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Konstantinos Gkagkavouzis Nikoleta Karaiskou Theodora Katopodi Ioannis Leonardos Theodore J. Abatzopoulos Alexander Triantafyllidis 《Journal of fish biology》2019,94(4):606-613
We examined 662 gilthead sea bream Sparus aurata from wild samples of the species in the Aegean and Ionian Seas, using 20 EST-linked microsatellite markers, in three multiplex panels, as well as seven anonymous loci. Most of the markers were revealed to be highly polymorphic. We found low genetic differentiation between the sampling stations/areas with total FST 0.002 (P < 0.05). Based on comparison of five temporal samples, our results indicate genetic data consistency over time for all tested samples, pointing to stable populations, despite reported repeated escape events. Our results confirm the genetic population structure previously observed in these specific areas, using by far more markers than in previous studies in both coding and non-coding DNA loci. The limited genetic structure and the temporal genetic stability indicate neither major genetic differentiation of local populations by geographic isolation nor influence from anthropogenic factors. These results provide a baseline for future reference in any management programme of both wild and farmed population of S. aurata as well as of other aquaculture species with a potential introgression among farmed and wild populations. 相似文献
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Charilaos Pylidis Peeter Anijalg Urmas Saarma Deborah A. Dawson Nikoleta Karaiskou Roger Butlin Yorgos Mertzanis Alexios Giannakopoulos Yorgos Iliopoulos Andrew Krupa Terence A. Burke 《Ecology and evolution》2021,11(11):6427
In human‐dominated landscapes, connectivity is crucial for maintaining demographically stable mammalian populations. Here, we provide a comprehensive noninvasive genetic study for the brown bear population in the Hellenic Peninsula. We analyze its population structuring and connectivity, estimate its population size throughout its distribution, and describe its phylogeography in detail for the first time. Our results, based on 150 multilocus genotypes and on 244‐bp sequences of the mtDNA control region, show the population is comprised by three highly differentiated genetic clusters, consistent with geographical populations of Pindos, Peristeri, and Rhodope. By detecting two male bears with Rhodopean ancestry in the western demes, we provide strong evidence for the ongoing genetic connectivity of the geographically fragmented eastern and western distributions, which suggests connectivity of the larger East Balkan and Pindos‐Dinara populations. Total effective population size (N e) was estimated to be 199 individuals, and total combined population size (N C) was 499, with each cluster showing a relatively high level of genetic variability, suggesting that migration has been sufficient to counteract genetic erosion. The mtNDA results were congruent with the microsatellite data, and the three genetic clusters were matched predominantly with an equal number of mtDNA haplotypes that belong to the brown bear Western mitochondrial lineage (Clade 1), with two haplotypes being globally new and endemic. The detection of a fourth haplotype that belongs to the Eastern lineage (Clade 3a1) in three bears from the western distribution places the southernmost secondary contact zone between the Eastern and Western lineages in Greece and generates new hypotheses about postglacial maxima migration routes. This work indicates that the genetic composition and diversity of Europe''s low‐latitude fringe population are the outcome of ancient and historical events and highlight its importance for the connectivity and long‐term persistence of the species in the Balkans. 相似文献
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The benefits of ever-growing numbers of sequenced eukaryotic genomes will not be fully realized until we learn to decipher vast stretches of noncoding DNA, largely composed of transposable elements. Transposable elements persist through self-replication, but some genes once encoded by transposable elements have, through a process called molecular domestication, evolved new functions that increase fitness. Although they have conferred numerous adaptations, the number of such domesticated transposable element genes remains unknown, so their evolutionary and functional impact cannot be fully assessed. Systematic searches that exploit genomic signatures of natural selection have been employed to identify potential domesticated genes, but their predictions have yet to be experimentally verified. To this end, we investigated a family of domesticated genes called MUSTANG (MUG), identified in a previous bioinformatic search of plant genomes. We show that MUG genes are functional. Mutants of Arabidopsis thaliana MUG genes yield phenotypes with severely reduced plant fitness through decreased plant size, delayed flowering, abnormal development of floral organs, and markedly reduced fertility. MUG genes are present in all flowering plants, but not in any non-flowering plant lineages, such as gymnosperms, suggesting that the molecular domestication of MUG may have been an integral part of early angiosperm evolution. This study shows that systematic searches can be successful at identifying functional genetic elements in noncoding regions and demonstrates how to combine systematic searches with reverse genetics in a fruitful way to decipher eukaryotic genomes. 相似文献
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A gene-based genetic linkage map of the collared flycatcher (Ficedula albicollis) reveals extensive synteny and gene-order conservation during 100 million years of avian evolution
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Backström N Karaiskou N Leder EH Gustafsson L Primmer CR Qvarnström A Ellegren H 《Genetics》2008,179(3):1479-1495
By taking advantage of a recently developed reference marker set for avian genome analysis we have constructed a gene-based genetic map of the collared flycatcher, an important "ecological model" for studies of life-history evolution, sexual selection, speciation, and quantitative genetics. A pedigree of 322 birds from a natural population was genotyped for 384 single nucleotide polymorphisms (SNPs) from 170 protein-coding genes and 71 microsatellites. Altogether, 147 gene markers and 64 microsatellites form 33 linkage groups with a total genetic distance of 1787 cM. Male recombination rates are, on average, 22% higher than female rates (total distance 1982 vs. 1627 cM). The ability to anchor the collared flycatcher map with the chicken genome via the gene-based SNPs revealed an extraordinary degree of both synteny and gene-order conservation during avian evolution. The great majority of chicken chromosomes correspond to a single linkage group in collared flycatchers, with only a few cases of inter- and intrachromosomal rearrangements. The rate of chromosomal diversification, fissions/fusions, and inversions combined is thus considerably lower in birds (0.05/MY) than in mammals (0.6-2.0/MY). A dearth of repeat elements, known to promote chromosomal breakage, in avian genomes may contribute to their stability. The degree of genome stability is likely to have important consequences for general evolutionary patterns and may explain, for example, the comparatively slow rate by which genetic incompatibility among lineages of birds evolves. 相似文献
