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901.
Summary The hypotheses suggested by Chitty (1960) and by Charnov and Finerty (1980) in order to explain the occurrence of the microtine density cycle are investigated from a theoretical point of view. The former hypothesis assumes that the polymorphic behavioural patterns being observed in natural populations are genetically determined whereas the latter assumes these polymorphic behavioural patterns to be environmentally determined. For both theories we assume a patchy environment with dispersal between patches.We find, on the basis of a series of mathematical models comparing the two hypotheses, that Chitty's hypothesis does not seem to generate cycles whereas the Charnov-Finerty hypothesis may generate persistent density cycles: this is particularly so if dispersing individuals have the capacity to choose which patch to settle in and if the carrying capacity of each patch is high.Work carried out while HWD was visiting the Dept. of Biology, Division of Zoology, University of Oslo on a Norwegian Government Scholarship 相似文献
902.
Morten Juhl Corydon Brage Storstein Andresen Peter Bross Margrethe Kjeldsen Per Hove Andreasen Hans Eiberg Steen Kølvraa Nils Gregersen 《Mammalian genome》1997,8(12):922-926
Short-chain acyl-CoA dehydrogenase (SCAD) is a homotetrameric mitochondrial flavoenzyme that catalyzes the initial reaction
in short-chain fatty acid β-oxidation. Defects in the SCAD enzyme are associated with failure to thrive, often with neuromuscular
dysfunction and elevated urinary excretion of ethylmalonic acid (EMA). To define the genetic basis of SCAD deficiency and
ethylmalonic aciduria in patients, we have determined the sequence of the complete coding portion of the human SCAD gene (ACADS)
and all of the intron-exon boundaries. The SCAD gene is approximately 13 kb in length and consists of 10 exons. Four polymorphic
sites have previously been detected by sequencing of cDNA from fibroblasts of patients excreting elevated amounts of EMA.
Three of these polymorphisms (321T/C, 990C/T, 1260G/C) are silent variants, while a 625G/A polymorphism results in an amino
acid replacement and has been shown to be associated with ethylmalonic aciduria. From analysis of 18 unrelated Danish families,
we show that the four SCAD gene polymorphisms constitute five allelic variants of the SCAD gene, and that the 625A variant
together with the less frequent variant form of the three other polymorphisms (321C, 990T, 1260C) constitutes an allelic variant
with a frequency of 22% in the general Danish population. Using fluorescence in-situ hybridization, we confirm the localization
of the human SCAD gene to the distal part of Chromosome (Chr) 12 and suggest that the SCAD gene is a single-copy gene. The
evolutionary relationship between SCAD and five other members of the acyl-CoA dehydrogenase family was investigated by two
independent approaches that gave similar phylogenetic trees.
Received: 10 April 1997 / Accepted: 8 August 1997 相似文献
903.
The catalytic mechanism of Pseudomonas cytochrome c peroxidase 总被引:1,自引:0,他引:1
Marjaana Rönnberg Tsunehisa Araiso Nils Ellfolk H.Brian Dunford 《Archives of biochemistry and biophysics》1981,207(1):197-204
The catalytic mechanism of Pseudomonas cytochrome c peroxidase has been studied using rapid-scan spectrometry and stopped-flow measurements. The reaction of the totally ferric form of the enzyme with H2O2 was slow and the complex formed was inactive in the peroxidatic cycle, whereas partially reduced enzyme formed highly reactive intermediates with hydrogen peroxide. Rapid-scan spectrometry revealed two different spectral forms, one assignable to Compound I and the other to Compound II as found in the reaction cycle of other peroxidases. The formation of Compound I was rapid approaching that of diffusion control. The stoichiometry of the peroxidation reaction, deduced from the formation of oxidized electron donor, indicates that both the reduction of Compound I to Compound II and the conversion of Compound II to resting (partially reduced) enzyme are one-electron steps. It is concluded that the reaction mechanism generally accepted for peroxidases is applicable also to Pseudomonas cytochrome c peroxidase, the intramolecular source of one electron in Compound I formation, however, being reduced heme c. 相似文献
904.
905.
Programmed cell death contributes to the histogenesis of the nervous system, and is believed to be modulated through the sustaining effects of afferents and targets during the period of synaptogenesis. Cone bipolar cells undergo programmed cell death during development, and we confirm that the numbers of three different types are increased when the pro-apoptotic Bax gene is knocked out. When their cone afferents are selectively eliminated, or when the population of retinal ganglion cells is increased, however, cone bipolar cell number remains unchanged. Programmed cell death of the cone bipolar cell populations, therefore, may be modulated cell-intrinsically rather than via interactions with these synaptic partners. 相似文献
906.
