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31.
Increased insulin sensitivity and reduced adiposity in phosphatidylinositol 5-phosphate 4-kinase beta-/- mice
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Lamia KA Peroni OD Kim YB Rameh LE Kahn BB Cantley LC 《Molecular and cellular biology》2004,24(11):5080-5087
Phosphorylated derivatives of the lipid phosphatidylinositol are known to play critical roles in insulin response. Phosphatidylinositol 5-phosphate 4-kinases convert phosphatidylinositol 5-phosphate to phosphatidylinositol 4,5-bis-phosphate. To understand the physiological role of these kinases, we generated mice that do not express phosphatidylinositol 5-phosphate 4-kinase beta. These mice are hypersensitive to insulin and have reduced body weights compared to wild-type littermates. While adult male mice lacking phosphatidylinositol 5-phosphate 4-kinase beta have significantly less body fat than wild-type littermates, female mice lacking phosphatidylinositol 5-phosphate 4-kinase beta have increased insulin sensitivity in the presence of normal adiposity. Furthermore, in vivo insulin-induced activation of the protein kinase Akt is enhanced in skeletal muscle and liver from mice lacking phosphatidylinositol 5-phosphate 4-kinase beta. These results indicate that phosphatidylinositol 5-phosphate 4-kinase beta plays a role in determining insulin sensitivity and adiposity in vivo and suggest that inhibitors of this enzyme may be useful in the treatment of type 2 diabetes. 相似文献
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Nicolas Collongues Maria C. Alcaro Frédéric Blanc Francesca Barbetti Marie Fleury Elisa Peroni Paolo Rovero Gabrielle Rudolf Francesco Lolli Élisabeth Trifilieff Anna‐Maria Papini Jérôme de Seze 《Journal of peptide science》2013,19(1):25-32
The detection of reactivity against autoantigens plays a crucial role in the diagnosis of autoimmune diseases. However, only a few autoantibodies are known in each disease, and their precise targets are often not precisely defined. In neuromyelitis optica (NMO), an autoimmune disease of the central nervous system, anti‐aquaporin 4 antibodies are currently the only available immunological markers, although they are not detected in 10–50% of patients. Using enzyme‐linked immunosorbent assays, we evaluated the reactivity against 19 structurally defined peptides in 26 NMO sera compared with 21 healthy subjects. We observed increased levels of IgG against myelin basic protein sequence MBP(156–175), pyruvate dehydrogenase sequence PDH(167–186) and CSF114(Glc), the last of these having a possible correlation with onset of inflammatory relapse. These preliminary results may suggest that the aquaporin 4 is not the unique target in NMO and that the study of reactivity against these peptides would be helpful for the diagnosis and follow‐up of the disease. Complementary studies are however warranted to confirm these results. Copyright © 2012 European Peptide Society and John Wiley & Sons, Ltd. 相似文献
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Tagliari Mario M. Vieilledent Ghislain Alves Jonatas Silveira Thiago C. L. Peroni Nivaldo 《Biodiversity and Conservation》2021,30(12):3665-3684
Biodiversity and Conservation - One of Brazil’s most threatened tropical biome is the Atlantic Forest. This biome has distinct forest formations, as the Araucaria Mixed Forest, a sub-tropical... 相似文献
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George Shigueki Yasui Laura Satiko Okada Nakaghi Paulo Sérgio Monzani Nivaldo Ferreira do Nascimento Matheus Pereira dos Santos Caio Augusto Gomes Goes Fábio Porto-Foresti José Augusto Senhorini 《Zeitschrift fur angewandte Ichthyologie》2020,36(3):336-341
The streaked prochilod Prochilodus lineatus is an important fish species from the Neotropical region. In this study, a protocol of triploidization was established by temperature shock. Fertilized eggs were shocked at 2 min post-fertilization at 0, 38, 40 and 42°C. At 0°C, the embryos were maintained for 30 min, while the rest for 2 min. Heat shocked embryos and control (during all experiment) were incubated at 27°C. The ploidy status was confirmed by flow cytometry, erythrocyte diameter, conventional cytogenetics (Giemsa staining), chromosome banding and fluorescence in situ hybridization using 5S and 18S rDNA probes. Heat-shock at 40°C produced 96.7% of triploids and such a procedure did not reduce the fertilization, hatching rate and the percentage of normal embryos. The use of chromosome banding and FISH gave rise to effective procedure to identify triploids. The data obtained here is innovative for the species and bring new information for basic and applied studies. 相似文献
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Lippy Faria Marques Nivaldo L. Speziali Flávia Cavalieri Machado 《Inorganica chimica acta》2011,365(1):454-457
The coordination polymer named {[Co(H2O)4(BPP)][Co(dipic)2]·H2O}n (dipic = 2,6-pyridinedicarboxylate and BPP = 1,3-bis(4-pyridyl)propane) has been synthesized by the diffusion method and characterized by thermal analysis, vibrational spectroscopy and single crystal X-ray diffraction analysis. The compound is formed by an one-dimensional polymeric cationic chain, in which the BPP ligands connect the Co2 sites, and an anionic moiety formed by Co1 center and two dipic2− anions. Both crystallographic independent metal sites adopt a distorted octahedral geometry. Co2 center is coordinated by two nitrogen atoms from BPP ligands and four oxygen atoms from aqua ligands, while Co1 center is coordinated by two nitrogen atoms and four oxygen atoms from two dipic2− anions. The cationic and anionic moieties are connected through hydrogen bonding interactions leading to a 3D supramolecular array. 相似文献
