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111.
Terminal loop-mediated control of microRNA biogenesis 总被引:1,自引:0,他引:1
Regulation of miRNA (microRNA) biogenesis shapes the profiles of miRNAs in the living cell, contributing to cell identity and function. Importantly, aberrant miRNA levels have been linked to a variety of human pathological states. In recent years, a number of proteins have been shown to regulate the miRNA biogenesis at the level of Drosha and Dicer cleavage. A large proportion of these factors regulate miRNA production through binding to the TL (terminal loop) regions of miRNA progenitors. In the present paper, we review the current knowledge about pri-miRNA (primary miRNA) and pre-miRNA (precursor miRNA) TL involvement in the regulation of miRNA biogenesis. 相似文献
112.
In vitro mutagenesis of a xylanase from the extreme thermophile Caldocellum saccharolyticum 总被引:1,自引:0,他引:1
Ernst Lüthi Karin Reif Nila Bhana Jasmat Peter L. Bergquist 《Applied microbiology and biotechnology》1992,36(4):503-506
Summary Six mutant xylanases were obtained by in vitro mutagenesis of a xylanase gene from the extremely thermophilic bacterium Caldocellum saccharolyticum. The temperature stability of all enzymes was affected by mutation to various degrees and one of the xylanases had an altered temperature optimum. The mutations had no effect on the pH optimum. The C. saccharolyticum xylanase showed strong homology to several thermophilic and mesophilic xylanases, and comparison of primary sequences allowed the localization of probable active sites and residues involved in thermostability.
Offprint requests to: P. L. Bergquist 相似文献
113.
Volpi N 《Analytical biochemistry》2000,277(1):19-24
A system capable of resolving all the known unsaturated nonsulfated, mono- and disulfated disaccharides derived from chondroitin sulfate samples, dermatan sulfate, and hyaluronic acid after their derivatization with dansylhydrazine and separation by HPLC and fluorimetric detection is reported. This method was found superior to others in that unsaturated disaccharides can be separated with good resolution in about 50 min in an isocratic solvent with a sensitivity greater than about 50 pmol (approx 20-30 ng) and linearity from 50 to 500 pmol. The system was applied to the analysis of various chondroitin sulfate samples, including highly sulfated species and dermatan sulfate, and also to a defructosylated polysaccharide with a chondroitin backbone purified from Escherichia coli U1-41. Excellent agreement was obtained with traditional compositional analysis performed by anion-exchange HPLC separation and UV absorption at 230 nm. 相似文献
114.
Nicola Volpi Lucia Zampini Francesca Maccari Lucia Santoro Fabio Galeotti Tiziana Galeazzi Orazio Gabrielli Giovanni V. Coppa 《Glycoconjugate journal》2013,30(7):727-732
Enzyme replacement therapy (ERT) is the worldwide standard of care for a number of mucopolysaccharidosis (MPS) diseases. We report a kinetic study of plasmatic dermatan sulfate (DS) in a 3-year-old subject affected by a severe form of MPS II during the first 10 months of ERT with Idursulfase. A strong increase in the DS plasmatic concentration was measured immediately after the first enzyme infusion, with a maximum after 3 h, followed by a continuous decrease in the 8–15 days following the beginning of treatment. After this, a constant plasmatic content of DS concentration was observed. Overall, during the 10-month treatment period, ERT reduced the plasmatic concentration of DS up to ~80–85 %, but it was unable to totally remove it from the blood. We can suppose that immediately after the first enzyme administrations, a large amount of abnormal DS is removed from tissues reaching the blood compartment and eliminated via the urine, and thereafter only minimal changes are observed. The persistency of the residual amounts of DS with the actually recommended dosage in our Patient may suggest the opportunity to promote further studies with increased enzyme dosages to completely remove the accumulation of lysosomal DS. 相似文献
115.
Rebecca W.Y. Chan Lee Serpas Meng Ni Stefano Volpi Linda T. Hiraki Lai-Shan Tam Ali Rashidfarrokhi Priscilla C.H. Wong Lydia H.P. Tam Yueyang Wang Peiyong Jiang Alice S.H. Cheng Wenlei Peng Diana S.C. Han Patty P.P. Tse Pik Ki Lau Wing-Shan Lee Alberto Magnasco Elisa Buti Vanja Sisirak Nora AlMutairi K.C. Allen Chan Rossa W.K. Chiu Boris Reizis Y.M. Dennis Lo 《American journal of human genetics》2020,107(5):882
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Samantha J. Griffiths Manfred Koegl Chris Boutell Helen L. Zenner Colin M. Crump Francesca Pica Orland Gonzalez Caroline C. Friedel Gerald Barry Kim Martin Marie H. Craigon Rui Chen Lakshmi N. Kaza Even Fossum John K. Fazakerley Stacey Efstathiou Antonio Volpi Ralf Zimmer Peter Ghazal Jürgen Haas 《PLoS pathogens》2013,9(8)
119.
Francesca Schena Stefano Volpi Caterina Elisa Faliti Federica Penco Spartaco Santi Michele Proietti Ursula Schenk Gianluca Damonte Annalisa Salis Marta Bellotti Franco Fais Claudya Tenca Marco Gattorno Hermann Eibel Marta Rizzi Klaus Warnatz Marco Idzko Cemil Korcan Ayata Elisabetta Traggiai 《Cell reports》2013,3(6):1824-1831
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120.