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471.
Molecular cloning, gene expression, and identification of a splicing variant of the mouse parkin gene 总被引:3,自引:0,他引:3
Tohru Kitada Shuichi Asakawa Shinsei Minoshima Yoshikuni Mizuno Nobuyoshi Shimizu 《Mammalian genome》2000,11(6):417-421
We have isolated mouse cDNA clones that are homologous to human Parkin gene, which was recently found to be responsible for
the pathogenesis of autosomal recessive juvenile parkinsonism (AR-JP). One of these cDNA clones had the 1,392-bp open reading
frame encoding a protein of 464 amino acids with presumed molecular weight of 51,615. The amino acid sequence of mouse parkin
protein exhibits 83.2% identity to human Parkin protein, including the ubiquitin-like domain at the N-terminus (identity =
89.5%) and the RING finger-like domain at the C-terminus (identity = 90.6%). Two other clones had the 783-bp open reading
frame encoding a truncated protein of 261 amino acids without RING finger-like domain. It was proved to be a novel splicing
variant by 3′-RACE method.
Northern blot analysis revealed that mouse parkin gene is expressed in various tissues including brain, heart, liver, skeletal muscle, kidney, and testis. It is notable that
mouse parkin gene expression appears evident in 15th day mouse embryo and increases toward the later stage of development. These mouse
parkin cDNA clones will be useful for elucidating the essential physiological function of parkin protein in mammals.
Received: 5 May 1999 / Accepted: 11 February 2000 相似文献
472.
Atsushi Suzuki Nobuyoshi Suzuki Yoshihide Ikeuchi Makoto Saito 《Bioscience, biotechnology, and biochemistry》2013,77(10):2467-2473
High hydrostatic pressures of 100 MPa to 300 MPa were applied to isolated myofibrils prepared from rabbit skeletal muscle to investigate the pressure-induced degradation of myofibrillar structure in the muscle.A marked loss of the regular structure was observed in the phase-contrast image of the isolated myofibrils pressurized at 150 MPa, with further progress of the rupture of structure with increasing pressure applied. When exposed to pressures of 200 MPa or higher, clumping of the crushed myofibrils was observed. Electron microscopic studies of the pressurized myofibrils showed that the loss of M-line materials, rupture of I-filament, and the loss of the structural continuity with the loss of Z-line progressed in the myofibrils with increasing pressure applied. A sigmoidal relationship was obtained between the degree of solubilization and the intensity of the pressure applied to the isolated myofibrils. The electrophoretic analysis indicated that the amount and the species of the protein released from the myofibrils at each stage of the pressurization corresponded to the disruption of the ultrastructure in the myofibrils. 相似文献
473.
Takeshi Imai Tatsuo Kurihara Nobuyoshi Esaki 《Bioscience, biotechnology, and biochemistry》2013,77(8):1376-1380
Selenite is a selenium source for selenoprotein biosynthesis in mammalian cells. Although previous studies have suggested the involvement of glutathione (GSH) and/or thioredoxin reductase in selenite metabolism, intracellular selenite metabolism remains largely unknown. Here, we report that GSH depletion did not affect the amount of selenoprotein in Hepa 1–6 cells, suggesting that GSH does not play a central role in the reduction of selenite in selenoprotein biosynthesis. On the other hand, we found that GSH is involved in the efflux of low-molecular-weight selenium compounds from cells, presumably via the formation of selenodiglutathione. Moreover, selenite inhibited the efflux of a fluorescent bimane-GS conjugate that is mediated by ATP-dependent multidrug-resistant proteins, implying the existence of an active transporter for selenodiglutathione. This is the first report demonstrating that GSH plays a role in selenium excretion from cells by forming a GSH-conjugate, which may contribute to the distribution, detoxification, and homeostasis of selenium in the body. 相似文献
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477.
