cubic phase) intermediates in negatively charged membranes. The data might explain the pathogenesis of Niemann-Pick type C1 disease. 相似文献
322.
Life-history traits of batoids (Superorder Batoidea) in the Northeast Atlantic and the Mediterranean
Villagra Damian Van Bogaert Noemi Ampe Bart Walker Paddy Uhlmann Sven Sebastian 《Reviews in Fish Biology and Fisheries》2022,32(2):473-495
Reviews in Fish Biology and Fisheries - Life-history traits provide a way to estimate the vulnerability of both individuals and populations of a species to disturbance (e.g., overexploitation,... 相似文献
323.
Débora D. S. Vogel Nancy N. Ortiz-Villatoro Noemi S. Araújo Márcia Jonathas Guimarães Marques Flavio Aimbire Fúlvio A. Scorza Carla A. Scorza Regiane Albertini 《Journal of biophotonics》2021,14(6):e202000500
Stroke is the main cause of death and functional disability. The available therapy affects only 5% of patients, and new therapeutic approaches have been constantly tested. Transcranial photobiomodulation (PBM) is promising for its neuroprotective effect on brain injuries. Thus, the present study investigated the PBM effects in an in vivo model of ischemic stroke induced by photothrombosis (PT). Five different groups of Wistar rats were submitted or not to a daily dose of fish oil or/and laser sessions for 2 months. The ischemia volume was evaluated by stereology; GFAP, Iba and NeuN by immunohistochemistry; TNF-α, IL-1β, IL-6, IL-10 and TGF-β by ELISA assay. PBM influenced both the lesion volume and the GFAP. Furthermore, PBM and Ω-3 or both reduced Iba RNAm. PBM reduced TNF-α, IL-1β, IL-6, brain damage, neuroinflammation and microglial activation, and it increased astroglial activity in peri-lesioned region after stroke. 相似文献
324.
Noemi Correa Martina Alunni Cardinali Michelle Bailey Daniele Fioretto Paul D. A. Pudney Francesca Palombo 《Journal of biophotonics》2021,14(6):e202000483
Brillouin microscopy is a new form of optical elastography and an emerging technique in mechanobiology and biomedical physics. It was applied here to map the viscoelastic properties of human hair and to determine the effect of bleaching on hair properties. For hair samples, longitudinal measurements (i.e. along the fibre axis) revealed peaks at 18.7 and 20.7 GHz at the location of the cuticle and cortex, respectively. For hair treated with a bleaching agent, the frequency shifts for the cuticle and cortex were 19.7 and 21.0 GHz, respectively, suggesting that bleaching increases the cuticle modulus and—to a minor extent—the cortex modulus. These results demonstrate the capability of Brillouin spectroscopy to address questions on micromechanical properties of hair and to validate the effect of applied treatments. 相似文献
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