硫酸盐还原一甲烷化两相厌氧消化系统运行工艺条件的研究

以合成废水为基质,研究了采用硫酸盐还原-甲烷化两相厌氧新型工艺处理含高浓度硫酸盐有机废水的系统运行工艺条件.结果表明,酸化-硫酸盐还原反应器的适宜pH为6.5-7.0;500mg/l的S~(2-)使SRB的硫酸盐还原活性下降;208mg/l的[H_2S]_L抑制MPB活性的95.4%;推导出估算气提塔出水回流比R的模型;以得到的工艺条件为依据处理了含19200mg/1的SO_4~(2-)和29400mg/l COD的味精废水.     

毛乌素沙地草地种植管理咨询系统的开发

 本研究初步开发了一个用于毛乌素沙地草地种植管理咨询的专家系统ASSG。这个系统将当地生态系统管理专家们的经验知识和对土壤水分动态的理论研究成果结合起来以产生对于土地利用和种植的作物种类及品种的最优管理策略和最适的植被覆盖率。本系统利用3个层次的推理过程和结构化的知识库,通过咨询过程—步步地引导用户得到最优种植管理策略。在每—层次的推理中,本系统提供给用户多重选择,根据用户的意愿进行下一步的推理。这一推理的交互作用机制为用户获得适宜的咨询结果提供了最大的便利性。本系统的主体部分山Turbo Prolog语言完成,该语言是一种用于开发专家系统的描述性语言。基于土壤水分平衡原理的最优植被覆盖率模型由C语言实现,并应用多语言编程技术将之嵌入程序主体中。系统的样本运行结果表明本系统运行便利,并能产生合理的种植管理策略。     

Fertile Transgenic Indica Rice Produced by Expression of Maize Ubiquitin Promoter-bar Chimaeric Gene in the Protoplasts

We report production of fertile transgenic Indica rice plants by transferring a chimaeric construct consisting of promoter, first exon and intron of maize ubiquitin gene (Ubi-1) and the coding sequences of the bar gene from Streptomyces hygroscopicus to the rice protoplasts through electroporation. In total, 11 plants were regenerated. All of them were fertile and set seeds on maturity. These plants were resistant to high concentration of PPT (400 mg l^?1) which was otherwise toxic to the untransformed controls. The gene was inherited to the progenies of the five plants in Mendelian ratio.     

Axial loading induces rotation of the proximal carpal row bones around unique screw-displacement axes

The changes in carpal bone alignment secondary to the aplication of an axial compressive load through the major wrist motor tendons while the wrist is kept in neutral position (isometric loading) have been investigated on 13 fresh cadaver specimens using a biplanar radiographic method of kinematic analysis. The scaphoid, lunate and triquetrum rotate an average of 5.1, 4.2 and 3.8°, respectively, around different ‘screw displacement axes’, all implying flexion, radial deviation and supination. Based on these findings, a new interpretation of the mechanism by which the wrist remains stable under physiologic loads is provided.     

Identification of the Sites for Suppressor Mutations on the Hemagglutinin Molecule to Temperature-Sensitive Phenotype of the Influenza Virus

A temperature-sensitive (ts) mutant of the influenza virus A/WSN/ 33 strain, ts-134, possessed a defect in intracellular transport at the nonpermissive temperature and marked thermolability of hemagglutinin (HA) activity at 51 C. These were caused by a change at amino acid residue 157 from tyrosine to histidine in the HA protein. We isolated 37 spontaneous revertant clones from ts-134 at the nonpermissive temperature and determined their HA sequences. The deduced amino acid sequences demonstrated that one was a true revertant and the others were revertants with suppressor mutations, each of which had an additional amino acid change besides those of ts-134. The changed amino acids were located at 14 positions on the HA molecule, and eight of them were found in multiple revertants. These were located in five to six distinct regions on the three-dimensional structure of the HA molecule. However, the heat stability of HAs in the revertants was recovered differently depending on the sites of the changed amino acids. The kinetics of transport of the HA protein in the revertants were slightly delayed compared to the wild-type both at permissive and nonpermissive temperatures.     

囊萼紫草属与滇紫草属花粉形态比较研究

本文借助光学显微镜和扫描电镜研究了囊萼紫草属3种和滇紫草属12种植物的花粉形态。囊萼紫草属的花粉为哑铃形或茧形,中等大小,P／E比为1．6一1．67,三孔沟,内孔横长;具小刺状纹饰。滇紫草属的花粉为近长球形或近卵球形,P／E为l—1．23;三孔沟或三合沟孔,内孔一般纵长,具皱波状纹饰,在皱波上具密集的小瘤或微颗粒。从花粉形态的角度,本文支持把囊萼紫草属从滇紫草属(广义)中分离出来的观点。值得注意的是,在滇紫草属的花粉中首次观察到了一种比较少见且特化的花粉即单极三合沟孔的花粉。     

phbB,phbC基因克隆,序列分析及植物表达载体的构建

利用聚合酶链式反应技术,从真养产碱杆菌Ａｌｃａｌｉｇｅｎｅｓ ｅｕｔｒｏｐｈｕｓ Ｈ１６染色体ＤＮＡ中的主增并克隆了调控聚－β－羧基丁酸生物合成的两个关键酶基因;依赖ＮＡＤＰＨ的乙酰乙酰ＣｏＡ还原酶基因和ＰＨＢ合成酶基因。限制性内切酶图谱和核苷酸序列分析证实了克隆结果,并表明所克隆的基因与国外报道的有很高的同源性。     

Human DNA helicase IV is nucleolin, an RNA helicase modulated by phosphorylation

The cDNA encoding human DNA helicase IV (HDH IV), a 100-kDa protein which unwinds DNA in the 5′ to 3′ direction with respect to the bound strand, was cloned and sequenced. It was found to be identical to the human cDNA encoding nucleolin, a ubiquitous eukaryotic protein essential for pre-ribosome assembly. HDH IV/nucleolin can unwind RNA-RNA duplexes, as well as DNA-DNA and DNA-RNA duplexes. Phosphorylation of HDH IV/nucleolin by cdc2 kinase and casein kinase II enhanced its unwinding activity in an additive way. The Gly-rich C-terminal domain possesses a limited ATP-dependent duplex-unwinding activity which contributes to the helicase activity of HDH IV/nucleolin.     

甘肃莲花山及其邻近地区大型真菌调查初报

本文报道了甘肃莲花山及其邻近地区的大型真茵１１７种,根据Ａｉｎｓｗｏｒｔｈ,Ｇ．Ｃ．系统（１９７３）,隶属于６０屑,２６科,２亚门,其中食用菌７０种,药用菌３７种,毒蘑菇２３种,菌根菌２４种,甘肃分布新纪录５０种。