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81.
Jain S Mythily S Ahmed RS Arora VK Banerjee BD 《Indian journal of biochemistry & biophysics》2010,47(6):388-392
The effect of triazophos (O, O-diethyl O-1-phenyl-1 H-1, 2, 4-triazol-3-yl phosphorothioate), a widely used insecticide was studied on the induction of oxidative stress and histological alterations at sub-chronic doses in male albino rats. Oral administration of triazophos at concentrations of 1.64, 3.2 and 8.2 mg/kg body wt for 30 days produced dose as well as time-dependent increase in the lipid peroxidation (determined by malondialdehyde levels) and glutathione-S-transferase (GST) activity in serum with aconcomitant decrease in ferric reducing ability of plasma (FRAP) and blood glutathione (GSH) content. Histopathological examination of liver of triazophos-treated rats showed significant and progressive degenerative changes as compared to control, which could be due to induction of oxidative stress. However, no significant histopathological changes were observed in spleen, kidney and brain at either dose of triazophos with respect to control. These results indicated that oral administration of triazophos was associated with enhanced lipid peroxidation and compromised antioxidant defence in rats in dose and time-dependent manner. Thus the present study demonstrated for the first time the role of oxidative stress as the important mechanism involved in the stimulation of hepatic histoarchitectural alterations at sub-chronic doses of triazophos in rats. 相似文献
82.
Ashwini V. Rajwade Ritu S. Arora Narendra Y. Kadoo Abhay M. Harsulkar Prakash B. Ghorpade Vidya S. Gupta 《Molecular biotechnology》2010,45(2):161-170
The objective of this study was to analyze the genetic relationships, using PCR-based ISSR markers, among 70 Indian flax (Linum
usitatissimum L.) genotypes actively utilized in flax breeding programs. Twelve ISSR primers were used for the analysis yielding 136 loci,
of which 87 were polymorphic. The average number of amplified loci and the average number of polymorphic loci per primer were
11.3 and 7.25, respectively, while the percent loci polymorphism ranged from 11.1 to 81.8 with an average of 63.9 across all
the genotypes. The range of polymorphism information content scores was 0.03–0.49, with an average of 0.18. A dendrogram was
generated based on the similarity matrix by the Unweighted Pair Group Method with Arithmetic Mean (UPGMA), wherein the flax
genotypes were grouped in five clusters. The Jaccard’s similarity coefficient among the genotypes ranged from 0.60 to 0.97.
When the omega-3 alpha linolenic acid (ALA) contents of the individual genotypes were correlated with the clusters in the
dendrogram, the high ALA containing genotypes were grouped in two clusters. This study identified SLS 50, Ayogi, and Sheetal
to be the most diverse genotypes and suggested their use in breeding programs and for developing mapping populations. 相似文献
83.
N. K. Arora Ekta Khare S. Singh D. K. Maheshwari 《World journal of microbiology & biotechnology》2010,26(5):811-816
Most of the legume crops are affected by metal stress present in the soil mainly due to contaminated agrochemicals and sewage
sludge. The effect of aluminium, and heavy metals copper, iron and molybdenum on growth and activity of enzymes of fast and
slow growing rhizobial sps. was studied. Sinorhizobium meliloti RMP5 was found to be more tolerant to metal stress than Bradyrhizobium BMP1. Both the strains were extremely sensitive to Al than other metals. Al was much more deleterious for the enzymatic activities
(nitrate reduction, nitrite reduction, nitrogenase and uptake hydrogenase) of strain RMP5 and BMP1. Cu showed inhibitory effect on growth and enzyme activities of Bradyrhizobium strain at all concentrations. However, in S. meliloti RMP5 all the tested enzymatic activities increased up to the concentration of 0.1 mM Cu. Fe enhanced the growth and enzyme activities
of S. meliloti RMP5 and Bradyrhizobium BMP1 up to 100 mM concentration. Mo enhanced all the tested enzymatic activities of S. meliloti RMP5 up to 1 mM. Nitrate and nitrite reduction activities of Bradyrhizobium BMP1 increased up to 1 mM concentration. However, nitrogenase and hydrogenase activities of Bradyrhizobium BMP1 got enhanced only up to 0.5 mM Mo. Both Fe and Mo are the key components of the enzyme nitrogenase and nitrate reductase
and enhanced the growth and enzyme activities of both the sps. The study of physiology of nitrogen fixing ability of both
fast and slow growing rhizobial strains reported that the supplementation of Mo and Fe in soils along with the biological
formulations will enhance the process of symbiotic nitrogen fixation. 相似文献
84.
85.
Bandamaravuri Kishore Babu S. S. Reddy Mukesh K. Yadav M. Sukumar Vijendra Mishra A. K. Saxena Dilip K. Arora 《Indian journal of microbiology》2010,50(2):199-204
Genetic diversity analysis of Macrophomina phaseolina isolates obtained from different host range and diverse geographical locations in India was carried out using RAPD fingerprinting.
