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11.
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内皮素对失血性休克鼠脑微循环的影响及尼莫地平、川芎嗪的治疗作用 总被引:1,自引:1,他引:0
利用大鼠颅骨开窗观察软脑膜微循环的方法研究了内皮素(ET-1)10-10-10-7mol/L对软脑膜微循环的影响以及失血性休克时软脑膜对ET-1的反应性。并用10-7mol/L造成失血性休克后脑血管痉挛的模型,观察尼莫地平、川芎嗪、654-2对内皮素引起血管痉挛的治疗作用。10-9、10-8和10-7mol/L3种浓度ET-1可使软脑膜小动脉、细动脉强烈收缩,收缩率分别为27.7%、46.8%、78.5%,其收缩强度与ET-1的浓度有关。对静脉的作用不明显。10-10mol/LET-1可使细动脉轻度扩张。出血性休克时,软脑膜血流明显减慢,小动脉、细动脉管径对ET-1的收缩作用更敏感,脑组织血流明显减少。尼莫地平具有较好的拮抗ET-1引起软脑膜动脉的收缩和改善局部微循环的作用。川芎嗪也能拮抗ET-1引起软脑膜动脉的收缩,但作用较尼莫地平弱。654-2不能缓解ET-1对软脑膜动脉的收缩作用。 相似文献
13.
慢性缺氧对大鼠膈肌组织化学和超微结构影响的研究 总被引:6,自引:0,他引:6
研究慢性缺氧和参麦注射液治疗对大鼠膈肌组织化学和超微结构的影响,结果显示:缺氧使膈肌SDH活性降低,Ⅰ类纤维极显著减少,运动终板ChE活性极显著降低,线粒体肿胀变性,神经肌肉运动终板突触前部囊泡减少,突触间隙模糊,终板模电子密度降低;参麦治疗可恢复缺氧膈肌的SDH活性及Ⅰ类纤维数目,显著提高终板ChE活性,线粒体结构恢复正常,运动终板突触前部囊泡丰富,突触间隙清晰,终板膜电子密度正常。表明慢性缺氧降低膈肌有氧氧化能力,影响其能量代谢及神经冲动的传递,从而减弱其收缩力,而参安治疗可改善缺氧造成的上述损害,为其临床应用提供了实验医学依据。 相似文献
14.
Pengfei Zhang Yuqin Zhang Qifeng Zhao Tiequan Niu Pengfei Wen Jinjun Liang 《Phyton》2023,92(4):1125-1138
Grape pistil has an important influence on fruit size and quality. However, there were few studies on grape ovary, and the development process of the ovary is still unclear. Therefore, in this paper, four different grape varieties with different lengths of small inflorescences, namely ‘Musct Hambourg’ grape (Vitis vinifera), ‘Concord’ grape (Vitis labrusca), ‘ShanPuTao’ grape (Vitis amurensis) and ‘GongNiang2Hao’ grape (Vitis amurensis × Vitis vinifera) were used as test materials. Four varieties ovary were significant differences by means of stereomicroscope, paraffin section. The expression of ovary determining gene VvAGAMOUS (VvAG) and its development related genes VvCRABS CLAW (VvCRC) andVvAGAMOUS-LIKE 11 (VvAGL11) with similar functions during the development of different grape varieties were preliminarily explored using fluorescence quantitative test. The relationship between VvAG and VvCRC, VvAG and VvAGL11 were analyzed using Y1H assay. Our results showed that there were obvious abdominal sutures on the surface of expect for ‘Musct Hambourg’ grape, and existing poly carpels. The ovary development of ‘ShanPuTao’ and ‘GongNiang2Hao’ grape was completed when the inflorescence length was less than 1 cm, while the ‘Concord’ and ‘Musct Hambourg’ grape were fully developed when the length of inflorescence was 3–4 and 4–5 cm, respectively. VvAG and VvCRC began to express in large quantities after the formation of stamen primordia, while VvAGL11 during the forming of ovule primordia. Therefore, VvAG and VvCRC mainly regulated the development of stamens and carpels and also promote the development of ovules, while VvAGL11 major regulated the development of ovules. The promoters of VvCRC and VvAGL11 were bound by VvAG. This study provides an important theoretical basis for further research on the molecular mechanism of grape ovary development. 相似文献
15.
