Cross microbial antigens of the human bronchopulmonary apparatus]

The work was undertaken to assess the antigenic affinity of some Neisseria perflava, Klebsiella pneumoniae, Staphylococcus aureus strains serving as active sensitinogen of the human broncho-pulmonary apparatus. With the aid of the complement fixation test, precipitation after Ouchterolony, and immunoelectrophoresis there was established the presence of a series of similar antigenic determinants common for all the three microbes and for determinant responsible for the cross reactions of two microbes only.     

Etiologic role of bacteria of the genus Providencia in acute intestinal diseases

A study was made of morphological, cultural, biochemical properties and antigenic structure (by O-antigen) of 59 strains isolated in group acute intestinal affection of children at the pioneer camp. By the combination of biochemical properties (31 tests) all the cultures isolated were referred to Providencia alcalifaciens of biotype 7. Serological typing with the aid of experimental diagnostic agglutinating O-sera showed these strains to be referred to serological group O2. Identity of the O-antigen of the isolated cultures to the standard O2 strain was confirmed by the results of cross reactions of agglutinin absorption. The results of bacteriological and clinico-epidemiological studies offered a possibility of regarding the isolated Providencia alcalifaciens of serological group O2 as the causative agents of the group intestinal disease in children.     

pH-induced conformational changes of DNA

The electrophoretic mobility of calf thymus DNA has been measured in aqueous buffered solutions as a function of pH. In the pH range 3.7–3.0, two electrophoretic species appear. The faster one migrates with the mobility of native DNA, the slower one migrates with a mobility close to that of thermally denatured DNA. The ratio of the two species varies with pH. Decreasing the pH increase the relative amount of the slower-moving component. These results may be interpreted by assuming that the DNA used in these experiments has a broad heterogeneity of base composition and that the conformational stability with respect to pH increases with increasing G + C content.     

The effect of valinomycin and nigericin on the efficacy of bacteriophage infection of staphylococcal cells

The effect of ionophore antibiotics, valinomycin and nigericin, on the generation of the membrane potential, the pH gradient and the efficacy of phage infection in tetracycline-resistant staphylococci has been studied. Valinomycin at a concentration of 0.5 microM induces the dissipation of the membrane potential, and nigericin at a concentration of 12.0 microM decreases the value of the pH gradient on the membrane of staphylococci. The separate use of antibiotics has no essential influence on the efficacy of phage infection. The combined use of valinomycin and nigerimycin produces the maximum inhibition of phage infection (64.5%) at the stage of the introduction of DNA into the bacterial cell, which is indicative of a definite role played by the membrane potential and the pH gradient in the transport of phage DNA into staphylococcal cells.