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Field trials were conducted in Rheola Forest, Wales, Great Britain, to determine the effectiveness of Steinernema feltiae UK strain in controlling the web-spinning larch sawfly Cephalcia lariciphila. Foliar sprays at the rate of 5,000-20,000 nematodes/100 cm branch resulted in 3.4-29.4% infection of sawfly larvae. Soil application of 200 nematodes/cm² resulted in 61% infection of sawfly prepupae and 17.3% of pupae. Prepupal infection ranged from 4.8 to 14.7% 1 year after nematode application. Soil applications of this nematode show that it has potential for biological control of sawfly prepupae. 相似文献
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The AKin10 gene from Arabidopsis thaliana encoding a putative Ser/Thr protein kinase (PK) has been isolated and characterized. The AKin10-encoding gene is located on a genomic 5.4-kb BamHI fragment and contains ten introns, one being located in the 5' untranslated region. The deduced amino acid sequence of AKin10 is 65% identical over the catalytic domain to the yeast PK (SNF1). SNF1 is essential for the derepression of many glucose-repressible genes, including Suc2 which encodes invertase. Southern blot hybridization experiments suggested the presence of one copy of the gene per haploid genome of A. thaliana. Northern hybridization experiments indicated that this gene is expressed in roots, shoots and leaves. AKin10 may play an important role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. 相似文献
25.
Synopsis Changes in the daily appetite and weekly growth rates of individual adult minnows,Phoxinus phoxinus, on ad libitum rations were recorded before and after they had experienced 4 or 16 days of food restriction. Feeding levels during the restriction periods were either starvation or a maintenance ration. The latter was estimated from a previously determined regression model. Water temperature was 15°C and the photoperiod 9L15D in all experiments. The mean weight of fish used ranged from 1.06 to 2.15 g. The 4 day restriction had no detectable effects on appetite or growth. After the 16 day restriction, the minnows showed hyperphagia and had increased specific growth rates and growth efficiencies compared with control fish. The compensatory increases in appetite and growth were not sustained and within three weeks had declined to levels not significantly different from those of the control fish. At the end of the experiments, there were no significant differences between the mean weights or cumulative food consumption of the restricted and control groups. The results suggest that adult minnows regulate their appetite and growth rate in relation to their previous nutritional history. 相似文献
26.
Nigel A. S. Taylor James D. Cotter Stephen N. Stanley Robert N. Marshall 《European journal of applied physiology and occupational physiology》1991,62(2):116-121
Technical limitations of some isokinetic dynamometers have called into question the validity of some data on human muscle mechanics. The Biodex dynamometer has been shown to minimize the impact artefact while permitting automatic gravity correction. This dynamometer was used to study quadriceps muscle torque and power generation in elite power (n = 6) and elite endurance (n = 7) athletes over 12 randomly assigned isokinetic velocities from 30 degrees.s-1 to 300 degrees.s-1. The angle at peak torque varied as a negative, linear function of angular velocity, with the average angle across test velocities being 59.5 degrees (SD 10.2 degrees). Power athletes developed greater peak torque at each angular velocity (P less than 0.05) and experienced a 39.7% decrement in torque over the velocity range tested. Endurance athletes encountered a 38.8% decline in peak torque. Torques measured at 60 degrees of knee flexion followed a similar trend in both groups; however the greatest torques were recorded at 60 degrees.s-1 rather than at 30 degrees.s-1. Leg extensor muscle power increased monotonically with angular velocity in both power (r2 = 0.728) and endurance athletes (r2 = 0.839); however these curves diverged significantly so that the power athletes produced progressively more power with each velocity increment. These inter group differences probably reflected a combination of natural selection and training adaptation. 相似文献
27.
Addition of 2-(3-chloro-4-trifluoromethyl)anilino-3,5-dinitrothiophene (ANT2p) to detergent-solubilised Photosystem II (PS II) particles results in the photo-oxidation of carotenoid and inhibition of the steady-state oxygen-evolution rate. It has been proposed that ANT2p may modify the water-splitting reactions by mediating the transfer of reducing equivalents from endogenous electron donors, such as carotenoid, to the S2 and S3 oxidation states of PS II. In this paper we present evidence indicating that ANT2p can interact with PS II at two separate loci. The water-splitting complex is shown to be the primary site of attack by ANT2p, since artificial electron donors, such as 1,5-diphenylcarbazide (DPC), can restore PS II photochemical activity by feeding reducing equivalents directly to the reaction centre. The ANT2p interaction at this site is light-intensity dependent. A second inhibitory site close to the reaction centre P-680 chlorophyll is detected at slightly higher ANT2p concentrations. The inhibition at this site is unaffected either by changes in the actinic light intensity or by the addition of electron donors. The flash-induced oxidation of carotenoid has an ANT2p concentration dependence and an insensitivity to DPC which suggests that it results from the inhibition of the reaction centre and not with that of the water-splitting complex. 相似文献
28.
Nigel L. Brown Tapan K. Misra Joseph N. Winnie Annette Schmidt Michael Seiff Simon Silver 《Molecular & general genetics : MGG》1986,202(1):143-151
Summary The DNA sequences of the mercuric resistance determinants of plasmid R100 and transposon Tn501 distal to the gene (merA) coding for mercuric reductase have been determined. These 1.4 kilobase (kb) regions show 79% identity in their nucleotide sequence and in both sequences two common potential coding sequences have been identified. In R100, the end of the homologous sequence is disrupted by an 11.2 kb segment of DNA which encodes the sulfonamide and streptomycin resistance determinants of Tn21. This insert contains terminal inverted repeat sequences and is flanked by a 5 base pair (bp) direct repeat. The first of the common potential coding sequences is likely to be that of the merD gene. Induction experiments and mercury volatilization studies demonstrate an enhancing but non-essential role for these merA-distal coding sequences in mercury resistance and volatilization. The potential coding sequences have predicted codon usages similar to those found in other Tn501 and R100 mer genes. 相似文献
29.
Astrid G. Chapman Michael J. Halsey Geneen P. Hart Nigel P. Luff Brian S. Meldrum Bridget Wardley-Smith 《Journal of neurochemistry》1986,47(1):314-317
Regional amino acid concentrations were measured in rat brain fixed by microwave irradiation at three levels of elevated atmospheric pressure corresponding to different phases of the high-pressure neurological syndrome [20 atmospheres absolute (ATA), no clinical signs; 60 ATA, tremor; 85 ATA, severe tremor and myoclonic jerks]. No changes in amino acid content occurred at 20 or 60 ATA. At 85 ATA glutamine content increased in hippocampus, striatum, cerebellum, and substantia nigra, and gamma-aminobutyric acid content increased in hippocampus. It is suggested that enhanced glutamate release in various subcortical structures contributes to the myoclonic activity observed at 85 ATA. 相似文献
30.
The major sesquiterpenes in the foliage of Dacrydium cupressinum are α-longipinene, longifolene, longibornyl acetate, caryophyllene, caryophyllene oxide, humulene, α- and β-selinene, β- and δ-elemene, aromadendrene and the rare 9βH-caryophyllene. Sesquiterpene levels vary greatly from tree to tree. As this variation is largely independent of environmental factors, genetic control is proposed. Longifolene and α-longipinene levels are closely correlated, as are those of caryophyllene and humulene. The biosynthetic implications of these correlations are discussed. 相似文献