全文获取类型
收费全文 | 325篇 |
免费 | 10篇 |
出版年
2023年 | 3篇 |
2022年 | 2篇 |
2021年 | 8篇 |
2020年 | 6篇 |
2019年 | 7篇 |
2018年 | 7篇 |
2017年 | 3篇 |
2016年 | 9篇 |
2015年 | 18篇 |
2014年 | 19篇 |
2013年 | 28篇 |
2012年 | 39篇 |
2011年 | 27篇 |
2010年 | 13篇 |
2009年 | 11篇 |
2008年 | 23篇 |
2007年 | 12篇 |
2006年 | 14篇 |
2005年 | 18篇 |
2004年 | 11篇 |
2003年 | 6篇 |
2002年 | 10篇 |
2001年 | 5篇 |
2000年 | 5篇 |
1999年 | 1篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1989年 | 2篇 |
1988年 | 2篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1984年 | 4篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1978年 | 1篇 |
1977年 | 3篇 |
排序方式: 共有335条查询结果,搜索用时 62 毫秒
171.
Kumar Surarapu Lava Singh Ravinder Gurao Ankita Mishra S. K. Kumar Prem Vohra Vikas Niranjan Saket Kumar Sodhi Monika Dash S. K. Sarangdhar S. Mukesh Manishi Kataria Ranjit Singh 《Molecular biology reports》2022,49(7):6029-6040
Molecular Biology Reports - India has a vast riverine and swamp buffalo diversity adapted to various agro-ecological conditions. In the present study, genetic diversity data for 10 different... 相似文献
172.
Haiming Xu Nikolay L. Malinin Niranjan Awasthi Roderich E. Schwarz Margaret A. Schwarz 《The Journal of biological chemistry》2015,290(15):9753-9766
Pro-endothelial monocyte-activating polypeptide II (EMAP II), one component of the multi-aminoacyl tRNA synthetase complex, plays multiple roles in physiological and pathological processes of protein translation, signal transduction, immunity, lung development, and tumor growth. Recent studies have determined that pro-EMAP II has an essential role in maintaining axon integrity in central and peripheral neural systems where deletion of the C terminus of pro-EMAP II has been reported in a consanguineous Israeli Bedouin kindred suffering from Pelizaeus-Merzbacher-like disease. We hypothesized that the N terminus of pro-EMAP II has an important role in the regulation of protein-protein interactions. Using a GFP reporter system, we defined a putative leucine zipper in the N terminus of human pro-EMAP II protein (amino acid residues 1–70) that can form specific strip-like punctate structures. Through GFP punctum analysis, we uncovered that the pro-EMAP II C terminus (amino acids 147–312) can repress GFP punctum formation. Pulldown assays confirmed that the binding between the pro-EMAP II N terminus and its C terminus is mediated by a putative leucine zipper. Furthermore, the pro-EMAP II 1–70 amino acid region was identified as the binding partner of arginyl-tRNA synthetase, a polypeptide of the multi-aminoacyl tRNA synthetase complex. We also determined that the punctate GFP pro-EMAP II 1–70 amino acid aggregate colocalizes and binds to the neurofilament light subunit protein that is associated with pathologic neurofilament network disorganization and degeneration of motor neurons. These findings indicate the structure and binding interaction of pro-EMAP II protein and suggest a role of this protein in pathological neurodegenerative diseases. 相似文献
173.
174.
175.
Gopinath Rana Niranjan K. Mandal Dhruba Sakha Bhim C. Meikap 《Geomicrobiology journal》2013,30(9):846-852
Global warming due to the emission of carbon dioxide (CO2) gas, by the anthropogenic sources is a great threat to the environment. A part of this gas is absorbed by soil bacteria as well as aquatic bacteria and it is converted into insoluble calcium carbonate (CaCO3) or calcite. Increased calcite concentration in water and agricultural land creates many problems to the human. In this investigation, an attempt has been made to carry out some experiments to isolate some bacteria from cow dung, which have both calcite solubilization and urease activities. Isolated bacteria solubilize calcite due to the secretion of citric acid, oxalic acid and sanazine pigment. All bacterial isolates were identified by 16S rRNA gene sequencing and the phylogenetic relationship among them was also studied using MEGA 6 software. 相似文献
176.
