全文获取类型
收费全文 | 474篇 |
免费 | 41篇 |
出版年
2024年 | 2篇 |
2023年 | 2篇 |
2022年 | 7篇 |
2021年 | 14篇 |
2020年 | 10篇 |
2019年 | 6篇 |
2018年 | 10篇 |
2017年 | 8篇 |
2016年 | 18篇 |
2015年 | 23篇 |
2014年 | 20篇 |
2013年 | 28篇 |
2012年 | 56篇 |
2011年 | 31篇 |
2010年 | 23篇 |
2009年 | 24篇 |
2008年 | 37篇 |
2007年 | 22篇 |
2006年 | 14篇 |
2005年 | 18篇 |
2004年 | 12篇 |
2003年 | 13篇 |
2002年 | 12篇 |
2001年 | 11篇 |
2000年 | 9篇 |
1999年 | 6篇 |
1998年 | 3篇 |
1997年 | 2篇 |
1996年 | 7篇 |
1993年 | 3篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1990年 | 4篇 |
1989年 | 5篇 |
1988年 | 4篇 |
1987年 | 5篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1980年 | 3篇 |
1979年 | 4篇 |
1977年 | 5篇 |
1975年 | 1篇 |
1974年 | 6篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1971年 | 2篇 |
1969年 | 3篇 |
1968年 | 1篇 |
1967年 | 3篇 |
1963年 | 1篇 |
排序方式: 共有515条查询结果,搜索用时 15 毫秒
101.
Pankaj Thapa Nilesh Shanmugam Wojciech Pokrzywa 《BioEssays : news and reviews in molecular, cellular and developmental biology》2020,42(1):1900171
The fate of eukaryotic proteins, from their synthesis to destruction, is supervised by the ubiquitin–proteasome system (UPS). The UPS is the primary pathway responsible for selective proteolysis of intracellular proteins, which is guided by covalent attachment of ubiquitin to target proteins by E1 (activating), E2 (conjugating), and E3 (ligating) enzymes in a process known as ubiquitylation. The UPS can also regulate protein synthesis by influencing multiple steps of RNA (ribonucleic acid) metabolism. Here, recent publications concerning the interplay between the UPS and different types of RNA are reviewed. This interplay mainly involves specific RNA-binding E3 ligases that link RNA-dependent processes with protein ubiquitylation. The emerging understanding of their modes of RNA binding, their RNA targets, and their molecular and cellular functions are primarily focused on. It is discussed how the UPS adapted to interact with different types of RNA and how RNA molecules influence the ubiquitin signaling components. 相似文献
102.
During a floristic survey in the Belgaum district, Karnataka state, some specimens of Rotala L. were collected from a lateritic plateau in western Ghats, India. The specimens did not match with any previously described species of Rotala, but closely resembled R. macrandra Koehne and R. rosea (Poir.) C. D. K. Cook ex H. Hara in their exserted stamens and styles. The new species is described and illustrated here as Rotala belgaumensis S. R. Yadav, Malpure & Chandore. 相似文献
103.
Paraskevi Christofidou Christopher?P. Nelson Majid Nikpay Liming Qu Mingyao Li Christina Loley Radoslaw Debiec Peter?S. Braund Matthew Denniff Fadi?J. Charchar Ares?Rocanin Arjo David-Alexandre Trégou?t Alison?H. Goodall Francois Cambien Willem?H. Ouwehand Robert Roberts Heribert Schunkert Christian Hengstenberg Muredach?P. Reilly Jeanette Erdmann Ruth McPherson Inke?R. K?nig John?R. Thompson Nilesh?J. Samani Maciej Tomaszewski 《American journal of human genetics》2015,97(2):228-237
Runs of homozygosity (ROHs) are recognized signature of recessive inheritance. Contributions of ROHs to the genetic architecture of coronary artery disease and regulation of gene expression in cells relevant to atherosclerosis are not known. Our combined analysis of 24,320 individuals from 11 populations of white European ethnicity showed an association between coronary artery disease and both the count and the size of ROHs. Individuals with coronary artery disease had approximately 0.63 (95% CI: 0.4–0.8) excess of ROHs when compared to coronary-artery-disease-free control subjects (p = 1.49 × 10−9). The average total length of ROHs was approximately 1,046.92 (95% CI: 634.4–1,459.5) kb greater in individuals with coronary artery disease than control subjects (p = 6.61 × 10−7). None of the identified individual ROHs was associated with coronary artery disease after correction for multiple testing. However, in aggregate burden analysis, ROHs favoring increased risk of coronary artery disease were much more common than those showing the opposite direction of association with coronary artery disease (p = 2.69 × 10−33). Individual ROHs showed significant associations with monocyte and macrophage expression of genes in their close proximity—subjects with several individual ROHs showed significant differences in the expression of 44 mRNAs in monocytes and 17 mRNAs in macrophages when compared to subjects without those ROHs. This study provides evidence for an excess of homozygosity in coronary artery disease in outbred populations and suggest the potential biological relevance of ROHs in cells of importance to the pathogenesis of atherosclerosis. 相似文献
104.