Of the thirteen 10-mer random primers used, primer OPB-08 gave the maximum polymorphism and the UPGMA clustering could separate
50 isolates in to ten groups at more than 65% similarity level. The ten clusters correlated well with the geographical locations
with exceptions for isolates obtained from Eastern and Western Ghats. There was a segregation of isolates from these two geographical
locations in to two clusters thus, distributing 10 genotypes in to eight geographical locations. All the isolates M. phaseolina irrespective of their host and geographical origin, exhibited two representative monomorphic bands at 250 bp and 1 kb, presence
of these bands suggests that isolates might have evolved from a common ancestor but due to geographical isolation fallowed
by natural selection and genetic drift might have segregated in to subpopulations. Genetic similarity in the pathogenic population
reflects the dispersal of single lineage in all locations in India. 相似文献
86.
R. Arora S. Bhatia B. P. Mishra R. Sharma A. K. Pandey B. Prakash A. Jain 《Biochemical genetics》2010,48(3-4):304-311
The genetic polymorphism of the β-lactoglobulin (β-LG) gene was determined in 638 animals belonging to 15 native Indian sheep breeds reared in different agroecological regions for various production traits. Variants of β-LG were found using PCR–RFLP of genomic DNA. Rsa1 restriction enzyme digestion of a 120-bp PCR fragment of exon 2 of β-LG revealed two genetic variants, A (0.37) and B (0.63), and the three genotypes AA (0.175), AB (0.389), and BB (0.436). The differences in allelic frequency were not significant across the breeds, irrespective of their geographic origin and utility (χ2 test, P > 0.05). The pattern of occurrence of allelic variants revealed that the B allele was more frequent in the majority of the Indian breeds than in breeds reported from countries of Southwest Asia, Eastern and Central Europe, and the Mediterranean. A higher level of heterozygosity (0.422) was discerned, despite the declining status of several of the Indian breeds. These findings revealed that Indian sheep are predominantly of the β-LG B type. 相似文献
87.
Stabilized α-helices and nonpeptidic helix mimetics have emerged as powerful molecular scaffolds for the discovery of protein-protein interaction inhibitors. Protein-protein interactions often involve large contact areas, which are often difficult for small molecules to target with high specificity. The hypothesis behind the design of stabilized helices and helix mimetics is that these medium-sized molecules may pursue their targets with higher specificity because of a larger number of contacts. This protocol describes an optimized synthetic strategy for the preparation of stabilized α-helices that feature a carbon-carbon linkage in place of the characteristic N-terminal main-chain hydrogen bond of canonical helices. Formation of the carbon-carbon bond is enabled by a microwave-assisted ring-closing metathesis reaction between two terminal olefins on the peptide chain. The outlined strategy allows the synthesis and purification of a hydrogen bond surrogate (HBS) α-helix in ~ 1 week. 相似文献
88.
Estimates of (co)variance components and genetic parameters for body weights and first greasy fleece weight in Bharat Merino sheep 总被引:2,自引:0,他引:2
Gowane GR Chopra A Prince LL Paswan C Arora AL 《Animal : an international journal of animal bioscience》2010,4(3):425-431
(Co)variance components and genetic parameters of weight at birth (BWT), weaning (3WT), 6, 9 and 12 months of age (6WT, 9WT and 12WT, respectively) and first greasy fleece weight (GFW) of Bharat Merino sheep, maintained at Central Sheep and Wool Research Institute, Avikanagar, Rajasthan, India, were estimated by restricted maximum likelihood, fitting six animal models with various combinations of direct and maternal effects. Data were collected over a period of 10 years (1998 to 2007). A log-likelihood ratio test was used to select the most appropriate univariate model for each trait, which was subsequently used in bivariate analysis. Heritability estimates for BWT, 3WT, 6WT, 9WT and 12WT and first GFW were 0.05 ± 0.03, 0.04 ± 0.02, 0.00, 0.03 ± 0.03, 0.09 ± 0.05 and 0.05 ± 0.03, respectively. There was no evidence for the maternal genetic effect on the traits under study. Maternal permanent environmental effect contributed 19% for BWT and 6% to 11% from 3WT to 9WT and 11% for first GFW. Maternal permanent environmental effect on the post-3WT was a carryover effect of maternal influences during pre-weaning age. A low rate of genetic progress seems possible in the flock through selection. Direct genetic correlations between body weight traits were positive and ranged from 0.36 between BWT and 6WT to 0.94 between 3WT and 6WT and between 6WT and 12WT. Genetic correlations of 3WT with 6WT, 9WT and 12WT were high and positive (0.94, 0.93 and 0.93, respectively), suggesting that genetic gain in post-3WT will be maintained if selection age is reduced to 3 months. The genetic correlations of GFW with live weights were 0.01, 0.16, 0.18, 0.40 and 0.32 for BWT, 3WT, 6WT, 9WT and 12WT, respectively. Correlations of permanent environmental effects of the dam across different traits were high and positive for all the traits (0.45 to 0.98). 相似文献
89.
Choudhary S Vathulya M Mantri R Arora P Triparty SS 《Plastic and reconstructive surgery》2010,126(6):2297-8; author reply 2298
90.