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排序方式: 共有274条查询结果,搜索用时 31 毫秒
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Turbidimetric analysis of growth kinetics of bacteria in the laboratory environment using smartphone
For different microbiological and pathological studies, it is often required to monitor the growth of bacteria in a cultured medium in the laboratory environment. UV‐VIS spectrophotometer is commonly used to estimate the growth of bacterial cell population by measuring the absorbance at 600 nm over a period of time. Colony‐forming unit (CFU) is another approach, which has been routinely performed to estimate the live bacterial cells on semisolid agar plates. Herein, we demonstrate an alternative yet highly reliable sensing platform on a smartphone using which growth kinetics of different bacteria can be reliably monitored. The performance of the proposed smartphone sensor has been compared with the data obtained from OD600 and CFU analysis. A good correlation of bacterial growth rates enumerated based on the proposed smartphone sensor, bench‐top spectrophotometer and CFU analysis have been observed under the experimental conditions. 相似文献
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Lars J. von Buchholtz Nima Ghitani Ruby M. Lam Julia A. Licholai Alexander T. Chesler Nicholas J.P. Ryba 《Neuron》2021,109(2):285-298.e5
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265.
Nima Badie 《Biophysical journal》2009,96(9):3873-3885
Systematic studies of cardiac structure-function relationships to date have been hindered by the intrinsic complexity and variability of in vivo and ex vivo model systems. Thus, we set out to develop a reproducible cell culture system that can accurately replicate the realistic microstructure of native cardiac tissues. Using cell micropatterning techniques, we aligned cultured cardiomyocytes at micro- and macroscopic spatial scales to follow local directions of cardiac fibers in murine ventricular cross sections, as measured by high-resolution diffusion tensor magnetic resonance imaging. To elucidate the roles of ventricular tissue microstructure in macroscopic impulse conduction, we optically mapped membrane potentials in micropatterned cardiac cultures with realistic tissue boundaries and natural cell orientation, cardiac cultures with realistic tissue boundaries but random cell orientation, and standard isotropic monolayers. At 2 Hz pacing, both microscopic changes in cell orientation and ventricular tissue boundaries independently and synergistically increased the spatial dispersion of conduction velocity, but not the action potential duration. The realistic variations in intramural microstructure created unique spatial signatures in micro- and macroscopic impulse propagation within ventricular cross-section cultures. This novel in vitro model system is expected to help bridge the existing gap between experimental structure-function studies in standard cardiac monolayers and intact heart tissues. 相似文献
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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) gains access to host cells by attaching to angiotensin-converting enzyme 2 (ACE2). Vitamin D (VitD) can upregulate ACE2 and has an antagonistic effect on Renin, which exerts a vasodilatation and anti-inflammatory effect against coronavirus disease (COVID-19). However, it may also facilitate viral entry by increasing ACE2 as the main SARS-CoV-2 receptor and mediates ROS production through NADPH oxidase, as a double-edged sword effect. Lung function and the immune system are also influenced by VitD through several mechanisms, including increased natural antibiotics (Defensin and Cathelicidin) and upregulated transforming growth factor-β. A higher IgA, Th2/Th1 ratio, and T-regulatory cells are attributable to VitD effects on the immune cells, while these changes may also be a double-edged sword in COVID-19. Although VitD supplementation might be highly recommended in COVID-19, the administration's dosage and route could be challenging. Furthermore, this issue has not been mentioned in various studies so far. So, the report aimed to explain the current challenges with the application of VitD in COVID-19. 相似文献
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In vivo electrochemistry has been a valuable tool in detecting real time neurochemical changes in extracellular fluid. Absolute selectivity has been difficult to achieve previously, but we report here a carbon fiber electrode and measurement technique which is specific for one oxidizable species: ascorbic acid. Ascorbic acid is highly concentrated in extra- as well as intracellular brain spaces, and appears to undergo dynamic changes in response to a variety of physiological and pathophysiological circumstances. Recent studies have implicated glutamatergic mechanisms which give rise to extracellular changes in brain ascorbate, and we confirm and extend these observations. Preliminary studies, directed towards examining ascorbic acid as an index and/or result of hypoxia, spreading depression, and seizure activity, have been undertaken and the results are reported herein.Special issue dedicated to Dr. Frederick E. Samson. 相似文献
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Sadrishojaei Mahyar Navimipour Nima Jafari Reshadi Midia Hosseinzadeh Mehdi 《Cluster computing》2022,25(1):351-361
Cluster Computing - Internet of things has become an essential principle of human life with the widespread acceptance of intelligent environments, where everyday objects can communicate through the... 相似文献
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