Sandeep Goel Ranjeet Singh Mahla Sanjay Kumar Suman Niranjan Reddy Hiroshi Imai 《European Journal of Wildlife Research》2011,57(3):663-667
Spermatogonia are germ cells that initiate spermatogenesis in mammalian testis and they are the only cells in adult body capable
of dividing both mitotically and meiotically. Therefore, isolation and preservation of spermatogonia can provide an alternative
method for preservation of genetic pool of endangered animals. To achieve this objective, it is essential to identify markers
that can specifically distinguish spermatogonia from other cells in the testis. In the present study, anti-ubiquitin C-terminal
hydroxylase-1 (UCHL-1/PGP 9.5) antibody specifically recognized spermatogonia in the testis of wild and domestic bovid. The
size of the UCHL-1 protein in various species of bovid testes was identical, and similar to that in mice. Furthermore, UCHL-1
staining could be utilized for the identification of spermatogonia in isolated testicular cells from wild bovids suggesting
that UCHL-1 protein expression could be used as a specific marker for spermatogonia in bovid family. 相似文献
177.
178.
Rituraj Niranjan 《Molecular neurobiology》2013,48(3):412-428
Significant bodies of evidences have shown different mechanisms known to be the etiological cause of Alzheimer’s disease (AD) involving amyloid-beta protein accumulation, chronic inflammatory reactions, oxidative stress, proteasome inhibition, and high-cholesterol level, but the presize etiology of AD still remains enigmatic. Recent studies indicate that these mechanisms seem to be interlinked, and neuroinflammation emerges as a major regulatory and commen factor in all these mechanisms. In amyloid-beta protein, induced neurodegenerative hypothesis of AD inflammatory cytokines IFN-γ, TNF-α, interleukin (IL)-1α plays an important role in the progression of the disease. In cholesterol induced hypothesis liver X receptor mediated IL-4 also plays a major role in the progression of neuroinflammation. Notably, Omi and HtrA2 proteases play very important functions in neuronal dysfunction, which may lead to neurodegeneration. Further at genetic level, alterations in the genes occur especially in APP, PSEN1, PSEN2, APO E(ε4), ADAM12, and SH3MD1 which mediate neurodegeneration. Additionaly, The role of SP-1, NF-κB, and BCAE-1 is critical in the regulation of neuroinflammation-associated disease pathogenesis. All together, in this review, we discus the importance of neuroinflammatory mediators and their mechanistic role in the process of AD neurodegeneration. 相似文献
179.
Jason Arsenault Sabine A.G. Cuijpers Dhevahi Niranjan Bazbek Davletov 《Journal of cellular biochemistry》2014,115(12):2047-2054
Transfection of DNA has been invaluable for biological sciences, yet the effects upon membrane homeostasis are far from negligible. Here, we demonstrate that Neuro2A cells transfected using Lipofectamine LTX with the fluorescently coupled Botulinum serotype A holoenzyme (EGFP‐LcA) cDNA express this SNAP25 protease that can, once translated, escape the transfected host cytosol and become endocytosed into untransfected cells, without its innate binding and translocation domains. Fluorescent readouts revealed moderate transfection rates (30–50%) while immunoblotting revealed a surprisingly total enzymatic cleavage of SNAP25; the transgenic protein acted beyond the confines of its host cell. Using intracellular dyes, no important cytotoxic effects were observed from reagent treatment alone, which excluded the possibility of membrane ruptures, though noticeably, intracellular acidic organelles were redistributed towards the plasma membrane. This drastic, yet frequently unobserved, change in protein permeability and endosomal trafficking following reagent treatment highlights important concerns for all studies using transient transfection. J. Cell. Biochem. 115: 2047–2054, 2014. © 2014 Wiley Periodicals, Inc. 相似文献
180.
Rocktotpal Konwarh Dipankar Kalita Charulata Mahanta Manabendra Mandal Niranjan Karak 《Applied microbiology and biotechnology》2010,87(6):1983-1992
The present work reports the integration of polymer matrix-supported nanomaterial and enzyme biotechnology for development
of industrially feasible biocatalysts. Aqueous leaf extract of Mesua ferrea L. was used to prepare silver nanoparticles distributed within a narrow size range (1–12 nm). In situ oxidative technique
was used to obtain poly(ethylene glycol)-supported iron oxide nanoparticles (3–5 nm). Sonication-mediated mixing of above
nanoparticles generated the immobilization system comprising of polymer-supported silver–iron oxide nanoparticles (20–30 nm).
A commercially important enzyme, Aspergillus niger amyloglucosidase was coupled onto the immobilization system through sonication. The immobilization enzyme registered a multi-fold
increment in the specific activity (807 U/mg) over the free counterpart (69 U/mg). Considerable initial activity of the immobilized
enzyme was retained even after storing the system at room temperature as well as post-repeated magnetic recycling. Evaluation
of the commendable starch saccharification rate, washing performance synergy with a panel of commercial detergents, and antibacterial
potency strongly forwards the immobilized enzyme as a multi-functional industrially feasible system. 相似文献