Niu Yuan Wang Fang Liu Le Zhang Guoliang Qi Bo Liu Xinhai Zhao Hongliang Huang Zhiwei Fan Song Zhang Linqing 《Journal of Plant Growth Regulation》2023,42(1):154-167
Journal of Plant Growth Regulation - 1,2,4-trichlorobenzene (1,2,4-TCB) pollution in fields has become a potential threat to rice growth, yet little is known about the different response of many... 相似文献
16.
脏器微血管对荧光素钠通透性的实验方法 总被引:4,自引:1,他引:3
大鼠颈动脉注射1%FlNa,荧光显微镜下活体观察肠系膜微血管血流状态及FlNa的渗出情况,并在不同时间点经股动脉采血,测定血浆内FlNa浓度随时间的变化,利用组织匀浆测定不同脏器中FlNa的分布,再辅以冰冻切片进行观察。活体观察发现,FlNa注入体内后,经微血管迅速向周围组织渗出,最后汇集于淋巴管,血浆及组织匀浆FlNa浓度的测定表明,FlNa浓度随时间的变化呈指数衰减,各脏器FlNa的分布极不相同。冰冻切片也显示了同样的分布差别。这些结果表明,我们所建立的方法可直观、定量地反映FlNa在微血管的通透情况。 相似文献
17.
18.
褐稻虱在武育粳2号上不同生育期危害对水稻产量结构的影响 总被引:2,自引:0,他引:2
通过试验测定在武育粳2号上水稻拔节期正处于幼穗形成阶段,受褐稻虱危害实粒数减少,产量下降;孕穗期较耐褐稻虱危害,有明显的补偿作用;孕穗末期至灌浆初期为水稻受害最敏感的时期,危害后使结实率和千粒重均明显下降,水稻显著减产;灌浆后期至乳熟期受褐稻虱危害主要导致干粒重降低,且越接近成熟产量损失越低。 相似文献
19.
球形芽孢杆菌Ts—1毒蛋白的分离纯化 总被引:3,自引:2,他引:1
Bacillus sphaericus strain Ts-1 is highly insecticidal to larvae of the mosquito. It's insecticidal component is toxic proteins. The toxin was extracted from spore-crystal complexes by disruption in a Sonicator Cell Disruptor Model W-220F followed by treatment with 0.05 mol/L NaOH. Fraction recovered from chromatography of the spore-crystal complexes on column of Sephadex G-200 were assayed against mosquito larvae and the toxic fractions from gel chromatography were subjected to SDS-PAGE. The toxic proteins in B. sphaericus Ts-1 spore-crystal complex migrated in position corresponding to 42kD and 43kD. Bioassay of the two purified proteins prepared by PAGE indicated that they were all toxic to mosquito larvae. Toxic protein was further purified by DEAE-cellulose chromatography. The toxic protein with a molecular weight of 42kD was obtained. 相似文献
20.
W. F. Li J. Ji G. Wang H. Y. Wang B. L. Niu T. L. Josine 《Russian Journal of Genetics》2011,47(9):1039-1046
Many natural proteins have been developed into drugs and produced for direct application. Identifying improved hosts to achieve
high-level heterologous protein production is a challenge in the study of heterologous protein expression in recombinant yeast.
In this study, a novel high-throughput assay to screen such overproducing Saccharomyces cerevisiae strains was systematically developed. The protocol designed was based on screening host strain derivatives with increased
superoxide dismutase dependent resistance to oxidative stress. Yeast cells transformed with recombinant plasmid carrying SOD1 gene as a reporter responded exquisitely to oxidative stress induced by elevated concentrations of paraquat. Improved yeast
strains resulting from screening clones subjected to genome shuffling through selective pressure argue for a more effective
screening system compared with traditonal selection. Moreover, this approach can be employed in general biochemical analysis
without utilization of flow cytometry or well plate reader. Therefore, it is expected that the high-throughput assay would
make superior strains producing heterologous proteins. 相似文献