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Viktor Dremin Igor Kozlov Mikhail Volkov Nikita Margaryants Andrey Potemkin Evgeny Zherebtsov Andrey Dunaev Igor Gurov 《Journal of biophotonics》2019,12(6)
The dynamic light scattering methods are widely used in biomedical diagnostics involving evaluation of blood flow. However, there exist some difficulties in quantitative interpretation of backscattered light signals from the viewpoint of diagnostic information. This study considers the application of the high‐speed videocapillaroscopy (VCS) method that provides the direct measurement of the red blood cells (RBCs) velocity into a capillary. The VCS signal presents true oscillation nature of backscattered light caused by moving RBCs. Thus, the VCS signal can be assigned as a reference one with respect to more complicated signals like in laser Doppler flowmetry (LDF). An essential correlation between blood flow velocity oscillations in a separate human capillary and the integral perfusion estimate obtained by the LDF method has been found. The observation of blood flow by the VCS method during upper arm occlusion has shown emergence of the reverse blood flow effect in capillaries that corresponds to the biological zero signal in the LDF. The reverse blood flow effect has to be taken into account in interpretation of LDF signals. 相似文献
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Shtyrlin Valery G. Borissenok Valery A. Serov Nikita Yu. Simakov Vladimir G. Bragunets Vyacheslav A. Trunin Ivan R. Tereshkina Irina A. Koshkin Sergey A. Bukharov Mikhail S. Gilyazetdinov Edward M. Shestakov Evgeny E. Sirotkina Anna G. Zakharov Alexey V. 《Origins of life and evolution of the biosphere》2019,49(1-2):1-18
Origins of Life and Evolution of Biospheres - Syntheses under shock in nitrogen bubbled samples of the water – formamide – bicarbonate – sodium hydroxide system at... 相似文献
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Ivan N. Bolotov Yulia V. Bespalaya Ilya V. Vikhrev Olga V. Aksenova Paul E. Aspholm Mikhail Y. Gofarov Olga K. Klishko Yulia S. Kolosova Alexander V. Kondakov Artyom A. Lyubas Inga S. Paltser Ekaterina S. Konopleva Sakboworn Tumpeesuwan Nikita I. Bolotov Irina S. Voroshilova 《PloS one》2015,10(5)
The freshwater pearl mussel family Margaritiferidae includes 13 extant species, which are all listed by IUCN as endangered or vulnerable taxa. In this study, an extensive spatial sampling of Margaritifera spp. across the Russian Far East (Amur Basin, Kamchatka Peninsula, Kurile Archipelago and Sakhalin Island) was conducted for a revision of their taxonomy and distribution ranges. Based on their DNA sequences, shell and soft tissue morphology, three valid species were identified: Margaritifera dahurica (Middendorff, 1850), M. laevis (Haas, 1910) and M. middendorffi (Rosén, 1926). M. dahurica ranges across the Amur basin and some of the nearest river systems. M. laevis is distributed in Japan, Sakhalin Island and the Kurile Archipelago. M. middendorffi was previously considered an endemic species of the Kamchatka. However, it is widespread in the rivers of Kamchatka, Sakhalin Island, the Kurile Islands (across the Bussol Strait, which is the most significant biogeographical boundary within the archipelago), and, likely, in Japan. The Japanese species M. togakushiensis Kondo & Kobayashi, 2005 seems to be conspecific with M. middendorffi because of similar morphological patterns, small shell size (<100 mm long) and overlapped ranges, but it is in need of a separate revision. Phylogenetic analysis reveals that two NW Pacific margaritiferid species, M. laevis and M. middendorffi, formed a monophyletic 18S rDNA clade together with the North American species M. marrianae and M. falcata. The patterns that were found in these Margaritifera spp. are similar to those of freshwater fishes, indicating multiple colonizations of Eastern Asia by different mitochondrial lineages, including an ancient Beringian exchange between freshwater faunas across the Pacific. 相似文献
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The enzymes involved in the protection of plant metabolism in presence of azo dye was characterized by studying activities of the role of antioxidant enzymes in the hairy roots (HRs) of Physalis minima L. during degradation of an azo dye, Reactive Black 8 (RB8). When the HRs were exposed to RB8 (30 mg L?1), a nine fold increase in SOD activity was observed after 24 h, while 22 and 50 fold increase in activity was observed for POX and APX respectively after 72 h, whereas there was no significant change in activity of CAT. The activation of different antioxidant enzymes at different time intervals under dye stress suggests the synchronized functioning of antioxidant machinery to protect the HRs from oxidative damage. FTIR analysis confirmed the degradation of dye and the non-toxic nature of metabolites formed after dye degradation was confirmed by phytotoxicity study. 相似文献
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Elizabeth Peacock Sarah A. Sonsthagen Martyn E. Obbard Andrei Boltunov Eric V. Regehr Nikita Ovsyanikov Jon Aars Stephen N. Atkinson George K. Sage Andrew G. Hope Eve Zeyl Lutz Bachmann Dorothee Ehrich Kim T. Scribner Steven C. Amstrup Stanislav Belikov Erik W. Born Andrew E. Derocher Ian Stirling Mitchell K. Taylor ?ystein Wiig David Paetkau Sandra L. Talbo 《PloS one》2015,10(8)
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The present paper examines dental diseases and linear enamel hypoplasia among the Garamantes, a Late Holocene Saharan population, and aims to draw conclusions about nutrition and adaptation to a hyper-arid environment. Archaeological evidence suggests that the Garamantian diet included animal protein and local, Mediterranean and Near Eastern plants. Moreoever, although the Garamantes had developed urban centres, the size of these was not large enough to allow for particularly unhygienic conditions to appear. The above archaeological findings were partly corroborated by the current bioarchaeological study. At an intra-population level, the Garamantes showed limited sex differences in dental disease prevalence, while all dental conditions increased in frequency with age, as expected. At an inter-population level, the frequency of all dental conditions was comparable to that found among other North African groups, with the exception of ante-mortem tooth loss. The low frequency of most dental conditions is an indication that the Garamantian diet was overall balanced, while the high frequency of ante-mortem tooth loss may be related to factors such as oral hygiene, food preparation or eating mode, which cannot be controlled for osteologically. Finally, the low frequency of enamel hypoplasia suggests either that the Sahara did not inflict particular stresses on the population, or, more likely, that the Garamantes had developed effective mechanisms for coping with their natural environment. 相似文献
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Nikita A. Kuznetsov Nicolai G. Faleev Alexandra A. Kuznetsova Elena A. Morozova Svetlana V. Revtovich Natalya V. Anufrieva Alexei D. Nikulin Olga S. Fedorova Tatyana V. Demidkina 《The Journal of biological chemistry》2015,290(1):671-681
Methionine γ-lyase (MGL) catalyzes the γ-elimination of l-methionine and its derivatives as well as the β-elimination of l-cysteine and its analogs. These reactions yield α-keto acids and thiols. The mechanism of chemical conversion of amino acids includes numerous reaction intermediates. The detailed analysis of MGL interaction with glycine, l-alanine, l-norvaline, and l-cycloserine was performed by pre-steady-state stopped-flow kinetics. The structure of side chains of the amino acids is important both for their binding with enzyme and for the stability of the external aldimine and ketimine intermediates. X-ray structure of the MGL·l-cycloserine complex has been solved at 1.6 Å resolution. The structure models the ketimine intermediate of physiological reaction. The results elucidate the mechanisms of the intermediate interconversion at the stages of external aldimine and ketimine formation. 相似文献
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