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31.
Tsukasa Mizuhara Shinya Oishi Hiroaki Ohno Kazuya Shimura Masao Matsuoka Nobutaka Fujii 《Bioorganic & medicinal chemistry》2013,21(7):2079-2087
To investigate the mechanism of action of the potent antiviral compound PD 404182, three novel photoaffinity probes equipped with a biotin or alkyne indicator were designed and synthesized based on previous structure–activity relationship studies. These probes retained the potent anti-HIV activity of the original pyrimidobenzothiazine derivatives. In photoaffinity labeling studies using HIV-1-infected H9 cells (H9IIIB), eight potential proteins were observed to bind PD 404182. 相似文献
32.
Akira Sakurai Yukiharu Sato Keun Hyung Park Nobutaka Takahashi Naohiko Yanagishima Isao Banno 《Bioscience, biotechnology, and biochemistry》2013,77(6):1451-1453
The pullulanase gene (pul) of Klebsiella aerogenes was transferred in vivo to Escherichia coli by using RP4:: Mu cts. The pul gene was expressed in E. coli, although the level of pullulanase activity in E. coli was lower than that in K. aerogenes, and the Pul+ transconjugants were relatively unstable in an unselective medium. Production of pullulanase, which is used to make maltose from starch, was induced in E. coli by pullulan, waxy maize amylopectin, soluble starch and maltose. When the transconjugant cells of E. coli were grown with pullulan or maltose, most pullulanase was produced intracellularly, whereas K. aerogenes produced pullulanase extracellularly. Retransfer of the pulk gene from E. coli to K. aerogenes by conjugation resulted in an increase of the production of extracellular pullulanase. 相似文献
33.
Nobutaka Takahashi Akinori Suzuki Yasuo Kimura Satoshi Miyamoto Saburo Tamura Takashi Mitsui 《Bioscience, biotechnology, and biochemistry》2013,77(9):1115-1122
Piericidin B was isolated from mycellia of Streptomyces mobaraensis besides piericidin A. On the basis of IR, NMR and mass spectral studies together with chemical evidences, its structure was assigned as Id. Its physiological activities are also deescribd. 相似文献
34.
Manh Huynh Vu Tetsu Ando Shigeo Yoshida Nobutaka Takahashi Sadahiro Tatsuki Kazumasa Katagiri 《Bioscience, biotechnology, and biochemistry》2013,77(7):1615-1617
Ferredoxin-nitrite reductase (EC 1.7.7.1), an enzyme which catalyzes the 6-electron reduction of nitrite to ammonia, has been isolated from Spinacia oleracea. The isolated enzyme was homogeneous by disc electrophoresis with polyacrylamide gel. The molecular weight of the enzyme was estimated to be 86,000 by Ultrogel AcA 34 gel filtration. In the oxidized form, the enzyme had absorption maxima at 278, 388 (Soret band), 573 (α band) and 690 nm, indicating that siroheme is directly involved in the catalysis of nitrite reduction. This absorption spectrum was modified by sulfite, hydroxylamine and cyanide. The enzyme exhibited electron paramagnetic resonance signals with g values of 6.9 and 5.2, which are characteristic of a high spin Fe3+ -siroheme in the molecule. These signals disappeared upon the addition of dithionite or nitrite. This isolated enzyme also contained four moles of labile sulfide and 7 g-atoms of iron per 86,000 g of protein. 相似文献
35.
Several 4-thiazolone derivatives were synthesized by condensation of appropriate aryl cyanide and ethyl thioglycolate (or derivative), and factors affecting to the reaction rate are discussed. Keto-enol tautomerization of the 4-thiazolones is briefly discussed. 相似文献
36.
Noboru Murofushi Nobutaka Takahashi Takao Yokota Saburo Tamura 《Bioscience, biotechnology, and biochemistry》2013,77(10):1239-1245
A new gibberellin, gibberellin A20 (GA20), was isolated from immature seeds of morning-glory (Pharbitis nil). Its structure was established as 4aα, 7α-dihydroxy-1β-methyl-8-methylenegibbane-1α, 10β-dicarboxylic acid-1→4a lactone (I) on the basis of its physicochemical analysis as well as chemical evidences. GA20 shows marked growth promoting activities on dwarf maize d2 and d5 but weak activities on d1, rice seedling and dwarf pea. 相似文献
37.
Yukiko Kawanabe Hisakazu Yamane Nobutaka Takahashi Teruko Nakamura 《Bioscience, biotechnology, and biochemistry》2013,77(8):2447-2450
GA3 was identified as a major GA in Neurospora crassa by gas chromatography/selected ion monitoring (GC/SIM) and its content was measured at various stages (0~96 hr after inoculation) of conidial germination and mycelial growth. The GA3 content in the fungus was 190ng/g dry weight at the initial stage and then decreased rapidly; that per liter culture decreased soon after the inoculation, and then increased to 17.6 ng 96 hr after inoculation. The GA3 concentration in the culture medium was around 10?11 m throughout the 96-hr incubation. The physiological role of endogenous GA in Neurospora crassa is also discussed. 相似文献
38.
39.
Noboru Murofushi Yoshio Shigematsu Shigehiro Nagura Nobutaka Takahashi 《Bioscience, biotechnology, and biochemistry》2013,77(9):2305-2311
Steviol (ent-13-hydroxykaur-16-en-19-oic acid)* is metabolized by Gibberella fujikuroi in the presence of inhibitors of gibberellin biosynthesis, such as quaternary ammonium salt-type growth retardants, to afford 7β-Miydroxy- and 6β,7β-dihydroxysteviol, gibberelhns A1, A18, A19, A53 and 7β,13-dihydroxykaurenolide. Steviol acetate (ent-13-acetoxykaur-16-en-19-oic acid) is also metabolized to the 6β,7β-dihydroxy-derivative and to the 13-acetyl derivatives of gibberellins A17 and A20 and steviol methyl ester (methyl ent-13-hydroxykaur-16-en-19-oate) into the monohydroxy-, dihydroxy- and hydroxyoxo-derivatives. These results indicate a low substrate specificity of the enzymes in the fungus and provide a useful preparative methodology of several important plant gibberellins carrying the 13-hydroxyl group. 相似文献
40.
Tetsu Ando Osamu Saito Koshi Arai Nobutaka Takahashi 《Bioscience, biotechnology, and biochemistry》2013,77(11):2643-2649
The female sex pheromone of the oriental corn borer, Ostrinia furnacalis Guenée, was presumed to be composed of (Z)-12-tetradecenyl acetate and its geometrical isomer using electroantennogram technique. From the extracts of female moths, the presence of these compounds in a ratio of ca. 3:2 was confirmed by gas-liquid chromatography and gas-liquid chromatography combined with mass spectrometry in selected ion monitoring mode. Since the male moths were not attracted to mixtures of the two synthetic compounds, the presence of minor component(s) was suggested. 相似文献