Rachel?E. Pepper Marcus Roper Sangjin Ryu Nobuyoshi Matsumoto Moeto Nagai Howard?A. Stone 《Biophysical journal》2013,105(8):1796-1804
Microscopic sessile suspension feeders are a critical component in aquatic ecosystems, acting as an intermediate trophic stage between bacteria and higher eukaryotic taxa. Because they live attached to boundaries, it has long been thought that recirculation of the feeding currents produced by sessile suspension feeders inhibits their ability to access fresh fluid. However, previous models for the feeding flows of these organisms assume that they feed by pushing fluid perpendicular to surfaces they live upon, whereas we observe that sessile suspension feeders often feed at an angle to these boundaries. Using experiments and calculations, we show that living suspension feeders (Vorticella) likely actively regulate the angle that they feed relative to a substratum. We then use theory and simulations to show that angled feeding increases nutrient and particle uptake by reducing the reprocessing of depleted water. This work resolves an open question of how a key class of suspension-feeding organisms escapes physical limitations associated with their sessile lifestyle. 相似文献
478.
Kenzo Motosugi Nobuyoshi Esaki Kenji Soda 《Bioscience, biotechnology, and biochemistry》2013,77(3):837-838
Previous studies of potato varieties indicated that changes during cooking could be mathematically described and that some chemical components and the cell size may influence the cooking behavior. To find out whether the same principles can be adopted for other root vegetables, the cooking behavior of three other low-starch root vegetables were investigated and the results compared. Slices (6 mm thick and 30 mm diameter) were treated in water at 100°C. Mathematical expressions were assessed, and coefficients were determined to describe the kinetic behavior of the products. The cell size and pectin content of the raw materials determined the cooking characteristics. Texture development could be predicted by shear force measurements. 相似文献
479.
Kohji Ishihara Rieko Iwai Momoko Yoshida Mika Morishita Hitomi Yamaguchi Nobuyoshi Nakajima 《World journal of microbiology & biotechnology》2011,27(1):17-24
A novel keto ester reductase (Chlorella sorokiniana keto ester reductase, CSKER) from Chlorella sorokiniana SAG 211-8k cells was purified. The CSKER had a monomeric structure based on gel filtration chromatography (37 kDa) and SDS–polyacrylamide
gel electrophoresis (34 kDa). The purified CSKER showed a high reducing activity with β-keto esters, in particular, ethyl 4-chloro-3-oxobutanoate and ethyl 2-chloro-3-oxobutanoate. However, the purified enzyme
did not show any reducing activity with α-keto esters and 2-chlorobenzoylformamide (aromatic α-keto amide). The CSKER catalyzed
the reduction of ethyl 4-chloro-3-oxobutanoate, ethyl 3-oxobutanoate, and methyl 3-oxobutanoate to the corresponding (R)-, (S)-, and (S)-hydroxy ester, respectively, with high enantioselectivity (>99% e.e.), respectively. Furthermore, the reduction of ethyl
2-methyl-3-oxobutanoate by CSKER exclusively yielded the corresponding syn-(2R, 3S)-hydroxy ester. The purified CSKER was inactive with NADH, used instead of NADPH. None of the keto ester-reducing enzymes
already isolated from other microorganisms was identical to the CSKER. These results suggested that CSKER is a novel keto
ester reductase that has not yet been reported. 相似文献
480.
Shibue T Okamoto I Morita N Morita H Hirasawa Y Hosoya T Tamura O 《Bioorganic & medicinal chemistry letters》2011,21(1):431-434
The synthesis and biological evaluation of stereoisomers in tubulysin D are described. The stereoselective synthesis of all possible stereoisomers of C-11 and C-13 positions in tubulysin D was achieved by employing 1′-epi-Tuv-Me, 3′-epi-Tuv-Me, and ent-Tuv-Me and their biological properties were evaluated. It is clear that the stereochemistries of the C-11 and C-13 positions in tubulysin D have no practical impact on the inhibition of tubulin polymerization but play a role in the potent antiproliferative activities. 相似文献