全文获取类型
收费全文 | 674篇 |
免费 | 43篇 |
专业分类
717篇 |
出版年
2024年 | 2篇 |
2023年 | 8篇 |
2022年 | 12篇 |
2021年 | 25篇 |
2020年 | 15篇 |
2019年 | 18篇 |
2018年 | 26篇 |
2017年 | 25篇 |
2016年 | 25篇 |
2015年 | 50篇 |
2014年 | 43篇 |
2013年 | 52篇 |
2012年 | 81篇 |
2011年 | 67篇 |
2010年 | 36篇 |
2009年 | 23篇 |
2008年 | 30篇 |
2007年 | 35篇 |
2006年 | 23篇 |
2005年 | 26篇 |
2004年 | 24篇 |
2003年 | 11篇 |
2002年 | 15篇 |
2001年 | 10篇 |
2000年 | 5篇 |
1999年 | 2篇 |
1998年 | 3篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1993年 | 4篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1988年 | 3篇 |
1987年 | 1篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1979年 | 2篇 |
1977年 | 1篇 |
排序方式: 共有717条查询结果,搜索用时 0 毫秒
711.
Kaihnsa Nidhi Ren Yue Safey El Din Mohab Martini Johannes W. R. 《Journal of mathematical biology》2020,81(4-5):1169-1191
We consider a measure of cooperativity based on the minimal interaction required to generate an observed titration behavior. We describe the corresponding algebraic optimization problem and show how it can be solved using the nonlinear algebra tool SCIP. Moreover, we compute the minimal interactions and minimal molecules for several binding polynomials that describe the oxygen binding of various hemoglobins under different conditions. We compare their minimal interaction with the maximal slope of the Hill plot, and discuss similarities and discrepancies with a view towards the shapes of the binding curves.
相似文献712.
Large genomic sequencing projects of pathogens as well as human genome leads to immense genomic and proteomic data which would be very
beneficial for the novel target identification in pathogens. Subtractive genomic approach is one of the most useful strategies helpful in
identification of potential targets. The approach works by subtracting the genes or proteins homologous to both host and the pathogen and
identify those set of gene or proteins which are essential for the pathogen and are exclusively present in the pathogen. Subtractive genomic
approach is employed to identify novel target in salmonella typhi. The pathogen has 4718 proteins out of which 300 are found to be essential
(“ indispensable to support cellular life”) in the pathogen with no human homolog. Metabolic pathway analyses of these 300 essential proteins
revealed that 149 proteins are exclusively involved in several metabolic pathway of S. typhi. 8 metabolic pathways are found to be present
exclusively in the pathogen comprising of 27 enzymes unique to the pathogen. Thus, these 27 proteins may serve as prospective drug targets.
Sub-cellular localization prediction of the 300 essential proteins was done which reveals that 11 proteins lie on the outer membrane of the
pathogen which could be probable vaccine candidates. 相似文献
713.
A new strain of Bacillus sp. I-3, isolated from natural soil samples, showed a high raw starch digesting activity towards potato starch. Upon optimization of various environmental and cultural conditions, the yield of α-amylase reached 642 U/mL. The kinetic characterization of partially purified enzyme exhibited the maximum activity at 70 °C, pH 7.0 and revealed a high thermostability in the presence of 10 mM CaCl2·2H2O where it could retain more than 90% residual activity at 70 °C after 3.5 h. At 80, 90 and 100 °C, the enzyme retained 80, 59 and 26% of its maximum activity after 2.5, 0.5 and 0.5 h, respectively. The enzyme preparation had a strong affinity towards raw potato starch granules and was almost completely adsorbed onto it. It also hydrolyzed raw potato starch at a concentration of 12.5% significantly in a short period of time of 12 h. 相似文献
714.
715.
716.
717.
S Purohit P Kumar M Laloraya S Bharti M M Laloraya 《Biochemical and biophysical research communications》1992,186(2):652-658
The dynamics of stomatal opening and closure had to date been ascribed largely to the K(+)-fluxes and cell wall elasticity. Using protoplasts of guard cells of Vicia faba as model system, we document convincing first hand evidence a that lipid phase alterations could regulate ABA-induced closure of stomates and its reversal by umbelliferone. Backed up by the presence of plasmalemma-located ABA-receptor in guard cells, a novel theory could be put forth explaining guard cell opening and closure mediated by hormone induced reconfiguration via a probable lipid-protein lattice modification. The phase reversal of the plasmalemma by umbelliferone is postulated to be through modified hormone receptor complex structure, which is yet to be substantiated. 相似文献