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131.
Vanessa Paes da Cruz Maria Ligia Oliveira Nobile Fabilene Gomes Paim Aisni Mayumi Correia de Lima Adachi Giovana da Silva Ribeiro Daniela Carvalho Ferreira Jos Carlos Pansonato-Alves Patrícia Charvet Claudio Oliveira Fausto Foresti 《Genetics and molecular biology》2021,44(2)
The chromosomes of two freshwater stingrays, Potamotrygon motoro and Potamotrygon sp., from the Amazon River basin in Brazil were investigated using integrated molecular (cytochrome c oxidase subunit 1) and cytogenetic analyses. Potamotrygon motoro presented intraspecific variation in the diploid number, with 2n=66 in the females and 2n=65 in the males, while Potamotrygon sp. had a karyotype with 66 chromosomes, in both sexes. The C-banding revealed the presence of heterochromatic blocks accumulated in the centromeric region of all the chromosomes in both species. The FISH assays with 18S DNA probes highlighted the terminal region of three or four chromosome pairs in P. motoro and seven chromosomes in Potamotrygon sp. The rDNA 5S sequences were found in only one chromosomal pair in both species. The interspecific genetic distance based on the COI sequences, between P. motoro and Potamotrygon sp. from Amazon River was 10.8%, while that between the Amazonian P. motoro and Potamotrygon amandae from the Paraná River was 2.2%, and the genetic distance between Potamotrygon sp. and P. amandae was 11.8%. In addition to the new insights on the cytogenetics of the study species, the results of the present study confirmed the existence of heteromorphic sex-linked chromosomes in P. motoro. 相似文献
132.
Evidence for a role of a vicinal dithiol in the transport of gamma-butyrobetaine in Agrobacterium sp
An Agrobacterium sp. isolated from soil is able to use gamma-butyrobetaine as its sole source of carbon and nitrogen. The involvement of thiol groups for active transport of gamma-butyrobetaine was investigated by use of the thiol alkylating reagent N-ethylmaleimide (NEM) and the dithiol specific reagent phenylarsine oxide (PAO). Both reagents strongly inhibited gamma-butyrobetaine uptake, but also induced the release of the accumulated substrate, suggesting that the transport system either contains a dithiol-dependent protein or that a small thiol-containing molecule is implicated in the uptake phenomenon. 相似文献
133.
A Nobile M Nobile G Germana' V Cavallari G Passantino 《Bollettino della Società italiana di biologia sperimentale》1979,55(16):1552-1558
The Authors describe the histologic report of the eye and the first part of optic nerve of young goats and rabbits exposed to high grade barometric depression (850/cm2) limited to cranium. 相似文献
134.