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71.
72.
AG. van Ginkel BJ. Sorgdrager M. A. de Graaf I. Karalis N. Ajmone Marsan 《Netherlands heart journal》2014,22(2):77-79
We report a case of an allergic reaction after the administration of an echocardiographic contrast agent which resulted in ST-segment elevation. Hypersensitivity and allergic reactions are known causes of acute cardiovascular events. However, only limited reports are available which suggest the exact mechanism of the occurrence of angina or myocardial infarction during severe allergic reactions. In our case, through invasive imaging (coronary angiography and IVUS) we have shown for the first time a transient coronary spasm in the absence of intra-coronary thrombus and only minimal neointimal hyperplasia. 相似文献
73.
Adelmo L Cechin Marialva Sinigaglia Ney Lemke Sérgio Echeverrigaray Odalys G Cabrera Gonçalo AG Pereira José CM Mombach 《BMC plant biology》2008,8(1):50
Background
NEP1-like proteins (NLPs) are a novel family of microbial elicitors of plant necrosis. Some NLPs induce a hypersensitive-like response in dicot plants though the basis for this response remains unclear. In addition, the spatial structure and the role of these highly conserved proteins are not known. 相似文献74.
75.
Human lens α-crystallin becomes progressively insoluble with age and is the major crystallin component in the water-insoluble
(WI) fraction. The mechanism that causes the originally water-soluble (WS) α-crystallin to become insoluble is unknown. A
conformational change by chemical modification may be the cause, but the nature of insolubility renders it impossible to study
protein conformation in the WI fraction by most spectroscopic measurements. In the present study, α-crystallin in the WI fraction
was extracted by urea and reconstituted to a folded protein by dialysis. The refolded urea-soluble (US) α-crystallin was compared
with WS α-crystallin. The US α-crystallin has a greater amount of polymeric species, but fewer degraded subunits than the
WS α-crystallin as shown by SDS-PAGE and Western blot. Circular dichroism (CD) measurements indicate that they have the same
secondary structure but a different tertiary structure, possibly a partial unfolding in the US α-crystallin. This is supported
by fluorescence measurements: Trp residues are more exposed and protein has a more-hydrophobic surface in the US than in the
WS α-crystallin. Blue fluorescence further indicates that the US α-crystallin has a greater amount of pigment than the WS
α-crystallin. Together, these results indicate that the US α-crystallin is a chemically and conformationally modified protein. 相似文献
76.
Industrial biotechnology employs the controlled use of microorganisms for the production of synthetic chemicals or simple
biomass that can further be used in a diverse array of applications that span the pharmaceutical, chemical and nutraceutical
industries. Recent advances in metagenomics and in the incorporation of entire biosynthetic pathways into Saccharomyces cerevisiae have greatly expanded both the fitness and the repertoire of biochemicals that can be synthesized from this popular microorganism.
Further, the availability of the S. cerevisiae entire genome sequence allows the application of systems biology approaches for improving its enormous biosynthetic potential.
In this review, we will describe some of the efforts on using S. cerevisiae as a cell factory for the biosynthesis of high-value natural products that belong to the families of isoprenoids, flavonoids
and long chain polyunsaturated fatty acids. As natural products are increasingly becoming the center of attention of the pharmaceutical
and nutraceutical industries, the use of S. cerevisiae for their production is only expected to expand in the future, further allowing the biosynthesis of novel molecular structures
with unique properties. 相似文献