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91.
Arya R Aslam S Gupta S Bora RS Vijayakrishnan L Gulati P Naithani S Mukherjee S Dastidar S Bhattacharya A Saini KS 《Biotechnology journal》2008,3(7):938-947
Phosphodiesterase 4B (PDE4B) is an important therapeutic target for asthma and chronic obstructive pulmonary disease. To identify PDE4 subtype-specific compounds using high-throughput assays, full-length recombinant PDE4 proteins are needed in bulk quantity. In the present study, full-length human PDE4B2 was expressed in the cellular slime mould Dictyostelium discoideum (Dd). A cell density of 2 x 10(7) cells/mL was obtained and up to 1 mg/L recombinant PDE4B2 was purified through Ni-NTA affinity chromatography. The expressed protein was soluble and its activity was comparable to PDE4B2 protein expressed in mammalian cells (K(m)=1.7 microM). The functional significance of the Dd expression system is supported by the demonstration that, in concert with proteins expressed in mammalian systems, there are no major changes in the affinity for PDE4B2 inhibitors and substrates. These findings thus provide the first evidence that Dd can be utilized for the expression and purification of functionally active full-length human PDE4B2 in large amounts required for high-throughput screening of pharmacologically active compounds against this therapeutic target. 相似文献
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Staphylococcus sp. strain S3/C desulfurized dibenzothiophene/n-hexadecane (3 mg ml–1) in a hydrocarbon aqueous biphasic culture. The resting cells decreased the sulfur content of the hydrocarbon phase by 57% at 2.2 mg l–1 h–1 in the absence of any additional carbon and sulfur source. 相似文献
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Two spontaneous triploid plants of Trigonella corniculata (3n=24) were observed in a population raised from irradiated seeds. Up to seven trivalents per cell were observed. One of the plants appeared to be a double translocation heterozygote involving two non-homologous chromosomes, and therefore could form a quadrivalent, a ring trivalent and bivalents without corresponding univalents. 相似文献
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Immobilization of whole cells in a membraneous form 总被引:3,自引:0,他引:3
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The study investigated the in vitro, ex vivo and in vivo efficacy of ajoene and ciprofloxacin (CIP) alone and in combination against Pseudomonas aeruginosa biofilms and biofilm-associated murine acute pyelonephritis. The ajoene–CIP combination exhibited significant greater (p < 0.05) antimotility and biofilm inhibitory effects than those obtained when they were applied individually. The combined action of the agents resulted in a significant increase in serum sensitivity and phagocytic uptake and killing of P. aeruginosa (p < 0.001) compared to the untreated control. Mice groups treated with an ajoene (25 mg kg?1) and CIP (30 mg kg?1 or 15 mg kg?1) combination showed a significantly (p < 0.001) reduced bacterial load in the kidney and bladder as compared to that of infected controls and mice treated with solo agents on the fifth day post-infection. The decreased levels of biomarkers and photomicrographs of the kidney tissue of the treated mice showed a reduced severity of damage. Hence, the study highlights the antivirulent and therapeutic efficacy of the ajoene-CIP combination at the minimal dosage of CIP. 相似文献