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51.
Anjana Sharma Sachin Kumar Chandraker Manisha Bharti 《Indian journal of microbiology》2007,47(3):233-240
Sixteen isolates of Nontubercular Mycobacteria species were isolated from drinking water supply of some educational institutes
in Jabalpur during July 2006 and were identified by biochemical test, thin layer chromatography (TLC) and high performance
liquid chromatography (HPLC) analysis and PRA ( PCR restriction enzyme analysis) of rpoB gene. Out of 21 water samples total
16 isolates of nontuberculous mycobacteria were identified, as M. terrae (6), M. szulgai (4), M. gordonae (3), and one each as M. malmoense, M. kansasii, and M. gastri. 相似文献
52.
53.
Sultan M Piccini I Balzereit D Herwig R Saran NG Lehrach H Reeves RH Yaspo ML 《Genome biology》2007,8(5):R91
Background
Down's syndrome (DS), or trisomy 21, is a complex developmental disorder that exhibits many clinical signs that vary in occurrence and severity among patients. The molecular mechanisms responsible for DS have thus far remained elusive. We argue here that normal variation in gene expression in the population contributes to the heterogeneous clinical picture of DS, and we estimated the amplitude of this variation in 50 mouse orthologs of chromosome 21 genes in brain regions of Ts65Dn (a mouse model of DS). We analyzed the RNAs of eight Ts65Dn and eight euploid mice by real-time polymerase chain reaction. 相似文献54.
A multiple-unit-type oral floating dosage form (FDF) of 5-fluorouracil (5-FU) was developed to prolong gastric residence time,
target stomach cancer, and increase drug bioavailability. The floating bead formulations were prepared by dispersing 5-FU
together with calcium carbonate into a mixture of sodium alginate and hydroxypropyl methylcellulose solution and then dripping
the dispersion into an acidified solution of calcium chloride. Calcium alginate beads were formed, as alginate undergoes ionotropic
gelation by calcium ions and carbon dioxide develops from the reaction of carbonate salts with acid. The evolving gas permeated
through the alginate matrix, leaving gas bubbles or pores, which provided the beads buoyancy. The prepared beads were evaluated
for percent drug loading, drug entrapment efficiency, image, surface topography, buoyancy, and in vitro release. The formulations
were optimized for different weight ratios of gas-forming agent and sodium alginate. The beads containing higher amounts of
calcium carbonate demonstrated instantaneous, complete, and excellent floating ability over a period of 24 hours. The optimized
formulation was subjected to in vivo antitumor studies to check the therapeutic efficacy of the floating dosage forms containing
5-FU against benzo(a)pyrene-induced stomach tumors in albino female mice (Balb/C strain). The multiple-bead FDF was found
to reduce the tumor incidence in mice by 74%, while the conventional tablet dosage form reduced this incidence by only 25%.
Results indicate that FDF performed significantly better than the simple tablet dosage form.
Published: June 22, 2007 相似文献
55.
Jason J. Rudd Kostya Kanyuka Keywan Hassani-Pak Mark Derbyshire Ambrose Andongabo Jean Devonshire Artem Lysenko Mansoor Saqi Nalini M. Desai Stephen J. Powers Juliet Hooper Linda Ambroso Arvind Bharti Andrew Farmer Kim E. Hammond-Kosack Robert A. Dietrich Mikael Courbot 《Plant physiology》2015,167(3):1158-1185
56.
57.
58.
Amresh Kumar Nidhi N. Prasad Subodh Kumar Sinha 《Physiology and Molecular Biology of Plants》2015,21(1):159-162
Eleven germplasms of faba bean seeds from four agroclimatic regions of Bihar, India, have been investigated to estimate their nutritional (soluble protein, free amino acids, starch, reducing and non reducing sugar, total soluble sugar) and antinutritional (total extractable phenol and condensed tannin/proanthocyanidin) parameters. These parameters were found in varying concentration in all genotypes studied. The highest concentration of total extractable phenol and proanthocyanidin (condensed tannin) (2.56 and 1.59 % leucocyanidin equivalents respectively on dry matter basis) were found in Samastipur while the lowest from Patna (0.95 and 0.426 % leucocyanidin equivalent on dry matter basis). The different nutritional parameters were also found to be in variable concentration among different germplasms viz. total soluble protein ≈ 20–32 %, free amino acids ≈ 188–348 mg/100 g, starch ≈ 27–33 %, reducing sugars ≈ 85–188 mg/100 g, non reducing sugars ≈ 0.7–1.7 % and total soluble sugars ≈ 0.8–1.9 %. 相似文献
59.
Four species of the ant genus Ponera Latreille, 1804, are recorded from India. The present study reports one new species Ponera
sikkimensis
sp. n., a divergent population of Ponera
indica Bharti & Wachkoo, 2012 and one new record, Ponera
paedericera Zhou, 2001 from India. An identification key and distributions for the four known Indian species of Ponera based on the worker caste are provided. 相似文献
60.
Qianzheng Zhu Nidhi Sharma Jinshan He Gulzar Wani Altaf A Wani 《Cell cycle (Georgetown, Tex.)》2015,14(9):1413-1425
During DNA damage response (DDR), histone ubiquitination by RNF168 is a critical event, which orchestrates the recruitment of downstream DDR factors, e.g. BRCA1 and 53BP1. Here, we report USP7 deubiquitinase regulates the stability of RNF168. We showed that USP7 disruption impairs H2A and ultraviolet radiation (UVR)-induced γH2AX monoubiquitination, and decreases the levels of pBmi1, Bmi1, RNF168 and BRCA1. The effect of USP7 disruption was recapitulated by siRNA-mediated USP7 depletion. The USP7 disruption also compromises the formation of UVR-induced foci (UVRIF) and ionizing radiation-induced foci (IRIF) of monoubiquitinated H2A (uH2A) and polyubiquitinated H2AX/A, and subsequently affects UVRIF and IRIF of BRCA1 as well as the IRIF of 53BP1. USP7 was shown to physically bind RNF168 in vitro and in vivo. Overexpression of wild-type USP7, but not its interaction-defective mutant, prevents UVR-induced RNF168 degradation. The USP7 mutant is unable to cleave Ub-conjugates of RNF168 in vivo. Importantly, ectopic expression of RNF168, or both RNF8 and RNF168 together in USP7-disrupted cells, significantly rescue the formation of UVRIF and IRIF of polyubiquitinated H2A and BRCA1. Taken together, these findings reveal an important role of USP7 in regulating ubiquitin-dependent signaling via stabilization of RNF168. 相似文献