Nils Chr. Stenseth 《Oecologia》1978,33(2):149-172
Summary A graphic model for individual selection determined by the logarithmic growth rates, dN
i
/N
i
·dt, is developed for studying optimal demographic strategies at different phases of microtine cycles. In a density-independent situation (no crowding) selection leads to maximization of total life-time reproductive output (or equivalently, the Malthusian parameter, m) at the expense of competitive (contest type) abilities. In a density-dependent situation (crowding), selection leads to maximization of contest type competitive abilities at the expense of reproductive output. These two modes of selective pressure are called r- and -selection. r-selection is presumed to occur during the increase phase of a cycle. As the habitat becomes crowded, -selection takes over and is assumed to be extreme at high densities.The characteristics of r- and -strategists are found to be similar to those of the docile and aggressive forms presumed in Chitty's theory for fluctuating populations. Literature supports the attributes predicted by the theory. I argue that sensitivity to density-independent factors is higher in the -strategy. On the basis of a graphic model, I show that the -strategists' high sensitivity to extrinsic factors will account for the crash in microtine cycles.On the basis of these theoretical considerations, Chitty's theory for fluctuating populations is interpreted to imply that interactions between intrinsic and extrinsic (random) factors will result in cycles. A graphic model for this interpretation of Chitty's theory is discussed. The heterogeneity of the habitat is an important aspect.According to theory, dispersal of pregnant females is explained as an adaptation leading to increased current reproductive output. This behaviour is presumed to dominate numerically during the increase phase of a cycle, a prediction supported in literature. 相似文献
907.
Adrenomedullin and PAMP are potent vasodilatory peptides derived from a common larger precursor peptide. Elevation of circulating levels of both peptides has been described for diseases involving dysfunction of the cardiovascular system. However, the reliable quantification has been hampered by their short half-life times and - as known for Adrenomedullin -- the existence of a binding protein. Here we report the identification of another peptide derived from the Adrenomedullin precursor, termed proADM 45-92, which is present in large concentrations in plasma of septic shock patients. This peptide is produced in stoichiometric amounts to Adrenomedullin and PAMP, but -- contrary to them -- is apparently non-functional and stable. Thus, proADM 45-92 represents a suitable diagnostic target which could be used to assess the concentrations of Adrenomedullin gene products released into the bloodstream. 相似文献
908.
Daniela Schulte Timothy J. Close Andreas Graner Peter Langridge Takashi Matsumoto Gary Muehlbauer Kazuhiro Sato Alan H. Schulman Robbie Waugh Roger P. Wise Nils Stein 《Plant physiology》2009,149(1):142-147
Arabidopsis (Arabidopsis thaliana) tryptophan-proline-proline (WPP)-domain proteins, WPP1 and WPP2, are plant-unique, nuclear envelope-associated proteins of unknown function. They have sequence similarity to the nuclear envelope-targeting domain of plant RanGAP1, the GTPase activating protein of the small GTPase Ran. WPP domain-interacting tail-anchored protein 1 (WIT1) and WIT2 are two Arabidopsis proteins containing a coiled-coil domain and a C-terminal predicted transmembrane domain. They are required for RanGAP1 association with the nuclear envelope in root tips. Here, we show that WIT1 also binds WPP1 and WPP2 in planta, we identify the chaperone heat shock cognate protein 70-1 (HSC70-1) as in vivo interaction partner of WPP1 and WPP2, and we show that HSC70-1 interacts in planta with WIT1. WIT1 and green fluorescent protein (GFP)-WIT1 are targeted to the nuclear envelope in Arabidopsis. In contrast, GFP-WIT1 forms large cytoplasmic aggregates when overexpressed transiently in Nicotiana benthamiana leaf epidermis cells. Coexpression of HSC70-1 significantly reduces GFP-WIT1 aggregation and permits association of most GFP-WIT1 with the nuclear envelope. Significantly, WPP1 and WPP2 show the same activity. A WPP1 mutant with reduced affinity for GFP-WIT1 fails to decrease its aggregation. While the WPP-domain proteins act on a region of WIT1 containing the coiled-coil domain, HSC70-1 additionally acts on the C-terminal transmembrane domain. Taken together, our data suggest that both HSC70-1 and the WPP-domain proteins play a role in facilitating WIT1 nuclear envelope targeting, which is, to our knowledge, the first described in planta activity for the WPP-domain proteins.The cytoplasmic Ran GTPase activating protein RanGAP is critical to establishing a functional RanGTP/RanGDP gradient across the nuclear envelope (NE) and is associated with the outer surface of the NE in metazoan and higher plant cells (Matunis et al., 1996; Rose and Meier, 2001). Plant RanGAP1 association with the NE requires a plant-specific targeting domain, named the Trp-Pro-Pro (WPP) domain (Rose and Meier, 2001). Arabidopsis (Arabidopsis thaliana) WPP1 and WPP2 are small (155- and 180-amino-acid residues, respectively) plant-unique proteins of unknown function, which are similar to the WPP domain of RanGAP proteins. WPP1 and WPP2 are located in the cytoplasm, with a concentration at the NE (Patel et al., 2004). They are characterized by a 104-amino-acid-long WPP domain, predicted to consist of a β-strand and three α-helices and shown to be sufficient for NE