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Neto JL Lira CB Giardini MA Khater L Perez AM Peroni LA dos Reis JR Freitas-Junior LH Ramos CH Cano MI 《Biochemical and biophysical research communications》2007,358(2):417-423
Replication protein A (RPA) is a highly conserved heterotrimeric single-stranded DNA-binding protein involved in different events of DNA metabolism. In yeast, subunits 1 (RPA-1) and 2 (RPA-2) work also as telomerase recruiters and, in humans, the complex unfolds G-quartet structures formed by the 3' G-rich telomeric strand. In most eukaryotes, RPA-1 and RPA-2 bind DNA using multiple OB fold domains. In trypanosomatids, including Leishmania, RPA-1 has a canonical OB fold and a truncated RFA-1 structural domain. In Leishmania amazonensis, RPA-1 alone can form a complex in vitro with the telomeric G-rich strand. In this work, we show that LaRPA-1 is a nuclear protein that associates in vivo with Leishmania telomeres. We mapped the boundaries of the OB fold DNA-binding domain using deletion mutants. Since Leishmania and other trypanosomatids lack homologues of known telomere end binding proteins, our results raise questions about the function of RPA-1 in parasite telomeres. 相似文献
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Gabriel Silva dos Santos Jamerson Aguiar‐Santos Antnio Carlos Leal de Castro Nivaldo Magalhes Piorski 《Zeitschrift fur angewandte Ichthyologie》2019,35(5):1169-1171
Parameters of the length‐weight relationship (LWR) were estimated for seven fish species from Amazonian Equatorial coast of Maranhão, Brazil. Samplings were carried out in three sample points of the lower stretch of the Itapecuru River (2°57'6.2"S and 44°14'26.5"W; 3°0'33.0"S and 44°15'54.7"W; 3°3'42.9"S and 44°15'1"W). The specimens were caught quarterly from June 2012 to August 2014 using monofilament gillnets (10, 20, 30, 40, 50, 60, 70 and 80 mm between knots) from 10 m to 30 m long and 4 m to 6 m high. This study provides the LWR parameters for Schizodon dissimilis, Curimata macrops, Prochilodus lacustris, Geophagus surinamensis, Hassar affinis, Platydoras brachylecis and Hypostomus plecostomus. 相似文献
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Laura Zemany Bettina J. Kraus Julie Norseen Tsugumichi Saito Odile D. Peroni Randy L. Johnson Barbara B. Kahn 《Molecular and cellular biology》2014,34(6):1170-1186
To investigate the mechanisms by which elevated retinol-binding protein 4 (RBP4) causes insulin resistance, we studied the role of the high-affinity receptor for RBP4, STRA6 (stimulated by retinoic acid), in insulin resistance and obesity. In high-fat-diet-fed and ob/ob mice, STRA6 expression was decreased 70 to 95% in perigonadal adipocytes and both perigonadal and subcutaneous adipose stromovascular cells. To determine whether downregulation of STRA6 in adipocytes contributes to insulin resistance, we generated adipose-Stra6−/− mice. Adipose-Stra6−/− mice fed chow had decreased body weight, fat mass, leptin levels, insulin levels, and adipocyte number and increased expression of brown fat-selective markers in white adipose tissue. When fed a high-fat diet, these mice had a mild improvement in insulin sensitivity at an age when adiposity was unchanged. STRA6 has been implicated in retinol uptake, but retinol uptake and the expression of retinoid homeostatic genes (encoding retinoic acid receptor β [RARβ], CYP26A1, and lecithin retinol acyltransferase) were not altered in adipocytes from adipose-Stra6−/− mice, indicating that retinoid homeostasis was maintained with STRA6 knockdown. Thus, STRA6 reduction in adipocytes in adipose-Stra6−/− mice fed chow resulted in leanness, which may contribute to their increased insulin sensitivity. However, in wild-type mice with high-fat-diet-induced obesity and in ob/ob mice, the marked downregulation of STRA6 in adipocytes and adipose stromovascular cells does not compensate for obesity-associated insulin resistance. 相似文献
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Sigala S Peroni A Mirabella G Fornari S Palazzolo F Pezzotti G Simeone C Cunico SC Spano P 《Life sciences》2004,76(4):417-427
A detailed study of the presence of alpha1 AR binding sites and alpha1 AR subtype mRNA expression in human urinary bladder areas involved in the micturition (i.e. detrusor, trigone and neck) is reported here, investigating whether or not there are differences between sexes. Results obtained indicated that alpha1 AR proteins were detectable in each bladder area. In both sexes, the detrusor and the neck expressed similar levels of alpha1 ARs: respectively, detrusor: 14.6 +/- 1.2 in men and 13.1 +/- 1.1 fmol/mg prot in women; neck: 16.9 +/- 3.2 in men and 17.5 +/- 4.1 fmol/mg prot in women. In the trigone, significantly higher alpha1ARs were found in women compared to men (20.6 +/- 1.1 vs 11.7 +/- 0.7 fmol/mg prot). Subtype analysis indicated that in women, each area was endowed with mRNA encoding for each alpha1 AR subtype. The men detrusor expressed alpha1a and alpha1d ARs, while in the trigone and the neck, each subtype was present. Since the detrusor muscle hypertrophy is a marker of bladder obstructive outlet, the selective alpha1 AR subtype targeting arouses much interest, as evidence indicates that there are differences in signalling pathways among the subtypes. Furthermore, the significance of the alpha1 ARs coexpression is still unknown; interestingly, recent papers demonstrate that alpha1 AR subtypes could dimerize. Thus, in the human urinary bladder it may be suggested a potential level of alpha1 AR complexity that could have an impact on drug development. 相似文献