全文获取类型
收费全文 | 786篇 |
免费 | 63篇 |
出版年
2021年 | 12篇 |
2018年 | 12篇 |
2017年 | 8篇 |
2016年 | 8篇 |
2015年 | 14篇 |
2014年 | 30篇 |
2013年 | 37篇 |
2012年 | 38篇 |
2011年 | 30篇 |
2010年 | 23篇 |
2009年 | 27篇 |
2008年 | 25篇 |
2007年 | 32篇 |
2006年 | 32篇 |
2005年 | 20篇 |
2004年 | 25篇 |
2003年 | 23篇 |
2002年 | 27篇 |
2001年 | 23篇 |
2000年 | 18篇 |
1999年 | 25篇 |
1998年 | 6篇 |
1997年 | 6篇 |
1996年 | 6篇 |
1994年 | 8篇 |
1993年 | 7篇 |
1992年 | 15篇 |
1991年 | 16篇 |
1990年 | 19篇 |
1989年 | 14篇 |
1988年 | 17篇 |
1987年 | 29篇 |
1986年 | 13篇 |
1985年 | 16篇 |
1984年 | 10篇 |
1983年 | 18篇 |
1982年 | 7篇 |
1981年 | 9篇 |
1980年 | 7篇 |
1979年 | 14篇 |
1978年 | 9篇 |
1977年 | 9篇 |
1976年 | 9篇 |
1975年 | 10篇 |
1974年 | 7篇 |
1973年 | 15篇 |
1972年 | 6篇 |
1971年 | 6篇 |
1969年 | 5篇 |
1967年 | 6篇 |
排序方式: 共有849条查询结果,搜索用时 318 毫秒
771.
772.
773.
H. Kalamarz‐Kubiak I. Meiri‐Ashkenazi A. Kleszczyńska H. Rosenfeld 《Journal of fish biology》2014,84(2):448-458
This study aimed at determining whether in vitro secretion of two neuropeptides, arginine vasotocin (AVT) and isotocin (IT), from pituitary cells of gilthead sea bream Sparus aurata was affected by cortisol and urotensin (UI). Pituitary cells were exposed to 1·4 × 10?8, 1·4 × 10?7 and 0·4 × 10?6 M cortisol and 10?12, 10?10 and 10?8 M UI for 6, 24 and 48 h, respectively. AVT and IT contents were determined in the culture media by high‐performance liquid chromatography (HPLC). An increase in AVT secretion and a decrease in IT secretion were observed at all cortisol doses. UI increased AVT secretion after 6 h of incubation at all doses. After 24 h, however, only the highest dose of UI still displayed an effect. IT secretion was not influenced by UI. It was thus demonstrated that cortisol does influence AVT and IT secretion from S. aurata pituitary cells, while UI regulates AVT secretion, as a component of hypothalamic–pituitary–interrenal (HPI) axis in this species. 相似文献
774.
775.
To examine a possible role for IGF-II in the regulation of IGF-I receptors we measured 125I-IGF-I binding on IM-9 cells following pre-incubation with IGF-II/IGF-I mixtures, purified MSA (a rat IGF-II-like peptide), pure IGF-I, or insulin. Whereas all preparations tested induced down-regulation of IGF-I binding after 20 hours, distinct differences were noted after six hour pre-incubation: IGF-I (100 ng/ml) and insulin (1 microgram/ml) both induced down-regulation of IGF-I binding (15 +/- 2% and 19 +/- 2% respectively). However, a mixture of IGF-II and IGF-I (100 ng/ml each) induced consistent up-regulation of IGF-I binding (16 +/- 2%) (mean +/- SE, n = 14), and a preparation enriched in IGF-II (250 ng/ml IGF-II and 75 ng/ml IGF-I) induced 20 +/- 5% (n = 3) up-regulation at six hours. Purified MSA (200 ng/ml) induced 15% up-regulation of IGF-I binding at six hours. Scatchard analysis of displacement curves showed that increased binding was due to loss of low affinity binding, with enhancement of high affinity sites. The up-regulation of IGF-I binding was unaffected by treatment with 0.1 mM cycloheximide, but was blunted by 5 microM colchicine. It is concluded that 1. IGF-II induces up-regulation of IGF-I receptors on IM-9 cells following 6 hour pre-incubation; 2. This phenomenon is not mimicked by the structurally-related peptides IGF-I or insulin; The up-regulation is due to enhanced high affinity binding sites.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
776.
J L Pico C Choquet C Rosenfeld A Sharif R Bourillon 《Differentiation; research in biological diversity》1976,5(2-3):115-117
Lectin binding on the cell surface was measured by the method of Kornfeld [16] using three tritiated lectins: Robinia pseudo acacia, Concanavalin A and Ricinus. It has been shown that the number of binding sites for Robinia and Con A decreases after the establishment of a leukaemic cell line, whereas the affinity constant increases for Robinia. The relationship between lectin binding and cell growth was carried out on 5 cell lines, 3 of leukaemic and 2 of normal origin. In all cases the maximum number of sites was determined at the time of doubling for the cell population; the affinity constant reciprocally decreases at this moment. For Robinia, a difference was found in both cell growth and the number of sites between an Epstein-Barr virus (EBV) negative leukaemic cell line and the other EBV positive cell lines. Lectins offer a quantitative method for measuring membrane structure variations during and after establishment of cell lines. 相似文献
777.
778.
779.
The simultaneous extraction and esterification of prostaglandins in aqueous solution is described. High yield of esters can be achieved in a one minute reaction time. The specificity of the alkylation can be controlled by the counterion. Thus, a long chain carboxylic acid can be selectively extracted and derivatized in the presence of PGF2α. 相似文献
780.