targeting (Patel et al., 2004). They are also associated with cytoplasmic speckles most likely representing Golgi (Patel et al., 2005). Reduced expression of the WPP protein family causes decreased mitotic activity in roots of Arabidopsis, resulting in shortening of primary roots and decreased number of lateral roots (Patel et al., 2004). RanGAP1 association with the NE in the Arabidopsis root tip requires two families of NE-localized, plant-specific, WPP domain-interacting proteins (WPP domain-interacting protein [WIP] and WPP domain-interacting tail-anchored protein [WIT] families) that are characterized by the presence of a coiled-coil domain and a C-terminal predicted transmembrane domain (TMD; Xu et al., 2007; Zhao et al., 2008). Based on sequence analysis, both the WIP and WIT protein family were classified as putative tail-anchored (TA) proteins, proteins that associate with membranes posttranslationally (Borgese et al., 2003).The heat shock protein 70 family (HSP70) contains both heat-inducible and constitutively expressed members, called heat shock cognate proteins (HSC70). HSC70 chaperones assist in folding newly synthesized proteins (Bukau and Horwich, 1998), are involved in posttranslational translocation of secretory proteins across endoplasmic reticulum (ER) and mitochondrial membranes (Chirico et al., 1988; Deshaies et al., 1988), prevent irreversible aggregation of their substrates (Ngosuwan et al., 2003), and facilitate degradation of misfolded proteins (Meacham et al., 2001). Recently, mammalian HSC70 has also been implied in assisting the membrane insertion of a subset of TA proteins (Abell et al., 2007).The Arabidopsis genome encodes five different cytosolic HSP70s, three of which are expressed constitutively (HSC70-1, HSC70-2, and HSC70-3). While expressed in all organs, Hsc70-1 and Hsc70-2 expression levels are highest in leaves and Hsc70-3 in leaves and roots. All three genes can be further induced by heat shock and cold stress (Sung et al., 2001). Constitutive overexpression of Arabidopsis Hsc70-1 in transgenic plants leads to changes in growth and development, increases thermotolerance (Sung and Guy, 2003), and decreases the plant''s ability to respond to pathogen attack (Noel et al., 2007). Recently, specific interactions of HSC70-1 with SGT1 (for Suppressor of G2 allele of skp1; Noel et al., 2007) and HSC70-3 with turnip mosaic virus RNA-dependent RNA polymerase (Dufresne et al., 2008) were identified, suggesting a role of HSC70 in viral replication and pathogenesis. Both HSC70-1 and HSC70-3 can be detected in the nuclei and the cytoplasm of Nicotiana benthamiana epidermal cells (Noel et al., 2007; Dufresne et al., 2008).Here, we identified Arabidopsis HSC70-1 as an in vivo interaction partner of WPP1 and WPP2 and demonstrated that HSC70-1 associates with WIT1. Using transient expression in N. benthamiana, we show that when expressed at a high level, WIT1 accumulates in large fluorescent bodies in the cytoplasm that may represent aggregates. Upon coexpression in the same system, WPP1, WPP2, and HSC70-1 are all able to prevent the aggregation of overexpressed WIT1 and enable WIT1 association with the NE. While WPP-domain proteins act on a region of WIT1 containing the coiled-coil domain, HSC70-1 additionally acts on the C-terminal TMD. We propose that WPP1 and WPP2 play a chaperone-like role reflected in preventing the aggregation of the coiled-coil region of WIT1 and possibly other coiled-coil TA-type proteins, either in conjunction or independently of HSC70-type chaperones. 相似文献
909.
Nils Kurlemann Miguel Lara Martina Pohl Wolfgang Kroutil Andreas Liese 《Journal of Molecular Catalysis .B, Enzymatic》2009,61(1-2):111
Two different biocatalytic reactions – a CC cleavage and a CC forming reaction – were evaluated concerning their application in a reaction sequence. In the overall reaction, an aromatic alkene was converted to a chiral 2-hydroxy ketone. In the first step, the olefin trans-anethole was converted to para-anisaldehyde and acetaldehyde by an aqueous extract of the white rot fungus Trametes hirsuta G FCC 047. The selective oxidative cleavage of the carbon–carbon double bond was achieved using molecular oxygen as a substrate. In a second step p-anisaldehyde was ligated to acetaldehyde to yield either (R)- or (S)-2-hydroxy-1-(4-methoxyphenyl)-propanone. The reaction was catalyzed by the enantiocomplementary CC bond forming enzymes benzaldehyde lyase and benzoylformate decarboxylase, respectively. 相似文献
910.
In the present study we compared the contents of water soluble organic compounds of the marine intertidal ecotype of Fucus vesiculosus (Phaeophyceae) from the Norwegian Sea (34 practical salinity units, psu) with the sublittoral ecotype of F. vesiculosus from the brackish Bothnian Sea (5 psu). Nuclear magnetic resonance spectra revealed that marine F. vesiculosus had additional types of water soluble organic compounds compared with brackish F. vesiculosus. The results suggested that glycine betaine in the marine ecotype could be the reason for this ecotype differentiation. Furthermore, the qualitative differences between the ecotypes were the same after one week's treatment of marine algae in brackish water and of brackish algae in marine water. These suggest that the additional types of water soluble organic compounds in marine F. vesiculosus are not caused by the salinity conditions at the growth sites. Further research concerning other environmental factors that may influence ecotype differentiation of water soluble organic compounds qualitative content and adaptation in F. vesiculosus is recommended. 相似文献