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排序方式: 共有1151条查询结果,搜索用时 15 毫秒
41.
Petrunenko Yury K. Seryodkin Ivan V. Bragina Eugenia V. Soutyrina Svetlana S. Mukhacheva Anna S. Rybin Nikolai N. Miquelle Dale G. 《Acta theriologica》2020,65(2):245-253
Mammal Research - The persistence of wildlife populations largely depends on females successfully rearing young through the earliest, most vulnerable period. During this period, mothers must... 相似文献
42.
Nikolai Siebert Christin Eger Diana Seidel Madlen Jüttner Maxi Zumpe Danilo Wegner 《MABS-AUSTIN》2016,8(3):604-616
Ch14.18 manufactured in Chinese hamster ovary (CHO) cells is currently being evaluated in clinical trials. Short-term infusion (STI) (8–20 h/day; 4–5 days) of 100 mg/m2 ch14.18/CHO (dinutiximab β) per cycle in combination with cytokines is standard treatment of neuroblastoma (NB) patients. As pain is a limiting factor, we investigated a novel delivery method by continuous long-term infusion (LTI) of 100 mg/m2 over 10 days. 53 NB patients were treated with 5–6 cycles of 6 × 106 IU/m2 subcutaneous interleukin-2 (d 1-5, 8-12), LTI of 100 mg/m2 ch14.18/CHO (d 8-18) and 160 mg/m2 oral 13-cis-retinoic acid (d 22-35). Human anti-chimeric antibody (HACA), antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity were determined. With LTI, we observed a maximum concentration of ch14.18/CHO (Cmax) of 12.56 ± 0.68 µg/ml and a terminal half-life time (t1/2 β) of 32.7 ± 16.2 d. The clearance values for LTI and STI of 0.54 ± 0.13 and 0.41 ± 0.29 L/d m2 and area under the serum concentration-time curve (AUC) values of 189.6 ± 41.4 and 284.8 ± 156.8 µg×d/ml, respectively, were not significantly different. Importantly, we detected ch14.18/CHO trough concentration of ≥ 1 µg/ml at time points preceding subsequent antibody infusions after cycle 1, allowing a persistent activation of antibody effector mechanisms over the entire treatment period of 6 months. HACA responses were observed in 10/53 (19%) patients, similar to STI (21%), indicating LTI had no effect on the immunogenicity of ch14.18/CHO. In conclusion, LTI of ch14.18/CHO induced effector mechanisms over the entire treatment period, and may therefore emerge as the preferred delivery method of anti-GD2 immunotherapy to NB patients. 相似文献
43.
Zhang Rui Parniakov Oleksii Grimi Nabil Lebovka Nikolai Marchal Luc Vorobiev Eugène 《Bioprocess and biosystems engineering》2019,42(2):173-186
Bioprocess and Biosystems Engineering - Microalgae of Nannochloropsis sp. present valuable source of bio-molecules (pigments, lipids, proteins) that have nutritional potential for the... 相似文献
44.
Kandul NP Lukhtanov VA Pierce NE 《Evolution; international journal of organic evolution》2007,61(3):546-559
That chromosomal rearrangements may play an important role in maintaining postzygotic isolation between well-established species is part of the standard theory of speciation. However, little evidence exists on the role of karyotypic change in speciation itself--in the establishment of reproductive barriers between previously interbreeding populations. The large genus Agrodiaetus (Lepidoptera: Lycaenidae) provides a model system to study this question. Agrodiaetus butterflies exhibit unusual interspecific diversity in chromosome number, from n= 10 to n= 134; in contrast, the majority of lycaenid butterflies have n= 23/24. We analyzed the evolution of karyotypic diversity by mapping chromosome numbers on a thoroughly sampled mitochondrial phylogeny of the genus. Karyotypic differences accumulate gradually between allopatric sister taxa, but more rapidly between sympatric sister taxa. Overall, sympatric sister taxa have a higher average karyotypic diversity than allopatric sister taxa. Differential fusion of diverged populations may account for this pattern because the degree of karyotypic difference acquired between allopatric populations may determine whether they will persist as nascent biological species in secondary sympatry. This study therefore finds evidence of a direct role for chromosomal rearrangements in the final stages of animal speciation. Rapid karyotypic diversification is likely to have contributed to the explosive speciation rate observed in Agrodiaetus, 1.6 species per million years. 相似文献
45.
Wang GL Shi X Salisbury E Sun Y Albrecht JH Smith RG Timchenko NA 《The Journal of biological chemistry》2007,282(2):1468-1478
46.
Akunevich Anastasia Aleksandrovna Khrustalev Vladislav Victorovich Khrustaleva Tatyana Aleksandrovna Poboinev Victor Vitoldovich Shalygo Nikolai Vladimirovich Stojarov Aleksander Nicolaevich Arutyunyan Alexander Migranovich Kordyukova Larisa Valentinovna Sapon Yehor Gennadyevich 《The protein journal》2022,41(2):245-259
The Protein Journal - An interplay between monomeric and dimeric forms of human epidermal growth factor (EGF) affecting its interaction with EGF receptor (EGFR) is poorly understood. While EGF... 相似文献
47.
Aleksei G. Menzorov Konstantin E. Orishchenko Veniamin S. Fishman Anastasia A. Shevtsova Roman V. Mungalov Inna E. Pristyazhnyuk Elena A. Kizilova Natalia M. Matveeva Natalia Alenina Michael Bader Nikolai B. Rubtsov Oleg L. Serov 《Journal of cellular biochemistry》2019,120(10):17208-17218
Neuronal tracing is a modern technology that is based on the expression of fluorescent proteins under the control of cell type–specific promoters. However, random genomic integration of the reporter construct often leads to incorrect spatial and temporal expression of the marker protein. Targeted integration (or knock-in) of the reporter coding sequence is supposed to provide better expression control by exploiting endogenous regulatory elements. Here we describe the generation of two fluorescent reporter systems: enhanced green fluorescent protein (EGFP) under pan-neural marker class III β-tubulin (Tubb3) promoter and mEos2 under serotonergic neuron-specific tryptophan hydroxylase 2 (Tph2) promoter. Differentiation of Tubb3-EGFP embryonic stem (ES) cells into neurons revealed that though Tubb3-positive cells express EGFP, its expression level is not sufficient for the neuronal tracing by routine fluorescent microscopy. Similarly, the expression levels of mEos2-TPH2 in differentiated ES cells was very low and could be detected only on messenger RNA level using polymerase chain reaction-based methods. Our data shows that the use of endogenous regulatory elements to control transgene expression is not always beneficial compared with the random genomic integration. 相似文献
48.
A new species of Tapiridae, Tapirus balkanicus, is etablished for new material of the Bulgarian Upper Miocene. The comparison with other Neogene tapirids leads us to consider the upper jaw as more characteristic than the lower one for taxonomic determination and study of phyletic relations. A new subgenus, Meyeriscus, is created for Tapirus pannonicus, the most evolved European tapirid.
Résumé
Une nouvelle espèce de tapir est décrite, Tapirus balkanicus du Miocène supérieur de Bulgarie. La comparaison avec les espèces européennes du Miocène moyen (Tapirus teilen) au Villafranchien (Tapirus arvernensis) amènent à privilégier la denture supérieure sur l'inférieure pour la détermination et les rapports phylétiques éventuels entre les espèces. Pour l'espèce la plus évoluée de tous les Tapiridés européens, Tapirus pannonicus est proposé un nom de sous-genre particulier, Meyeriscus nov. 相似文献49.
50.