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Sandra Chanraud Claire Leroy Catherine Martelli Nikoleta Kostogianni Fran?oise Delain Henri-Jean Aubin Michel Reynaud Jean-Luc Martinot 《PloS one》2009,4(8)
Even though uncomplicated alcoholics may likely have episodic memory deficits, discrepancies exist regarding to the integrity of brain regions that underlie this function in healthy subjects. Possible relationships between episodic memory and 1) brain microstructure assessed by magnetic resonance diffusion tensor imaging (DTI), 2) brain volumes assessed by voxel-based morphometry (VBM) were investigated in uncomplicated, detoxified alcoholics.Diffusion and morphometric analyses were performed in 24 alcohol dependent men without neurological or somatic complications and in 24 healthy men. The mean apparent coefficient of diffusion (ADC) and grey matter volumes were measured in the whole brain. Episodic memory performance was assessed using a French version of the Free and Cued Selective Reminding Test (FCSRT). Correlation analyses between verbal episodic memory, brain microstructure, and brain volumes were carried out using SPM2 software.In those with alcohol dependence, higher ADC was detected mainly in frontal, temporal and parahippocampal regions, and in the cerebellum. In alcoholics, regions with higher ADC typically also had lower grey matter volume. Low verbal episodic memory performance in alcoholism was associated with higher mean ADC in parahippocampal areas, in frontal cortex and in the left temporal cortex; no correlation was found between regional volumes and episodic memory scores. Regression analyses for the control group were not significant.These findings support the hypothesis that regional microstructural but no macrostructural alteration of the brain might be responsible, at least in part, for episodic memory deficits in alcohol dependence. 相似文献
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Apostolos P. Apostolidis Andreas Georgiadis Nikoletta Karaiskou Raphael Sandaltzopoulos 《Hydrobiologia》2008,614(1):401-404
We present a reliable, time-saving, and cost-effective multiplex PCR assay for discriminating congeneric species. Three fish
species of the genus Trachurus and two of the genus Mullus served as model cases. Our multiplex PCR method interrogates species-specific diagnostic mitochondrial single nucleotide
polymorphisms (mtSNPs). We selected two sets of mtSNPs that are organized in two multiplex systems for the Trachurus and the Mullus species, respectively. In both systems, all individuals tested could be safely assigned to one of the three Trachurus or two Mullus species. This novel SNP typing system offers a convenient and robust DNA profiling system suitable for large-scale identification
of commercial fish species, for species with cryptic larvae or during juvenile stages, as well as for wildlife forensics.
Handling editor: Christian Sturmbauer 相似文献
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Despoina Vokou Panayiotis G. Dimitrakopoulos Nikoleta Jones Athanasios Damialis Nikos Monokrousos John D. Pantis Antonios D. Mazaris 《Biodiversity and Conservation》2014,23(11):2833-2855
The present study attempts an evaluation of the first co-management framework that has been adopted and implemented in Greece over the last 10 years for the management of its protected areas. To get insight as to how efficient it has been, we evaluate the performance and outcomes of the 28 management authorities of the protected areas of the country that substantiate it and the conditions under which they have been operating. The study involved a large part of the Greek conservation community. It was conducted via a questionnaire dealing with issues of financing and administration, environmental management and guarding, and connection with the local community. For co-management, it is essential that the actors involved undertake their share of responsibility, which should be clearly defined and delimited; this was not usually the case. Decentralization of the power of management requires capacity building locally and active involvement of the local community; these have been addressed and achieved only to a limited degree. Most importantly, the support and commitment to conservation of state actors were often missing. Funding discontinuities, delays in responding to needs associated with biodiversity monitoring and protection and inefficient guarding were the major problems resulting from the inadequacies detected. Despite its weaknesses, the co-management framework contributed considerably to the conservation of environmental values of Greece. We propose improvements and measures that can substantially increase its overall effectiveness towards nature conservation. However, under the severe crisis that the country has been facing, its future is currently unknown. 相似文献
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Nikoleta Karaiskou Paloma Moran George Georgitsakis Theodore J. Abatzopoulos Alexander Triantafyllidis 《Hydrobiologia》2010,638(1):11-20
Genetic polymorphism and differentiation in wild and cultured sea bream samples were studied after amplification, cloning,
and partial sequence of the major histocompatibility complex (MHC) class II alpha antigen. Forty-one alleles were detected
from 43 unrelated individuals and sequence alignment of the obtained alleles revealed 28 polymorphic sites. High heterozygosity
values and allelic richness were unveiled for both wild and cultured populations. The substitution pattern (dN
/dS = 0.7) is not consistent with the effect of diversifying selection, indicating lower selection pressure on the a2 domain,
as well as that too few advantageous non-synonymous mutations have accumulated as substrate for the diversifying selection
to act. Comparison with previously published results on microsatellite markers suggests that balancing selection in MHC genes
reduces the genetic drift and bottleneck effects that are common in aquaculture and which are known to reduce genetic variation
at neutral markers. The present study stresses that both coding and non-coding loci should be analyzed for designing proper
management strategies. 相似文献