A gene that regulates the hemolysin structural gene (hly) was found to be tightly linked to the tox-1000 locus of Vibrio cholerae RJ1 and separated from hly by a large section of the V. cholerae genetic map. This hemolysin regulatory gene was designated hlyR. 相似文献
105.
Sharma Richa Roopak Sangita Pathak Nilesh kumar Uma R. Sharma R. P. 《Plasmonics (Norwell, Mass.)》2018,13(1):335-343
Plasmonics - In the present work, we report our observations drawn from the numerical simulation of absorption and scattering efficiencies of spheroid shape nanostructures using discrete dipole... 相似文献
106.
P. Sudhakar Babu A. N. Vaidya A. S. Bal Rajesh Kapur Asha Juwarkar P. Khanna 《Biotechnology letters》1996,18(3):263-268
Summary Batch kinetic studies were carried out on rhamnolipid biosurfactant production from synthetic medium, industrial wastes viz. distillery and whey waste as substrates. The results indicated that the specific growth rates ( max) and specific product formation rates (V max) from both the wastes are comparatively better than the synthetic medium, revealing that both the industrial wastes (distillery and whey) can be successfully utilized as substrates for biosurfactant production. 相似文献
107.
Twin-core packed-bed reactors for organic-phase enzymatic esterification with water activity control
A method for the removal of water and the control of water activity, a
w, during enzymatic esterification is the use of salt hydrate pairs. When this technique is used on a laboratory scale, the recovery and reuse of the salt are not critical. Potential problems, such as the reactivity of some salts, can also be overcome simply by substituting another salt. However, if this technique is to be used on a larger scale, economic constraints would require salt recovery and restric the range of salts that could be used. In this article a twin-core packed-bed reactor — used for the esterification of an equimolar mixture of decanoic acid and dodecanol catalysed by lipase from Candida rugosa — which facilitates salt recovery and permits a
w control without direct contact between immobilized enzyme and salt, has been described. a
w control was maintained by using suitable salt hydrate mixtures in the inner core of the reactor. The substrate mixture was esterified by pumping it through the outer core of the reactor, which contained enzyme immobilized on a macroporous polypropylene support. Complete conversion, albeit at different rates, was obtained with a
w buffering at 0.48 and 0.8 by using hydrates of Na4P2O7 and Na2HPO4. 相似文献
108.
Binay K. Singh Dwijesh C. Mishra Sushma Yadav Supriya Ambawat Era Vaidya Kishor U Tribhuvan Arun Kumar Sujith Kumar Sanjeev Kumar K. K. Chaturvedi Reema Rani Prashant Yadav Anil Rai P. K. Rai V. V. Singh Dhiraj Singh 《Journal of plant biochemistry and biotechnology.》2016,25(4):410-420
Brassica juncea is an economically important oilseed crop worldwide. It has limited genomic resources at present. We generated 47,962,057 expressed sequence reads which were assembled into 45,280 unigenes. A total of 4108 SSR loci (≥10 bp) were identified in these unigenes. Trinucleotide was the most frequent repeat unit (59.91 %) followed by di- (38.66 %), tetra - (0.71 %), hexa - (0.49 %) and pentanucleotide repeats (0.24 %). Primers were designed for 2863 SSR loci among which 460 were selected for primer synthesis. A total of 339 loci amplified successfully of which 134 (39.5 %) exhibited polymorphism among six B. juncea genotypes with PIC values ranging from 0.18 to 0.81. Further, 25 polymorphic SSRs were used for analysis of genetic variability in 25 genotypes of Brassicas and their wild relatives. Two to five alleles with PIC values 0.22–0.66 were detected at these loci. The dendrogram grouped the genotypes according to their known pedigree/systematic position. 相似文献
109.
110.